Preparation and characterization of unilamellar myelin vesicles.

Myelin vesicles have been obtained from isolated rat brain myelin and shown by electron microscopy to consist of single bilayer membranes. The yield of the preparation is approximately 25% of the myelin proteins. The vesicles show a typical myelin protein pattern on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contain activity for the myelin marker enzyme, 2',3'-cyclic nucleotide-3'-phosphohydrolase (CNPase). The preparation consists of both inside-out and right-side-out vesicles, and the proportion in each orientation varies from one preparation to another. The occurrence of two populations is demonstrated by the observation that hypotonically lysed vesicles compete to a greater extent than intact vesicles in a competitive enzyme-linked immunosorbent assay with myelin basic protein antiserum. In addition, only a portion of the CNPase activity of the vesicles is trypsin-sensitive and detectable in the absence of detergent; the remaining, trypsin-insensitive activity is present in detergent-disrupted membranes. Thus, there are vesicle populations in which myelin basic protein and CNPase are accessible and others in which they are inaccessible. A population of uniformly oriented right-side-out vesicles has been obtained by ConA-Agarose affinity column chromatography and elution of the bound fraction with methyl-alpha-D-mannopyranoside. In the absence of detergent, less than 10% of the total CNPase activity of these vesicles can be demonstrated, suggesting that the active site of CNPase is opposite to that of the ConA binding site and, therefore, appears to be on the cytoplasmic face of the myelin membrane.

• Publication year

  1986

• Venue

  Journal of Biological Chemistry

• Publication date

  1986-12-05

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)66705-5PMID 2430966
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Stabilization of lipid bilayer vesicles by sucrose during freezing.

  1986cited by this paper
• An immunologic probe for a defined region of the myelin proteolipid.

  1985cited by this paper
• Resistance to Disruption of Multilamellar Fragments of Central Nervous System Myelin

  1984cited by this paper
• Interactions of dicyclohexylcarbodiimide with myelin proteolipid.

  1982cited by this paper
• Liposomes with a large trapping capacity prepared by freezing and thawing of sonicated phospholipid mixtures.

  1981cited by this paper
• Myelin-associated glycoprotein demonstrated immunocytochemically in myelin and myelin-forming cells of developing rat.

  1979cited by this paper
• Preparation of membrane vesicles from isolated myelin: studies on functional and structural properties.

  1978cited by this paper
• 2′,3′‐CYCLIC NADP AS A SUBSTRATE FOR 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE

  1976cited by this paper
• Topographical arrangement of membrane proteins in the intact myelin sheath. Lactoperoxidase incorproation of iodine into myelin surface proteins.

  1975cited by this paper
• MYELINATION IN RAT BRAIN: METHOD OF MYELIN ISOLATION 1

  1973cited by this paper
• Modification of the Lowry procedure for the analysis of proteolipid protein.

  1972cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Nano-structured myelin: new nanovesicles for targeted delivery to white matter and microglia, from brain-to-brain

  2021cites this paper
• Mast cell activation by myelin through scavenger receptor.

  2008cites this paper
• Conformation of brain proteolipid apoprotein

  2004cites this paper
• Ionic fluxes through myelin membrane vesicles

  1996cites this paper
• Preparation of Giant Myelin Vesicles and Proteoliposomes to Register Ionic Channels

  1996cites this paper
• Myelin vesicles: What we know and what we do not know

  1995cites this paper
• 2′, 3′-Cyclic-nucleotide 3′-phosphodiesterase

  1991cites this paper
• 2',3'-cyclic nucleotide 3'-phosphodiesterase, an oligodendrocyte-Schwann cell and myelin-associated enzyme of the nervous system.

  1989cites this paper
• Immunochemical characterization of antibodies to the myelin proteolipid protein (PLP).

  1988cites this paper
• Immunochemical differentiation of highly conserved forms of myelin proteolipid.

  1988cites this paper
• Chapter 11 – MYELIN PROTEOLIPID PROTEIN

  1988cites this paper