Exploitation of Endophytic Bacteria to Enhance the Phytoremediation Potential of the Wetland Helophyte Juncus acutus

This study investigated the potential of indigenous endophytic bacteria to improve the efficiency of the wetland helophyte Juncus acutus to deal with a mixed pollution consisting of emerging organic contaminants (EOCs) and metals. The beneficial effect of bioaugmentation with selected endophytic bacteria was more prominent in case of high contamination: most of the inoculated plants (especially those inoculated with the mixed culture) removed higher percentages of organics and metals from the liquid phase in shorter times compared to the non-inoculated plants without exhibiting significant oxidative stress. When exposed to the lower concentrations, the tailored mixed culture enhanced the performance of the plants to decrease the organics and metals from the water. The composition of the root endophytic community changed in response to increased levels of contaminants while the inoculated bacteria did not modify the community structure. Our results indicate that the synergistic relationships between endophytes and the macrophyte enhance plants’ performance and may be exploited in constructed wetlands treating water with mixed contaminations. Taking into account that the concentrations of EOCs used in this study are much higher than the average contents of typical wastewaters, we can conclude that the macrophyte J. acutus with the aid of a mixed culture of tailored endophytic bacteria represents a suitable environmentally friendly alternative for treating pharmaceuticals and metals.

• Publication year

  2016

• Venue

  Frontiers in Microbiology

• Publication date

  2016-07-04

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3389/fmicb.2016.01016PMID 27458433PMCID 4930943
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• : A Review of the

  2018cited by this paper
• Bisphenol-A removal by the halophyte Juncus acutus in a phytoremediation pilot: Characterization and potential role of the endophytic community.

  2017cited by this paper
• Exposure of Cucurbita pepo to DDE-contamination alters the endophytic community: A cultivation dependent vs a cultivation independent approach.

  2016cited by this paper
• The role of halophyte Juncus acutus L. in the remediation of mixed contamination in a hydroponic greenhouse experiment

  2016influential reference
• Responses of endophytic and rhizospheric bacterial communities of salt marsh plant (Spartina alterniflora) to polycyclic aromatic hydrocarbons contamination

  2016cited by this paper
• Methods Of Enzymatic Analysis

  2016cited by this paper
• Increased growth and root Cu accumulation of Sorghum sudanense by endophytic Enterobacter sp. K3-2: Implications for Sorghum sudanense biomass production and phytostabilization.

  2016cited by this paper
• Removal of pharmaceutically active compounds (PhACs) and toxicological response of Cyperus alternifolius exposed to PhACs in microcosm constructed wetlands.

  2016cited by this paper
• A comparative assessment of intensive and extensive wastewater treatment technologies for removing emerging contaminants in small communities.

  2016cited by this paper
• Plant species affect colonization patterns and metabolic activity of associated endophytes during phytoremediation of crude oil-contaminated soil

  2016cited by this paper
• Combined endophytic inoculants enhance nickel phytoextraction from serpentine soil in the hyperaccumulator Noccaea caerulescens

  2015influential reference
• Response of two salt marsh plants to short- and long-term contamination of sediment with cadmium

  2015cited by this paper
• Enhanced remediation of sewage effluent by endophyte-assisted floating treatment wetlands

  2015cited by this paper
• The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil.

  2015influential reference
• Mitigation measures for chromium-VI contaminated groundwater - The role of endophytic bacteria in rhizofiltration.

  2015cited by this paper
• Combined amendment of immobilizers and the plant growth-promoting strain Burkholderia phytofirmans PsJN favours plant growth and reduces heavy metal uptake

  2015cited by this paper
• Potential use of Pseudomonas koreensis AGB-1 in association with Miscanthus sinensis to remediate heavy metal(loid)-contaminated mining site soil.

  2015cited by this paper
• Inoculation with endophytic Bacillus megaterium 1Y31 increases Mn accumulation and induces the growth and energy metabolism-related differentially-expressed proteome in Mn hyperaccumulator hybrid pennisetum.

  2015cited by this paper
• Improvement in phytoremediation potential of Solanum nigrum under cadmium contamination through endophytic-assisted Serratia sp. RSC-14 inoculation

  2015cited by this paper
• The use of constructed wetlands for removal of pesticides from agricultural runoff and drainage: a review.

  2015cited by this paper
• A review on the sustainability of constructed wetlands for wastewater treatment: Design and operation.

  2015influential reference
• Endophytic Cultivable Bacteria of the Metal Bioaccumulator Spartina maritima Improve Plant Growth but Not Metal Uptake in Polluted Marshes Soils

  2015cited by this paper
• Serpentine bacteria influence metal translocation and bioconcentration of Brassica juncea and Ricinus communis grown in multi-metal polluted soils

  2015cited by this paper
• ISOLATION AND CHARACTERIZATION OF FLAVONOID COMPOUND FROM FERONIA LIMONIA

  2015cited by this paper
• Exploring the rhizospheric and endophytic bacterial communities of Acer pseudoplatanus growing on a TNT-contaminated soil: towards the development of a rhizocompetent TNT-detoxifying plant growth promoting consortium

  2014cited by this paper
• Removal of pharmaceuticals and personal care products in aquatic plant-based systems: a review.

