Substrate specificity effects of lipoxygenase products and inhibitors on soybean lipoxygenase-1.

Recently, it has been shown that lipoxygenase (LO) products affect the substrate specificity of human 15-LO. In the current paper, we demonstrate that soybean LO-1 (sLO-1) is not affected by its own products, however, inhibitors which bind the allosteric site, oleyl sulfate (OS) and palmitoleyl sulfate (PS), not only lower catalytic activity, but also change the substrate specificity, by increasing the arachidonic acid (AA)/linoleic acid (LA) ratio to 4.8 and 4.0, respectively. The fact that LO inhibitors can lower activity and also change the LO product ratio is a new concept in lipoxygenase inhibition, where the goal is to not only reduce the catalytic activity but also alter substrate selectivity towards a physiologically beneficial product.

• Publication year

  2009

• Venue

  Bioorganic & Medicinal Chemistry

• Publication date

  2009-09-15

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.bmc.2009.08.005PMID 19716306PMCID PMC2737062
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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