MicroRNA-195 regulates proliferation, migration, angiogenesis and autophagy of endothelial progenitor cells by targeting GABARAPL1

Deep vein thrombosis (DVT) is a common type of venous thrombosis. Successful resolution of DVT-related thrombi is important in the treatment of DVT. Endothelial progenitor cells (EPCs) have emerged as a promising therapeutic choice for DVT-related thrombus resolution; however, the clinical application of EPCs faces many challenges. In the present study, the expression of miR-582, miR-195 and miR-532 under hypoxic or normoxic conditions was measured using quantitative real-time PCR analysis (qRT-PCR) and the results showed that the increased fold of miR-195 was highest in human EPCs (hEPCs) under hypoxic conditions. Then the role and regulating mechanism of miR-195 in improving the function of EPCs was investigated. To investigate the effect of miR-195 inhibition on the autophagy of hEPCs, the expression of the autophagy-related genes LC3B and beclin1 was examined using western blotting, and the formation of autophagosomes was observed using TEM. The results indicated that the inhibition of miR-195 expression could promote autophagy of hEPCs. In addition, we investigated the role of miR-195 on the proliferation, migration and angiogenesis of hEPCs under hypoxia. The results revealed that miR-195 inhibition promotes cell proliferation, migration and angiogenesis of hEPCs under hypoxia. Furthermore, GABA type A receptor associated protein like 1 (GABARAPL1) was identified as a directed target of miR-195 and GABARAPL1 silencing could decrease the effect of miR-195 knockdown on cell proliferation, migration, angiogenesis and autophagy of hEPCs under hypoxia. Together, these results indicate that miR-195 regulates cell proliferation, migration, angiogenesis and autophagy of hEPCs by targeting GABARAPL1.

• Publication year

  2016

• Venue

  Bioscience Reports

• Publication date

  2016-09-13

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1042/BSR20160139PMID 27623937PMCID 5064457
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Plasma from pre‐eclamptic patients induces the expression of the anti‐angiogenic miR‐195‐5p in endothelial cells

  2016cited by this paper
• Endothelial progenitor cells accelerate the resolution of deep vein thrombosis.

  2016cited by this paper
• The NF-κB-modulated microRNAs miR-195 and miR-497 inhibit myoblast proliferation by targeting Igf1r, Insr and cyclin genes

  2016cited by this paper
• MicroRNA-195a-5p inhibits mouse medullary thymic epithelial cells proliferation by directly targeting Smad7.

  2016cited by this paper
• Transcription factor E2-2 inhibits the proliferation of endothelial progenitor cells by suppressing autophagy

  2016cited by this paper
• RNF216 contributes to proliferation and migration of colorectal cancer via suppressing BECN1-dependent autophagy

  2016cited by this paper
• MiR-195 Suppresses Cervical Cancer Migration and Invasion Through Targeting Smad3

  2016cited by this paper
• Roles of MicroRNA-195 in cardiomyocyte apoptosis induced by myocardial ischemia-reperfusion injury.

  2016cited by this paper
• Role of Autophagy in Hypoxia-Induced Angiogenesis of RF/6A Cells in vitro

  2016cited by this paper
• Hypoxia induces the proliferation of endothelial progenitor cells via upregulation of Apelin/APLNR/MAPK signaling.

  2016cited by this paper
• Upregulation of MicroRNA‐126 Contributes to Endothelial Progenitor Cell Function in Deep Vein Thrombosis via Its Target PIK3R2

  2015cited by this paper
• Are Endothelial Progenitor Cells the Real Solution for Cardiovascular Diseases? Focus on Controversies and Perspectives

  2015cited by this paper
• MicroRNA-195 inhibits proliferation, invasion and metastasis in breast cancer cells by targeting FASN, HMGCR, ACACA and CYP27B1

  2015cited by this paper
• MicroRNA-195 induced apoptosis in hypoxic chondrocytes by targeting hypoxia-inducible factor 1 alpha.

  2015cited by this paper
• miR-195 in human primary mesenchymal stromal/stem cells regulates proliferation, osteogenesis and paracrine effect on angiogenesis

  2015cited by this paper
• MiR-145 facilitates proliferation and migration of endothelial progenitor cells and recanalization of arterial thrombosis in cerebral infarction mice via JNK signal pathway.

  2015cited by this paper
• Transplantation of bradykinin-preconditioned human endothelial progenitor cells improves cardiac function via enhanced Akt/eNOS phosphorylation and angiogenesis.

  2015cited by this paper
• A panel of microRNAs as a new biomarkers for the detection of deep vein thrombosis

  2015cited by this paper
• Silencing of miR-195 reduces diabetic cardiomyopathy in C57BL/6 mice

  2015cited by this paper
• MiR-150 enhances the motility of EPCs in vitro and promotes EPCs homing and thrombus resolving in vivo.

  2014cited by this paper
• Increased miR‐195 aggravates neuropathic pain by inhibiting autophagy following peripheral nerve injury

  2013cited by this paper
• Autophagy as a stress-response and quality-control mechanism: implications for cell injury and human disease.

