Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein

A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum α-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA). A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents, less total assay time, and better linearity, recovery, precision, sensitivity and validity. AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA, and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) kit. The correlation coefficient between MPs-CLEIA and ELISA was obtained with R2=0.6703; however, the correlation between MPs-CLEIA and ECLIA (R2=0.9582) was obviously better than that between colorimetric ELISA and ECLIA (R2=0.6866).

• Publication year

  2012

• Venue

  Journal of Pharmaceutical Analysis

• Publication date

  2012-01-27

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.jpha.2011.10.001PMID 29403732PMCID 5760820
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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