A Method for the Direct Identification of Differentiating Muscle Cells by a Fluorescent Mitochondrial Dye

Identification of differentiating muscle cells generally requires fixation, antibodies directed against muscle specific proteins, and lengthy staining processes or, alternatively, transfection of muscle specific reporter genes driving GFP expression. In this study, we examined the possibility of using the robust mitochondrial network seen in maturing muscle cells as a marker of cellular differentiation. The mitochondrial fluorescent tracking dye, MitoTracker, which is a cell-permeable, low toxicity, fluorescent dye, allowed us to distinguish and track living differentiating muscle cells visually by epi-fluorescence microscopy. MitoTracker staining provides a robust and simple detection strategy for living differentiating cells in culture without the need for fixation or biochemical processing.

• Publication year

  2011

• Venue

  PLoS ONE

• Publication date

  2011-12-09

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1371/journal.pone.0028628PMID 22174849PMCID 3235136
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• DUX4, a candidate gene for facioscapulohumeral muscular dystrophy, causes p53‐dependent myopathy in vivo

  2011cited by this paper
• Telomere dysfunction induces metabolic and mitochondrial compromise

  2011cited by this paper
• Mitochondrial fission and remodelling contributes to muscle atrophy

  2010cited by this paper
• Cardiotrophin-1 Maintains the Undifferentiated State in Skeletal Myoblasts*

  2009cited by this paper
• Nuclear Function of Smad7 Promotes Myogenesis

  2009cited by this paper
• Cyclosporin A corrects mitochondrial dysfunction and muscle apoptosis in patients with collagen VI myopathies

  2008cited by this paper
• Genetic and pharmacologic inhibition of mitochondrial-dependent necrosis attenuates muscular dystrophy

  2008cited by this paper
• Muscle cachexia is regulated by a p53-PW1/Peg3-dependent pathway.

  2006cited by this paper
• Cardiac Stem Cell and Myocyte Aging, Heart Failure, and Insulin-Like Growth Factor-1 Overexpression

  2004cited by this paper
• Mitochondrial dysfunction and apoptosis in myopathic mice with collagen VI deficiency

  2003cited by this paper
• TNFα inhibits skeletal myogenesis through a PW1‐dependent pathway by recruitment of caspase pathways

  2002cited by this paper
• Induction of Terminal Differentiation by the c-Jun Dimerization Protein JDP2 in C2 Myoblasts and Rhabdomyosarcoma Cells*

  2002cited by this paper
• A leucine‐rich nuclear export signal in the p53 tetramerization domain: regulation of subcellular localization and p53 activity by NES masking

  1999cited by this paper
• Cell heterogeneity upon myogenic differentiation: down-regulation of MyoD and Myf-5 generates 'reserve cells'.

  1998cited by this paper
• Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions

  1997cited by this paper
• Analysis of mitochondrial morphology and function with novel fixable fluorescent stains.

  1996cited by this paper
• Skeletal muscles of mice deficient in muscle creatine kinase lack burst activity.

  1993cited by this paper
• The MyoD DNA binding domain contains a recognition code for muscle-specific gene activation.

  1990cited by this paper
• MyoD and achaete-scute: 4-5 amino acids distinguishes myogenesis from neurogenesis.

  1989cited by this paper
• Influence of mitochondrial content on the sensitivity of respiratory control.

  1987cited by this paper
• Developmental progression of myosin gene expression in cultured muscle cells.

  1986cited by this paper
• Mitochondrial reticulum in limb skeletal muscle.

  1986cited by this paper
• Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle

  1977cited by this paper

• Animal-derived components in cultivated meat research and their alternatives

  2025cites this paper
• Areca nut extract exposure disrupts myogenesis and metabolism in C2C12 cells

  2025cites this paper
• Etomidate Induces Mitochondrial Dysfunction in Glioma Cancer Cells by Inhibiting Mitochondrial Biogenesis Mediated by CREB/PGC‐1α

  2025cites this paper
• Switching ubiquitous and muscle‐specific isoforms of mitochondrial respiratory complex IV in skeletal muscle fine‐tunes complex IV activity

  2023cites this paper
• Improved culture procedure for bovine muscle satellite cells for cultured meat.

  2023cites this paper
• Design and synthesis of photostable triphenylamine based neutral AIE nano luminogens: specific and long-term tracking of mitochondria in cells.

  2023cites this paper
• Notch1 and Notch2 Signaling Exclusively but Cooperatively Maintain Fetal Myogenic Progenitors.