  2013cited by this paper
• MicroRNA‐195 Suppresses Angiogenesis and Metastasis of Hepatocellular Carcinoma by Inhibiting the Expression of VEGF, VAV2, and CDC42

  2013cited by this paper
• Adverse effects of heparin.

  2012cited by this paper
• Heparin - A Century of Progress

  2012cited by this paper
• Cytoprotective roles for autophagy

  2010cited by this paper
• GABARAPL1 (GEC1) associates with autophagic vesicles

  2010cited by this paper
• Vascular repair by endothelial progenitor cells.

  2008cited by this paper
• Bleeding complications with warfarin use: a prevalent adverse effect resulting in regulatory action.

  2007cited by this paper
• Endothelial progenitor cells as a new agent contributing to vascular repair

  2007cited by this paper
• The role of neovascularisation in the resolution of venous thrombus

  2005cited by this paper
• The functions of animal microRNAs

  2004cited by this paper
• Intensified Cloning Efforts Have Revealed Numer

  2004cited by this paper
• Oxygen: aspects of its toxicity and elements of defense.

  1984cited by this paper

• Role of autophagy response on angiogenesis activity of endothelial progenitor cells.

  2025cites this paper
• Advances in microRNA regulation of deep vein thrombosis through venous vascular endothelial cells (Review).

  2024cites this paper
• Non-coding RNAs regulating endothelial progenitor cells for venous thrombosis: promising therapy and innovation

  2024cites this paper
• Analysis and identification of ferroptosis-related diagnostic markers in rheumatoid arthritis

  2024cites this paper
• HOXD9 regulated mitophagy to promote endothelial progenitor cells angiogenesis and deep vein thrombosis recanalization and resolution

  2024cites this paper
• The emerging roles of miRNA-mediated autophagy in ovarian cancer

  2024cites this paper
• Matrine Protects Endothelial Progenitor Cells against Apoptosis and Promotes Their Migration, Invasion, and Tube Formation Abilities via Modulating miR-126b/FOXO4 Axis

  2023cites this paper
• The role of PIWI-interacting RNA in naringin pro-angiogenesis by targeting HUVECs.

  2023cites this paper
• Downregulation of miR-125a-5p Promotes Endothelial Progenitor Cell Migration and Angiogenesis and Alleviates Deep Vein Thrombosis in Mice Via Upregulation of MCL-1

  2023cites this paper
• Long noncoding RNA TUG1 induces angiogenesis of endothelial progenitor cells and dissolution of deep vein thrombosis

  2022cites this paper
• MicroRNA miR-378-3p is a novel regulator of endothelial autophagy and function

  2022cites this paper
• MicroRNA-136-5p from Endothelial Progenitor Cells-released Extracellular Vesicles Mediates TXNIP to Promote the Dissolution of Deep Venous Thrombosis

  2022cites this paper
• Combination of circulating miR-125a-5p, miR-223-3p and D-dimer as a novel biomarker for Deep Vein Thrombosis.

  2022cites this paper
• Upregulated miR-206 Aggravates Deep Vein Thrombosis by Regulating GJA1-Mediated Autophagy of Endothelial Progenitor Cells

  2022cites this paper
• The role of miRNAs in regulation of platelet activity and related diseases - a bioinformatic analysis

  2022cites this paper
• Integrated bioinformatics analysis identifies microRNA-200a-5p as a new plasma marker in patient with venous thromboembolism.

  2022cites this paper
• Circulating MicroRNAs for Diagnosis of Acute Pulmonary Embolism: Still a Long Way to Go

  2022cites this paper
• The Emerging Roles of Autophagy-Related MicroRNAs in Cancer

  2021cites this paper
• Curcumin promotes venous thrombi resolve process in a mouse deep venous thrombosis model via regulating miR-499.

  2021cites this paper
• LncRNA UCA1, miR‐26a, and miR‐195 in coronary heart disease patients: Correlation with stenosis degree, cholesterol levels, inflammatory cytokines, and cell adhesion molecules

  2021cites this paper
• Identification and Validation of Potential Biomarkers and Pathways for Idiopathic Pulmonary Fibrosis by Comprehensive Bioinformatics Analysis

  2021cites this paper
• MiRNA 126 as a New Predictor Biomarker in Venous Thromboembolism of Persistent Residual Vein Obstruction: A Review of the Literature Plus a Pilot Study

  2021cites this paper
• MicroRNAs Contribute to Metastasis by Regulating Autophagy: Recent Concepts

  2021cites this paper
• miR‐195 reduces age‐related blood–brain barrier leakage caused by thrombospondin‐1‐mediated selective autophagy

  2020cites this paper
• Cerebrovascular miRNAs correlate with the clearance of Aβ through perivascular route in younger 3xTg‐AD mice

  2020cites this paper
• MicroRNAs as major regulators of the autophagy pathway.

  2020cites this paper
• MicroRNAs in Venous Thrombo-Embolism.

