A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus

BackgroundElephants are classified as critically endangered animals by the International Union for Conservation of Species (IUCN). Elephant endotheliotropic herpesvirus (EEHV) poses a large threat to breeding programs of captive Asian elephants by causing fatal haemorrhagic disease. EEHV infection is detected by PCR in samples from both clinically ill and asymptomatic elephants with an active infection, whereas latent carriers can be distinguished exclusively via serological assays. To date, identification of latent carriers has been challenging, since there are no serological assays capable of detecting seropositive elephants.ResultsHere we describe a novel ELISA that specifically detects EEHV antibodies circulating in Asian elephant plasma/serum. Approximately 80 % of PCR positive elephants display EEHV-specific antibodies. Monitoring three Asian elephant herds from European zoos revealed that the serostatus of elephants within a herd varied from non-detectable to high titers. The antibody titers showed typical herpes-like rise-and-fall patterns in time which occur in all seropositive animals in the herd more or less simultaneously.ConclusionsThis study shows that the developed ELISA is suitable to detect antibodies specific to EEHV. It allows study of EEHV seroprevalence in Asian elephants. Results confirm that EEHV prevalence among Asian elephants (whether captive-born or wild-caught) is high.

• Publication year

  2015

• Venue

  BMC Veterinary Research

• Publication date

  2015-08-14

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s12917-015-0522-6PMID 26268467PMCID 4535388
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Nervous system viral infections in immunocompromised hosts.

  2014cited by this paper
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  2014cited by this paper
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  2013cited by this paper
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  2013cited by this paper
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  2013cited by this paper
• A potentially fatal mix of herpes in zoos.

  2012cited by this paper
• Detection and evaluation of novel herpesviruses in routine and pathological samples from Asian and African elephants: identification of two new probosciviruses (EEHV5 and EEHV6) and two new gammaherpesviruses (EGHV3B and EGHV5).

  2011cited by this paper
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  2011cited by this paper
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  2010cited by this paper
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  2010cited by this paper
• The order Herpesvirales

  2008cited by this paper
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  2008cited by this paper
• Comparison of glycoprotein B (gB) variants of the elephant endotheliotropic herpesvirus (EEHV) isolated from Asian elephants (Elephas maximus).

  2003influential reference
• Evidence that the bypassing ribosome travels through the coding gap

  2003cited by this paper
• Novel endotheliotropic herpesviruses fatal for Asian and African elephants.

  1999cited by this paper
• Functional importance of RNA interactions in selection of translation initiation codons

  1997cited by this paper
• Serological Evidence of Bovine Herpesviruses 1 and 2 in Asian Elephants

  1997cited by this paper
• Masters of deception: A review of herpesvirus immune evasion strategies

  1996cited by this paper
• On the directional specificity of ribosome frameshifting at a "hungry" codon.

  1993cited by this paper
• SEROLOGICAL EVIDENCE OF HERPESVIRUS INFECTION IN CAPTIVE ASIAN ELEPHANTS (ELEPHAS MAXIMUS)

  1990cited by this paper
• Acute and Fatal Herpesvirus Infection in a Young Asian Elephant (Elephas maximus)

  1990cited by this paper
• Cutaneous papillomas associated with a herpesvirus-like infection in a herd of captive African elephants.

  1986cited by this paper
• Herpes nodules in the lung of the African elephant (Loxodonta africana (Blumebach, 1792)).

  1971cited by this paper

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  2025cites this paper
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  2022cites this paper
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  2022cites this paper
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  2022cites this paper
• Development of an immunochromatographic strip test for antigen detection of elephant endotheliotropic herpesvirus in Asian elephants (Elephas maximus).

  2022cites this paper
• Development of Nonstructural Protein-Based Indirect ELISA to Identify Elephant Endotheliotropic Herpesvirus (EEHV) Infection in Asian Elephants (Elephas maximus)

  2022cites this paper
• Elephant Endotheliotropic Herpesvirus Impact in the European Asian Elephant (Elephas maximus) Population: Are Hereditability and Zoo-Associated Factors Linked with Mortality?

  2021cites this paper
• The occurrence of elephant endotheliotropic herpesvirus infection in wild and captive Asian elephants in Thailand: Investigation based on viral DNA and host antibody

  2021influential citation
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  2021cites this paper
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  2020cites this paper
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  2020influential citation
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  2020influential citation
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  2019cites this paper
• Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)

  2019cites this paper
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  2019cites this paper
• Elephant Endotheliotropic Herpesvirus

  2019cites this paper
• Review of the management of the Asian elephantElephas maximusEEP: current challenges and future solutions

  2019cites this paper
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  2018cites this paper
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  2018cites this paper
• Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants

  2018cites this paper
• Production of antibody against elephant endotheliotropic herpesvirus (EEHV) unveils tissue tropisms and routes of viral transmission in EEHV-infected Asian elephants

  2018cites this paper
• Evaluation of an In-house indirect ELISA for detection of antibody against haemorrhagic septicemia in Asian elephants.

  2017cites this paper
• Subclinical infection of a young captive Asian elephant with elephant endotheliotropic herpesvirus 1

  2017cites this paper
• Surviving and fatal Elephant Endotheliotropic Herpesvirus-1A infections in juvenile Asian elephants – lessons learned and recommendations on anti-herpesviral therapy

  2016cites this paper
• Distribution and load of elephant endotheliotropic herpesviruses in tissues from associated fatalities of Asian elephants.

  2016cites this paper