Physiological effects of gasoline on the freshwater fish Prochilodus lineatus(Characiformes: Prochilodontidae)

The purpose of this work was to evaluate the effects of the water-soluble fraction of gasoline (WSFG) on the Neotropical freshwater fish Prochilodus lineatus. The WSFG was prepared by mixing gasoline in water (1:4) and animals were exposed for 6, 24 and 96h to 5% diluted WSFG or only to water. After exposure, blood was collected from the caudal vein and the gills were removed. The following parameters were analyzed: hematological (hemoglobin, hematocrit, number of red blood cells), osmo-ionic (plasma Na+, Cl- and K+ and plasma osmolarity), metabolic (total plasma proteins and glucose), endocrine (cortisol), density and distribution of chloride cells [CC] in the gills (immunohistochemistry), and branchial Na+/K+-ATPase (NKA) activity. Hemolysis was found to occur after 96h exposure to WSFG, as indicated by the decrease in the hematological parameters analyzed, followed by an increase in plasma K+. Secondary stress response was revealed by the occurrence of hyperglycemia in the three periods of exposure, despite the absence of significant increases in the plasma cortisol. The exposure to WSFG also caused an increase in the quantity of CC and in plasma Na+, after 24h, as well as in the enzymatic activity of NKA and plasma osmolarity, after 24h and 96h. These results indicate that fish exposed to the WSFG showed physiological adjusts to maintain their osmotic balance. However, the increase in the quantity of CC in the lamellae may interfere in the gas exchange impairing respiration.

• Publication year

  2013

• Venue

  Neotropical Ichthyology

• Publication date

  2013-09-01

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.1590/S1679-62252013000300022
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Hematological and biochemical alterations in the fish Prochilodus lineatus caused by the herbicide clomazone.

  2013cited by this paper
• Atrazine promotes biochemical changes and DNA damage in a Neotropical fish species.

  2012cited by this paper
• Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

  2012cited by this paper
• Fish thyroidal and stress responses in contamination monitoring--an integrated biomarker approach.

  2011cited by this paper
• Gasoline effects on biotransformation and antioxidant defenses of the freshwater fish Prochilodus lineatus

  2011cited by this paper
• Sodium Fluxes in Tamoatá, Hoplosternum litoralle, Exposed to Formation Water from Urucu Reserve (Amazon, Brazil)

  2011cited by this paper
• Acute effects of chemically dispersed crude oil on gill ion regulation, plasma ion levels and haematological parameters in tambaqui (Colossoma macropomum).

  2010cited by this paper
• Roundup causes oxidative stress in liver and inhibits acetylcholinesterase in muscle and brain of the fish Prochilodus lineatus.

  2010cited by this paper
• How aluminium exposure promotes osmoregulatory disturbances in the neotropical freshwater fish Prochilus lineatus.

  2009cited by this paper
• Multiple biomarkers responses in Prochilodus lineatus allowed assessing changes in the water quality of Salado River basin (Santa Fe, Argentina).

  2009cited by this paper
• Organ specific antioxidant responses in golden grey mullet (Liza aurata) following a short-term exposure to phenanthrene.

  2008cited by this paper
• Biochemical, physiological, and histological changes in the neotropical fish Prochilodus lineatus exposed to diesel oil.

  2008cited by this paper
• Naphthalene treatment alters liver intermediary metabolism and levels of steroid hormones in plasma of rainbow trout (Oncorhynchus mykiss).

  2007cited by this paper
• New insights into fish ion regulation and mitochondrion-rich cells.

  2007cited by this paper
• Aryl hydrocarbon receptor activation impairs cortisol response to stress in rainbow trout by disrupting the rate-limiting steps in steroidogenesis.

  2006cited by this paper
• Effects of the Water Soluble Fraction of Diesel Fuel Oil on Some Functional Parameters of the Neotropical Freshwater Fish Prochilodus Lineatus Valenciennes

  2006cited by this paper
• Effects of acute and prolonged naphthalene exposure on brain monoaminergic neurotransmitters in rainbow trout (Oncorhynchus mykiss).

  2006cited by this paper
• Sparus aurata L. liver EROD and GST activities, plasma cortisol, lactate, glucose and erythrocytic nuclear anomalies following short-term exposure either to 17beta-estradiol (E2) or E2 combined with 4-nonylphenol.

  2005cited by this paper
• Contaminação de águas por BTXs e processos utilizados na remediação de sítios contaminados

  2004cited by this paper
• Molecular biology of major components of chloride cells.

  2003cited by this paper
• Naphthalene-induced differential tissue damage association with circulating fish phagocyte induction.

  2003cited by this paper
• Acid-base regulation in fishes: cellular and molecular mechanisms.

  2002cited by this paper
• Biotransformation, endocrine, and genetic responses of Anguilla anguilla L. to petroleum distillate products and environmentally contaminated waters.

  2001cited by this paper
• Evidence for a mineralocorticoid-like receptor linked to branchial chloride cell proliferation in freshwater rainbow trout.

