Vectors for N- or C-terminal positioning of the yeast Gal4p DNA binding or activator domains.

No abstract is available for this paper.

• Publication year

  2003

• Venue

  BioTechniques

• Publication date

  2003-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.2144/03351BM06PMID 12866406
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Yeast is selectively hypersensitised to heat shock protein 90 (Hsp90)-targetting drugs with heterologous expression of the human Hsp90beta, a property that can be exploited in screens for new Hsp90 chaperone inhibitors.

  2003cited by this paper
• Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1.

  2002cited by this paper
• Heat-shock protein 90, a chaperone for folding and regulation

  2002cited by this paper
• A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae

  2000cited by this paper
• Structure and in vivo function of Hsp90.

  2000cited by this paper
• The ATPase cycle of Hsp90 drives a molecular ‘clamp’ via transient dimerization of the N‐terminal domains

  2000cited by this paper
• ATP binding and hydrolysis are essential to the function of the Hsp90 molecular chaperone in vivo

  1998cited by this paper
• Getting more from the two-hybrid system: N-terminal fusions to LexA are efficient and sensitive baits for two-hybrid studies.

  1997cited by this paper
• Conservation of Hsp90 macromolecular complexes in Saccharomyces cerevisiae.

  1994cited by this paper

• A split GAL4 RUBY assay for visual in planta detection of protein-protein interactions.

  2023cites this paper
• Hsp90 and phosphorylation of the Slt2(Mpk1) MAP kinase activation loop are essential for catalytic, but not non-catalytic, Slt2-mediated transcription in yeast

  2022cites this paper
• Inhibition of Arabidopsis stomatal development by plastoquinone oxidation.

  2021cites this paper
• The human Shu complex functions with PDS5B and SPIDR to promote homologous recombination

  2019cites this paper
• Budding yeast CENP-ACse4 interacts with the N-terminus of Sgo1 and regulates its association with centromeric chromatin

  2018cites this paper
• PipY, a Member of the Conserved COG0325 Family of PLP-Binding Proteins, Expands the Cyanobacterial Nitrogen Regulatory Network

  2017cites this paper
• Pnc1 piggy-back import into peroxisomes relies on Gpd1 homodimerisation

  2017cites this paper
• Mutation of the Ser18 phosphorylation site on the sole Saccharomyces cerevisiae UCS protein, She4, can compromise high-temperature survival

  2016cites this paper
• Investigation of Post-Translational Modifications in Staphylococcus aureus

  2015cites this paper
• Cdc37 engages in stable, S14A mutation-reinforced association with the most atypical member of the yeast kinome, Cdk-activating kinase (Cak1)

  2014cites this paper
• Investigation of Hsf1 Interacting Partners via a Genome-wide Yeast Two-hybrid Screen

  2013cites this paper
• Essential ribosome assembly factor Fap7 regulates a hierarchy of RNA–protein interactions during small ribosomal subunit biogenesis

  2013cites this paper
• The amino terminal end determines the stability and assembling capacity of eukaryotic ribosomal stalk proteins P1 and P2

  2011cites this paper
• Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome

  2010cites this paper
• In vivo formation of a stable pentameric (P2α/P1β)–P0–(P1α/P2β) ribosomal stalk complex in Saccharomyces cerevisiae

  2010influential citation
• Nbl1p: a Borealin/Dasra/CSC-1-like protein essential for Aurora/Ipl1 complex function and integrity in Saccharomyces cerevisiae.

  2009cites this paper
• Benchmarking yeast two‐hybrid systems using the interactions of bacterial motility proteins

  2009cites this paper
• Structural and functional characterization of the amino terminal domain of the yeast ribosomal stalk P1 and P2 proteins.

  2009cites this paper
• Regulation of mitogen-activated protein kinase signaling by the molecular chaperones Hsp90 and Cdc37

  2008cites this paper
• Yeast Arf3p Modulates Plasma Membrane PtdIns(4,5)P2 Levels to Facilitate Endocytosis

  2008cites this paper
• Phosphorylation-independent activation of the atypical response regulator NblR.

  2008cites this paper
• Expressed as the sole Hsp90 of yeast, the alpha and beta isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90beta generates sensitivity to the Hsp90 inhibitor radicicol.

  2007cites this paper
• Hog1 Mitogen-Activated Protein Kinase Phosphorylation Targets the Yeast Fps1 Aquaglyceroporin for Endocytosis, Thereby Rendering Cells Resistant to Acetic Acid

  2007cites this paper
• A protein interaction map of the mitotic spindle.

  2007cites this paper
• Molecular interaction network of the Hsp90 chaperone system.

  2007cites this paper
• Molecular aspects of the stress response : chaperones, membranes and networks

  2007cites this paper
• Expressed as the sole Hsp90 of yeast, the α and β isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90β generates sensitivity to the Hsp90 inhibitor radicicol

  2007cites this paper
• Regulation of budding yeast kinetochore proteins by SUMO modification

  2007cites this paper
• Expressed in yeast , human ERK 5 is a client of the Hsp 90 chaperone that complements loss of the Slt 2 ( Mpk 1 ) p cell integrity stress-activated protein kinase

  2006cites this paper
• Expressed in the Yeast Saccharomyces cerevisiae, Human ERK5 Is a Client of the Hsp90 Chaperone That Complements Loss of the Slt2p (Mpk1p) Cell Integrity Stress-Activated Protein Kinase

  2006cites this paper
• Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation

  2006cites this paper
• Functional analysis of S.pombe Cdc37

  2005cites this paper
• Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex

  2005cites this paper
• A Two-Hybrid Screen of the Yeast Proteome for Hsp90 Interactors Uncovers a Novel Hsp90 Chaperone Requirement in the Activity of a Stress-Activated Mitogen-Activated Protein Kinase, Slt2p (Mpk1p)

  2005cites this paper
• Investigating the protein-protein interactions of the yeast Hsp90 chaperone system by two-hybrid analysis: potential uses and limitations of this approach

  2004cites this paper
• Jcb: Article Introduction

  2002cites this paper
• Sumoylation of The Budding Yeast Kinetochore Protein Ndc10 is Sumoylation of The Budding Yeast Kinetochore Protein Ndc10 is Required for Ndc10 Spindle Localization and Regulation of Required for Ndc10 Spindle Localization and Regulation of Anaphase Spindle Elongation Anaphase Spindle Elongation

  year unknowncites this paper