Long-range electrostatic interactions can influence the folding, stability, and cooperativity of dihydrofolate reductase.

No abstract is available for this paper.

• Publication year

  1989

• Venue

  Biochemistry

• Publication date

  1989-09-19

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI00445A061PMID 2692706
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Ribonuclease T1 is stabilized by cation and anion binding.

  1988cited by this paper
• Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium.

  1988cited by this paper
• Stability mutants of staphylococcal nuclease: large compensating enthalpy-entropy changes for the reversible denaturation reaction.

  1988cited by this paper
• Ribonuclease T1 is stabilized by cation and anion binding.

  1988cited by this paper
• Effect of point mutations on the folding of globular proteins.

  1987cited by this paper
• Effect of point mutations on the folding of globular proteins.

  1987cited by this paper
• Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering.

  1987cited by this paper
• Effect of single amino acid replacements on the folding and stability of dihydrofolate reductase from Escherichia coli.

  1987cited by this paper
• Synergism in folding of a double mutant of the alpha subunit of tryptophan synthase.

  1986cited by this paper
• Stabilization of λ repressor against thermal denaturation by site‐directed Gly→Ala changes in α‐helix 3

  1986cited by this paper
• Effects of the phenylalanine-22----leucine, glutamic acid-49----methionine, glycine-234----aspartic acid, and glycine-234----lysine mutations on the folding and stability of the alpha subunit of tryptophan synthase from Escherichia coli.

  1986cited by this paper
• Synergism in folding of a double mutant of the alpha subunit of tryptophan synthase.

  1986cited by this paper
• Folding of dihydrofolate reductase from Escherichia coli

  1986cited by this paper
• Folding of dihydrofolate reductase from Escherichia coli.

  1986cited by this paper
• Stabilization of lambda repressor against thermal denaturation by site-directed Gly----Ala changes in alpha-helix 3.

  1986cited by this paper
• Functional role of aspartic acid-27 in dihydrofolate reductase revealed by mutagenesis.

  1986cited by this paper
• Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation

  1986cited by this paper
• Effects of site-specific amino acid modification on protein interactions and biological function.

  1985cited by this paper
• EFFECTS OF SITE-SPECIFIC AMINO ACID MODIFICATION ON PROTEIN INTERACTIONS AND BIOLOGICAL FUNCTION

  1985cited by this paper
• Electrostatic interactions in globular proteins: calculation of the pH dependence of the redox potential of cytochrome c551.

  1985cited by this paper
• Catalytic mechanism of the dihydrofolate reductase reaction as determined by pH studies.

  1984cited by this paper
• Conformational stability of mixed disulfide derivatives of .beta.-lactoglobulin B

  1983cited by this paper
• Conformational stability of mixed disulfide derivatives of beta-lactoglobulin B.

  1983cited by this paper
• Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate.

  1982cited by this paper
• Specific intermediates in the folding reactions of small proteins and the mechanism of protein folding.

  1982cited by this paper
• Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.

  1980cited by this paper
• Escherichia coli dihydrofolate reductase: isolation and characterization of two isozymes.

  1977cited by this paper
• The mechanism of folding of globular proteins. Equilibria and kinetics of conformational transitions of penicillinase from Staphylococcus aureus involving a state of intermediate conformation.

  1976cited by this paper
• Purification and properties of Escherichia coli dihydrofolate reductase.

  1975cited by this paper
• Denaturation of bovine carbonic anhydrase B by guanidine hydrochloride. A process involving separable sequential conformational transitions.

  1973cited by this paper
• Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase.

  1967cited by this paper
• Some factors in the interpretation of protein denaturation.

  1959cited by this paper

• DNA framework-engineered long-range electrostatic interactions for DNA hybridization reactions.

  2021cites this paper
• One Plus One Makes Three: Triangular Coupling of Correlated Amino Acid Mutations.

  2021cites this paper
• The expression and functional analysis of the sialyl-T antigen in prostate cancer

  2020cites this paper
• Model investigations for vanadium-protein interactions: Synthesis, characterization and antidiabetic properties

  2019cites this paper
• How a multimeric macromolecule is affected by divalent salts? Experimental and simulation study.

  2018cites this paper
• ASSAYS IN WHOLE ANIMALS

  2017cites this paper
• COMPLEMENTATIONASSAYS FOR THE DETECTION OF BIOLOGICAL OR DRUG INTERACTIONS

  2017cites this paper
• Thioredoxin from Escherichia coli as a Role Model of Molecular Recognition, Folding, Dynamics and Function.