  2001cited by this paper
• Epithelial gill cells in the armored catfish, Hypostomus cf. plecostomus (Loricariidae).

  2001cited by this paper
• Na(+)/K(+)-ATPase immunoreactivity in branchial chloride cells of Oreochromis mossambicus exposed to copper.

  2000cited by this paper
• Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation

  1999cited by this paper
• The role of circulating catecholamines in the regulation of fish metabolism: an overview.

  1998cited by this paper
• Gill morphology and acid-base regulation in freshwater fishes.

  1998influential reference
• Cortisol protects against copper induced necrosis and promotes apoptosis in fish gill chloride cells in vitro

  1998cited by this paper
• Photochemistry of petroleum: I. Systematic study of a brazilian intermediate crude oil

  1998cited by this paper
• Handling stress does not affect the expression of hepatic heat shock protein 70 and conjugation enzymes in rainbow trout treated with beta-naphthoflavone.

  1997influential reference
• The stress response in fish

  1997influential reference
• The chloride cell: structure and function in the gills of freshwater fishes.

  1997cited by this paper
• Interrenal stress responsiveness of tilapia (Oreochromis mossambicus) is impaired by dietary exposure to PCB 126.

  1997cited by this paper
• Endocrine, osmoregulatory, respiratory and haematological parameters in flounder exposed to the water soluble fraction of crude oil

  1996cited by this paper
• THE EFFECTS OF BRANCHIAL CHLORIDE CELL PROLIFERATION ON RESPIRATORY FUNCTION IN THE RAINBOW TROUT ONCORHYNCHUS MYKISS

  1994influential reference
• The effects of chronic exposure to the water soluble fraction of fuel oil on an antarctic fish Pagothenia borchgkevinki

  1993cited by this paper
• Mechanisms of hormone actions on gill transport

  1987cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper

• Effect of Food Deprivation on Plasma Cortisol, Carbohydrate Metabolism, and Histomorphology in Clarias batrachus

  2025cites this paper
• Multi-omics reveal L-DOPA-mediated growth inhibition in turbot (Scophthalmus maximus): Overlooked metabolites for water quality management in recirculating aquaculture systems

  2025cites this paper
• Prefeeding of Clarias gariepinus with Spirulina platensis counteracts petroleum hydrocarbons-induced hepato- and nephrotoxicity

  2024cites this paper
• Potential role of dietary Boswellia serrata resin against mancozeb fungicide-induced immune-antioxidant suppression, histopathological alterations, and genotoxicity in Nile tilapia, Oreochromis niloticus.

  2023cites this paper
• Oxidative Stress and Immunopathological Alterations of Clarias gariepinus Exposed to Monocyclic Aromatic Hydrocarbons (BTX)

  2023cites this paper
• Effects of prolonged fasting on levels of metabolites, oxidative stress, immune-related gene expression, histopathology, and DNA damage in the liver and muscle tissues of rainbow trout (Oncorhynchus mykiss)

  2021cites this paper
• Combine effect of exposure to petrol, kerosene and diesel fumes: On hepatic oxidative stress and haematological function in rats

  2021cites this paper
• Effect of petroleum wastewater treated with gravity separation and magnetite nanoparticles adsorption methods on the blood biochemical response of mrigal fish (Cirrhinus cirrhosus)

  2021cites this paper
• Chemical exposure hazardous for fish Hyphessobrycon eques through the incorrect release of oil in Amazon region

  2020influential citation
• Breathing and respiratory adaptations

  2020cites this paper
• Short-term mercury exposure in tilapia (Oreochromis niloticus) at different salinities: impact on serum osmoregulation, hematological parameters, and Na+/K+-ATPase level

  2020cites this paper
• Fire Suppression Agents Combined with Gasoline in Aquatic Ecosystems: A Mixture Approach

  2020cites this paper
• A comparative study on the effects of selected pesticides on hemato-biochemistry and tissue histology of freshwater fish Cirrhinus mrigala (Hamilton, 1822)

  2020cites this paper
• Alterações leucocitárias ocasionadas pelo estresse toxicológico agudo do óleo diesel em juvenis de tilápia-do-nilo

  2018influential citation
• Effect of Cd on serum osmolality, ion levels and hematological parameters of tilapia (Oreochromis niloticus) at different salinity levels

  2018cites this paper
• Stress indicators for Prochilodus lineatus (Characiformes: Prochilodontidae) breeders during passage through a fish ladder

  2018cites this paper
• Physio-metabolic response of rainbow trout during prolonged food deprivation before slaughter

  2018cites this paper
• Effect of sub-lethal lead exposure at different salinities on osmoregulation and hematological changes in tilapia, Oreochromis niloticus

  2017cites this paper
• Effects of Petrol Exposure on Glucose, Liver and Muscle glycogen levels in the Common African toad Bufo regularis.

  2017cites this paper
• Water-soluble fraction of petroleum induces genotoxicity and morphological effects in fat snook (Centropomus parallelus).

  2017cites this paper