  2020cites this paper
• Oxidative stress‐induced RAC autophagy can improve the HUVEC functions by releasing exosomes

  2020cites this paper
• LncRNA GUSBP5-AS promotes EPC migration and angiogenesis and deep vein thrombosis resolution by regulating FGF2 and MMP2/9 through the miR-223-3p/FOXO1/Akt pathway

  2020cites this paper
• Retracted: Silencing of Exosomal miR‐296‐5p Derived from Endothelial Progenitor Cells Enhances Thrombolysis and Recanalization in Deep Vein Thrombosis

  2020cites this paper
• Role of microRNAs in Hemophilia and Thrombosis in Humans

  2020cites this paper
• Identification of feature autophagy-related genes in patients with acute myocardial infarction based on bioinformatics analyses

  2020cites this paper
• Down-regulation of miR-361-5p promotes the viability, migration and tube formation of endothelial progenitor cells via targeting FGF1

  2020cites this paper
• Clarification of the Role of miR-9 in the Angiogenesis, Migration, and Autophagy of Endothelial Progenitor Cells Through RNA Sequence Analysis

  2020cites this paper
• miRNAs and Its Regulatory Role on Autophagy in Tumor Microenvironment

  2020cites this paper
• Construction of a model to predict the prognosis of patients with cholangiocarcinoma using alternative splicing events

  2019cites this paper
• MiR‐205 promotes endothelial progenitor cell angiogenesis and deep vein thrombosis recanalization and resolution by targeting PTEN to regulate Akt/autophagy pathway and MMP2 expression

  2019cites this paper
• IL (Interleukin)-6 Contributes to Deep Vein Thrombosis and Is Negatively Regulated by miR-338-5p

  2019cites this paper
• MicroRNAs Involved in the Regulation of Angiogenesis in Bone Regeneration

  2019cites this paper
• Modulated Autophagy by MicroRNAs in Osteoarthritis Chondrocytes

  2019cites this paper
• siRNA‐mediated silencing of PAI‐1 gene acts as a promoter over the recanalization of endothelial progenitor cells in rats with venous thrombosis

  2019cites this paper
• Upregulated MiR-9-5p Protects Against Inflammatory Response in Rats with Deep Vein Thrombosis via Inhibition of NF-κB p50

  2019cites this paper
• MicroRNA-200c promotes tumor cell proliferation and migration by directly targeting dachshund family transcription factor 1 by the Wnt/&bgr;-catenin signaling pathway in nasopharyngeal carcinoma

  2019cites this paper
• Myocardial Energy Stress, Autophagy Induction, and Cardiomyocyte Functional Responses

  2019cites this paper
• Interaction among inflammasome, autophagy and non-coding RNAs: new horizons for drug

  2019cites this paper
• Potential ceRNA networks involved in autophagy suppression of pancreatic cancer caused by chloroquine diphosphate: A study based on differentially-expressed circRNAs, lncRNAs, miRNAs and mRNAs

  2018cites this paper
• BIOINFORMATIC ANALYSIS OF DOSE- AND TIME-DEPENDENT miRNome RESPONSES.

  2018cites this paper
• From Oxidative Stress Damage to Pathways, Networks, and Autophagy via MicroRNAs

  2018cites this paper
• Human cancer cells suppress behaviors of endothelial progenitor cells through miR-21 targeting IL6R.

  2018cites this paper
• Characterization of microRNA expression in primary human colon adenocarcinoma cells (SW480) and their lymph node metastatic derivatives (SW620)

  2018cites this paper
• HSF1 deficiency accelerates the transition from pressure overload-induced cardiac hypertrophy to heart failure through endothelial miR-195a-3p-mediated impairment of cardiac angiogenesis.

  2018cites this paper
• The regulatory role of microRNAs in angiogenesis‐related diseases

  2018cites this paper
• The intragenic mRNA-microRNA regulatory network during telogen-anagen hair follicle transition in the cashmere goat

  2018cites this paper
• Silence of lncRNA UCA1 Represses the Growth and Tube Formation of Human Microvascular Endothelial Cells Through miR-195

  2018cites this paper
• Role of Autophagy in HIV-1 Matrix Protein p17-Driven Lymphangiogenesis

  2017cites this paper
• Circulating microRNA expression and their target genes in deep vein thrombosis

  2017cites this paper
• mRNA-miRNA integrative analysis of diabetes-induced cardiomyopathy in rats.

  2017cites this paper
• The zebrafish miR-125c is induced under hypoxic stress via hypoxia-inducible factor 1α and functions in cellular adaptations and embryogenesis

  2017cites this paper
• Autophagy-Regulating microRNAs and Cancer

  2017cites this paper
• Functions of MicroRNAs in Angiogenesis

  2017cites this paper
• Myocardial stress and autophagy: mechanisms and potential therapies

  2017cites this paper
• Downregulation of microRNA-195 promotes angiogenesis induced by cerebral infarction via targeting VEGFA

  2017cites this paper
• MicroRNA-532 protects the heart in acute myocardial infarction, and represses prss23, a positive regulator of endothelial-to-mesenchymal transition

  2017cites this paper