  2022cites this paper
• Gain-of-Function STIM1 L96V Mutation Causes Myogenesis Alteration in Muscle Cells From a Patient Affected by Tubular Aggregate Myopathy

  2021cites this paper
• Identification of amino acid response element of SLC38A9 as an ATF4-binding site in porcine skeletal muscle cells.

  2021cites this paper
• Investigation of niclosamide as a repurposing agent for skeletal muscle atrophy

  2021cites this paper
• Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes

  2021cites this paper
• Principal protocols for the processing of cultured meat

  2021influential citation
• Sestrins are evolutionarily conserved mediators of exercise benefits

  2020cites this paper
• Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

  2020cites this paper
• Primary dermal fibroblasts and pectoralis muscle show similar patterns of oxidative stress in tropical and temperate birds despite differing life‐histories

  2020cites this paper
• Oxidative stress does not differ in primary dermal fibroblasts isolated from fast-growing and control-growing Japanese Quail (Coturnix japonica)

  2019cites this paper
• Ionic Pyridinium-Oxazole Dyads: Design, Synthesis, and Application in Mitochondrial Imaging.

  2019cites this paper
• Reversibly Assembled Electroconductive Hydrogel via a Host-Guest Interaction for 3D Cell Culture.

  2019cites this paper
• Actomyosin contractility scales with myoblast elongation and enhances differentiation through YAP nuclear export

  2019cites this paper
• conserved mediators exercise

  2019cites this paper
• Leucine, Palmitate, or Leucine/Palmitate Cotreatment Enhances Myotube Lipid Content and Oxidative Preference.

  2018cites this paper
• Cellular metabolic rates and oxidative stress profiles in primary fibroblast cells isolated from virgin females, reproductively experienced females, and male Sprague‐Dawley rats

  2018cites this paper
• Spatiotemporal magnetic fields enhance cytosolic Ca2+ levels and induce actin polymerization via activation of voltage-gated sodium channels in skeletal muscle cells.

  2018cites this paper
• Three-dimensional imaging reveals endo(sarco)plasmic reticulum-containing invaginations within the nucleoplasm of muscle.

  2018influential citation
• Sphingosine-1-phosphate pretreatment amends hypoxia-induced metabolic dysfunction and impairment of myogenic potential in differentiating C2C12 myoblasts by stimulating viability, calcium homeostasis and energy generation

  2018cites this paper
• A robust split-luciferase-based cell fusion screening for discovering myogenesis-promoting molecules.

  2018cites this paper
• Polymer microarrays for biomedical applications

  2017cites this paper
• CHROMIUM INHIBITS IN VITRO VIABILITY AND STEROIDOGENIC IN RAM LEYDIG CELLS

  2017cites this paper
• Metabolic effects of physiological levels of caffeine in myotubes

  2017cites this paper
• β-Cell deletion of Nr4a1 and Nr4a3 nuclear receptors impedes mitochondrial respiration and insulin secretion.

  2016cites this paper
• - Cell deletion of Nr 4 a 1 and Nr 4 a 3 nuclear receptors impedes mitochondrial respiration and insulin secretion

  2016influential citation
• Graphene Foam as a three-dimensional Platform for Myotube Growth.

  2016cites this paper
• Myotubes from lean and severely obese subjects with and without type 2 diabetes respond differently to an in vitro model of exercise.

  2015cites this paper
• Specific imaging and tracking of mitochondria in live cells by a photostable AIE luminogen.

  2015cites this paper
• IL-15 Signaling in Skeletal Muscle: Implications in Modulation of PGC-1alpha and Mitochondrial Biogenesis

  2014cites this paper
• A photostable AIE luminogen for specific mitochondrial imaging and tracking.

  2013cites this paper
• Calorie restriction in overweight males ameliorates obesity-related metabolic alterations and cellular adaptations through anti-aging effects, possibly including AMPK and SIRT1 activation.

  2013cites this paper
• Low Glucose but Not Galactose Enhances Oxidative Mitochondrial Metabolism in C2C12 Myoblasts and Myotubes

  2013cites this paper
• “Stainomics”: Identification of mitotracker labeled proteins in mammalian cells

  2013cites this paper
• Selective mitochondrial staining with small fluorescent probes: importance, design, synthesis, challenges and trends for new markers

  2013cites this paper
• Live Cell Imaging Methods Review

  2012cites this paper
• Thimerosal-Induced Apoptosis in Mouse C2C12 Myoblast Cells Occurs through Suppression of the PI3K/Akt/Survivin Pathway

  2012cites this paper