Resveratrol increases BRCA1 and BRCA2 mRNA expression in breast tumour cell lines

The phytochemical resveratrol, found in grapes, berries and peanuts, has been found to possess cancer chemopreventive effects by inhibiting diverse cellular events associated with tumour initiation, promotion and progression. Resveratrol is also a phyto-oestrogen, binds to and activates oestrogen receptors that regulate the transcription of oestrogen-responsive target genes such as the breast cancer susceptibility genes BRCA1 and BRCA2. We investigated the effects of resveratrol on BRCA1 and BRCA2 expression in human breast cancer cell lines (MCF7, HBL 100 and MDA-MB 231) using quantitative real-time RT–PCR, and by perfusion chromatography of the proteins. All cell lines were treated with 30 μM resveratrol. The expressions of BRCA1 and BRCA2 mRNAs were increased although no change in the expression of the proteins were found. These data indicate that resveratrol at 30 μM can increase expression of genes involved in the aggressiveness of human breast tumour cell lines.

• Publication year

  2003

• Venue

  British Journal of Cancer

• Publication date

  2003-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/sj.bjc.6600983PMID 12838319PMCID 2394230
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Resveratrol acts as a mixed agonist/antagonist for estrogen receptors alpha and beta.

  2000influential reference
• Isolation, purification and quantification of BRCA1 protein from tumour cells by affinity perfusion chromatography.

  1999cited by this paper
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  1999cited by this paper
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  1999cited by this paper
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  1999cited by this paper
• Antiproliferative effect of synthetic resveratrol on human breast epithelial cells.

  1998cited by this paper
• DNA polymerase and topoisomerase II inhibitors from Psoralea corylifolia.

  1998cited by this paper
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  1998cited by this paper
• Real-time quantitative RT–PCR after laser-assisted cell picking

  1998cited by this paper
• Resveratrol arrests the cell division cycle at S/G2 phase transition.

  1998cited by this paper
• Estrogen upregulation of BRCA1 expression with no effect on localization

  1998influential reference
• Differential subcellular localization, expression and biological toxicity of BRCA1 and the splice variant BRCA1-delta11b.

  1997cited by this paper
• Resveratrol, a polyphenolic compound found in grapes and wine, is an agonist for the estrogen receptor.

  1997influential reference
• Life and death in the cytoplasm: messages from the 3' end.

  1997cited by this paper
• Cell cycle-dependent colocalization of BARD1 and BRCA1 proteins in discrete nuclear domains.

  1997cited by this paper
• Differential subcellular localization, expression and biological toxicity of BRCA1 and the splice variant BRCA1-Δ11b

  1997cited by this paper
• Cancer Chemopreventive Activity of Resveratrol, a Natural Product Derived from Grapes

  1997cited by this paper
• Complex Regulation of the BRCA1 Gene*

  1997cited by this paper
• Localization of BRCA1 and a splice variant identifies the nuclear localization signal

  1997influential reference
• Methylation of the BRCA1 gene in sporadic breast cancer.

  1997cited by this paper
• Nuclear location and cell cycle regulation of the BRCA2 protein.

  1997cited by this paper
• Cell cycle regulation of BRCA1 messenger RNA in human breast epithelial cells.

  1996cited by this paper
• Somatic and germline mutations of the BRCA2 gene in sporadic ovarian cancer.

  1996cited by this paper
• BRCA1 and BRCA2 mRNA levels are coordinately elevated in human breast cancer cells in response to estrogen.

  1996cited by this paper
• Cell cycle control of BRCA2.

  1996cited by this paper
• Translational regulation: versatile mechanisms for metabolic and developmental control.

  1995cited by this paper
• Hormone-dependent regulation of BRCA1 in human breast cancer cells.

  1995cited by this paper
• A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1.

  1994cited by this paper
• Angiogenesis and neoplastic progression in vitro.

  1982cited by this paper
• Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining

  1975cited by this paper
• Tissue culture studies on pleural effusions from breast carcinoma patients.

  1974cited by this paper
• A human cell line from a pleural effusion derived from a breast carcinoma.

  1973cited by this paper
• Printed in U.S.A. Copyright © 2000 by The Endocrine Society Resveratrol Acts as a Mixed Agonist/Antagonist for

  year unknowninfluential reference

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  2017cites this paper
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