  2015cites this paper
• Quality Matters: Extension of Clusters of Residues with Good Hydrophobic Contacts Stabilize (Hyper)Thermophilic Proteins

  2014cites this paper
• From systems to structure: bridging networks and mechanism.

  2013cites this paper
• Identification and characterization of molten globule-like state of hen egg-white lysozyme in presence of salts under alkaline conditions.

  2010cites this paper
• Urea denatured state ensembles contain extensive secondary structure that is increased in hydrophobic proteins

  2010cites this paper
• Interaction of the Aromatics Tyr-72/Trp-288 in the Interface of the Extracellular and Transmembrane Domains Is Essential for Proton Gating of Acid-sensing Ion Channels

  2009cites this paper
• Sequence perturbation analysis: addressing amino acid indices to elucidate the C-terminal role of Escherichia coli dihydrofolate reductase.

  2009cites this paper
• Use of protein engineering techniques to elucidate protein folding pathways.

  2008cites this paper
• MEMBRANE PROTEIN FOLDING: THE ROLE OF AMINO ACID SEQUENCE IN SPECIFYING THE STABILITY AND CONFORMATION OF TRANSMEMBRANE OLIGOMERS

  2006cites this paper
• Evolutional Design of a Hyperactive Cysteine- and Methionine-free Mutant of Escherichia coli Dihydrofolate Reductase*

  2006cites this paper
• Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding

  2005cites this paper
• Complex interactions at the helix-helix interface stabilize the glycophorin A transmembrane dimer.

  2004cites this paper
• The problem of amino acid complementarity and antisense peptides.

  2004cites this paper
• Übersetzung der europäischen Patentschrift

  2004cites this paper
• Conferring thermostability to mesophilic proteins through optimized electrostatic surfaces.

  2003cites this paper
• Optimized electrostatic surfaces parallel increased thermostability: a structural bioinformatic analysis.

  2003cites this paper
• Free solution electrophoresis measurements and their theoretical relationships to net protein valence

  2003cites this paper
• Thermodynamic analysis of the contributions of the copper ion and the disulfide bridge to azurin stability: synergism among multiple depletions.

  2003cites this paper
• The effect of salts on the activity and stability of Escherichia coli and Haloferax volcanii dihydrofolate reductases.

  2002cites this paper
• Divalent cation induced changes in structural properties of the dimeric enzyme glucose oxidase: dual effect of dimer stabilization and dissociation with loss of cooperative interactions in enzyme monomer.

  2002cites this paper
• Monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme.

  2001cites this paper
• Asymmetric and Independent Contribution of the Second Transmembrane Segment 12′ Residues to Diliganded Gating of Acetylcholine Receptor Channels

  2000cites this paper
• Effect of ligand binding on the flexibility of dihydrofolate reductase as revealed by compressibility.

  2000cites this paper
• Engineering a thermostable protein via optimization of charge-charge interactions on the protein surface.

  1999cites this paper
• High-throughput sample preparation for protein or peptide structural characterization.

  1999cites this paper
• High-throughput sample preparation for protein or peptide structural characterization.

  1999cites this paper
• Evolutionarily conserved pathways of energetic connectivity in protein families.

  1999cites this paper
• Effects of the T→R transition on the electrostatic properties of E. coli aspartate transcarbamylase

  1998cites this paper
• Site-specific analysis of mutational effects in proteins.

  1998cites this paper
• Refolding of [6-19F]tryptophan-labeled Escherichia coli dihydrofolate reductase in the presence of ligand: a stopped-flow NMR spectroscopy study.

  1998cites this paper
• Site-specific analysis of mutational effects in proteins.

  1998cites this paper
• Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of Escherichia coli dihydrofolate reductase on its stability and function.

  1998cites this paper
• Effects of point mutations at the flexible loop alanine-145 of Escherichia coli dihydrofolate reductase on its stability and function.

  1998cites this paper
• Mutational analysis of the BPTI folding pathway: II. Effects of aromatic → leucine substitutions on folding kinetics and thermodynamics

  1997cites this paper
• Thermodynamics of denaturation of staphylococcal nuclease mutants: an intermediate state in protein folding

  1996cites this paper
• Homology modelling of rat kallikrein rK9, a member of the tissue kallikrein family: implications for substrate specificity and inhibitor binding.

