Genes Expressed in Ascochyta raftiei-Inoculated Chickpea Plants and Elicited Cell Cultures as Detected by Differential cDNA-Hybridization

Y. Ichinose,K. Tiemann,C. Schwenger-Erger,K. Toyoda,Frauke Hein,T. Hanselle,Holger Cornels,W. Barz

Published 2000 in Zeitschrift für Naturforschung C - A Journal of Biosciences

ABSTRACT

Abstract In response to the exogenous application of elicitors and attempted invasion by pathogens, plants exhibit a wide range of defense reactions. To understand the defense mechanism s at the level of gene activation and deactivation, differential screenings were perform ed to isolate cDNA clones which are differentially expressed in pathogen-inoculated resistant chickpea plants and elicitor-treated cell cultures. A plenty of genes were isolated and arranged in 5 groups, namely defense-related pathways, signal transduction pathways, regulation of gene expression, catabolic pathways and primary metabolism . Most of these genes were activated although several genes were also found to be suppressed. We discuss the plausible functions of cDNA products in plant defense responses. The cDNA s provide a variety of tools to investigate m olecular mechanisms of defense responses and clearly reflect the massive genomic and metabolic changes which occur during manifestation of antimicrobial defense.

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