  1996cites this paper
• Circularly permuted dihydrofolate reductase possesses all the properties of the molten globule state, but can resume functional tertiary structure by interaction with its ligands

  1996cites this paper
• Acid and thermal unfolding of Escherichia coli dihydrofolate reductase.

  1996cites this paper
• Long-range, small magnitude nonadditivity of mutational effects in proteins.

  1995cites this paper
• Energetics of denaturation and m values of staphylococcal nuclease mutants.

  1995cites this paper
• Staphylococcal nuclease: a showcase of m-value effects.

  1995cites this paper
• Probing the determinants of protein stability: comparison of class A beta-lactamases.

  1995cites this paper
• Molten globule and protein folding.

  1995cites this paper
• Investigating the role of conserved residue Asp134 in Escherichia coli ribonuclease HI by site-directed random mutagenesis.

  1994cites this paper
• Development of nonpolar surfaces in the folding of Escherichia coli dihydrofolate reductase detected by 1-anilinonaphthalene-8-sulfonate binding.

  1994cites this paper
• Receptor binding properties of four‐helix‐bundle growth factors deduced from electrostatic analysis

  1994cites this paper
• Comparison of kinetics of formation of helices and hydrophobic core during the folding of staphylococcal nuclease from acid.

  1994cites this paper
• Point mutations at glycine-121 of Escherichia coli dihydrofolate reductase: important roles of a flexible loop in the stability and function.

  1994cites this paper
• 19F NMR spectroscopy of [6-19F]tryptophan-labeled Escherichia coli dihydrofolate reductase: equilibrium folding and ligand binding studies.

  1994cites this paper
• pH-dependent thermostabilization of Escherichia coli ribonuclease HI by histidine to alanine substitutions.

  1993cites this paper
• Pathways of protein folding.

  1993cites this paper
• Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'.

  1993cites this paper
• Patterns of nonadditivity between pairs of stability mutations in staphylococcal nuclease.

  1993cites this paper
• Protein engineering strategies in examining protein folding intermediates: Current opinion in Structural Biology 1993, 3:594–600

  1993cites this paper
• External ions and direct membrane effects of enkephalins on Amoeba proteus.

  1993cites this paper
• Molecular simulations of peptide and protein unfolding: in quest of a molten globule

  1993cites this paper
• Effects of point mutation in a flexible loop on the stability and enzymatic function of Escherichia coli dihydrofolate reductase.

  1993cites this paper
• Folding kinetics of T4 lysozyme and nine mutants at 12 degrees C.

  1992cites this paper
• Contributions of the polar, uncharged amino acids to the stability of staphylococcal nuclease: evidence for mutational effects on the free energy of the denatured state.

  1992cites this paper
• Partial denaturation of transthyretin is sufficient for amyloid fibril formation in vitro.

  1992cites this paper
• Mutational studies of protein structures and their stabilities

  1992cites this paper
• Beta-sheet rearrangements: serpins and beyond.

  1992cites this paper
• Stability of folded conformations

  1991cites this paper
• Effects of point mutations in a hinge region on the stability, folding, and enzymatic activity of Escherichia coli dihydrofolate reductase.

  1991cites this paper
• Modification of enzyme catalysis by engineering surface charge.

  1991cites this paper
• Role of electrostatic interaction in the stability of the hexamer of constitutive phycocyanin from Fremyella diplosiphon.

  1991cites this paper
• Isolation and in vitro characterization of temperature-sensitive mutants of the bacteriophage f1 gene V protein.

  1991cites this paper
• Electrostatic effects in protein folding, stability, and function.

  1991cites this paper
• Long-range interactions in proteins.

  1991cites this paper
• Transient intermediates in the folding of dihydrofolate reductase as detected by far-ultraviolet circular dichroism spectroscopy.

  1991cites this paper
• Cumulative site-directed charge-change replacements in bacteriophage T4 lysozyme suggest that long-range electrostatic interactions contribute little to protein stability.

  1991cites this paper
• Contribution of histidine residues to the conformational stability of ribonuclease T1 and mutant Glu-58----Ala.

  1990cites this paper
• Strategy for analysing the co-operativity of intramolecular interactions in peptides and proteins.

  1990cites this paper
• Protein surface charges and Ca2+ binding to individual sites in calbindin D9k: stopped-flow studies.

  1990cites this paper
• Refolding of Escherichia coli dihydrofolate reductase: sequential formation of substrate binding sites.

  1990cites this paper
• Kinetic and conformational effects of lysine substitutions for arginines 35 and 87 in the active site of staphylococcal nuclease.

  1990cites this paper