  2014cited by this paper
• Unveiling Zn hyperaccumulation in Juncus acutus: implications on the electronic energy fluxes and on oxidative stress with emphasis on non-functional Zn-chlorophylls.

  2014cited by this paper
• Enhanced degradation of textile effluent in constructed wetland system using Typha domingensis and textile effluent-degrading endophytic bacteria.

  2014cited by this paper
• How efficient are constructed wetlands in removing pharmaceuticals from untreated and treated urban wastewaters? A review.

  2014cited by this paper
• Isolation of ACC deaminase-producing habitat-adapted symbiotic bacteria associated with halophyte Limonium sinense (Girard) Kuntze and evaluating their plant growth-promoting activity under salt stress

  2014cited by this paper
• A review on removing pharmaceutical contaminants from wastewater by constructed wetlands: design, performance and mechanism.

  2014influential reference
• Zinc tolerance and accumulation in the halophytic species Juncus acutus

  2014cited by this paper
• A review on the occurrence of micropollutants in the aquatic environment and their fate and removal during wastewater treatment.

  2014influential reference
• Hydrocarbon contamination and plant species determine the phylogenetic and functional diversity of endophytic degrading bacteria

  2014cited by this paper
• R: A language and environment for statistical computing.

  2014cited by this paper
• Occurrence, transportation, monitoring and treatment of emerging micro-pollutants in waste water — A review from global views

  2013cited by this paper
• Enhancement of heavy metal phytoremediation by Alnus firma with endophytic Bacillus thuringiensis GDB-1.

  2013influential reference
• Emerging organic contaminant removal depending on primary treatment and operational strategy in horizontal subsurface flow constructed wetlands: influence of redox.

  2013cited by this paper
• Emergent plants used in free water surface constructed wetlands: A review

  2013cited by this paper
• Potential effect and accumulation of veterinary antibiotics in Phragmites australis under hydroponic conditions

  2013cited by this paper
• Performances of Two Macrophytes Species in Floating Treatment Wetlands for Cadmium, Nickel, and Zinc Removal from Urban Stormwater Runoff

  2013cited by this paper
• Construction of a plant-microbe phytoremediation system: combination of vetiver grass with a functional endophytic bacterium, Achromobacter xylosoxidans F3B, for aromatic pollutants removal.

  2013cited by this paper
• Monitoring and evaluation of antibiotic-resistant bacteria at a municipal wastewater treatment plant in China.

  2012influential reference
• Potential of willow and its genetically engineered associated bacteria to remediate mixed Cd and toluene contamination

  2012cited by this paper
• Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota

  2012cited by this paper
• Improving performance of Cytisus striatus on substrates contaminated with hexachlorocyclohexane (HCH) isomers using bacterial inoculants: developing a phytoremediation strategy

  2012cited by this paper
• Plant-microbe interactions: novel applications for exploitation in multipurpose remediation technologies.

  2012cited by this paper
• Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils.

  2011cited by this paper
• Evaluation of carbamazepine uptake and metabolization by Typha spp., a plant with potential use in phytotreatment.

  2011cited by this paper
• Endophytic bacteria improve phytoremediation of Ni and TCE co-contamination.

  2010cited by this paper
• Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, northern China.

  2010influential reference
• Quantitative Community Fingerprinting Methods for Estimating the Abundance of Operational Taxonomic Units in Natural Microbial Communities

  2009cited by this paper
• Bioaugmentation with engineered endophytic bacteria improves contaminant fate in phytoremediation.

  2009cited by this paper
• Endophytic bacteria and their potential to enhance heavy metal phytoextraction.

  2009cited by this paper
• Use of constructed wetland for the removal of heavy metals from industrial wastewater.

  2009cited by this paper
• Phytoremediation: plant-endophyte partnerships take the challenge.

  2009cited by this paper
• Antibiotics and antibiotic resistance in water environments.

  2008influential reference
• Hydrocarbon degradation potential and activity of endophytic bacteria associated with prairie plants

  2008cited by this paper
• Improving Performance?

  2008cited by this paper
• Argonaute—a database for gene regulation by mammalian microRNAs

  2005cited by this paper
• Cadmium-tolerant plant growth-promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.)

  2005cited by this paper
• Comparison of Different Primer Sets for Use in Automated Ribosomal Intergenic Spacer Analysis of Complex Bacterial Communities

  2004cited by this paper
• A Comparative Assessment

  2004cited by this paper
• Cadmium-tolerant plant growth-promoting bacteria associated with the roots of Indian mustard ( Brassica juncea L . Czern . )

  2004cited by this paper
• Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants

  2004cited by this paper
• Isolation and characterization of Populus isoperoxidases involved in the last step of lignin formation

  1985cited by this paper
• Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals

  1985cited by this paper
• Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).

  1969cited by this paper

• Endophyte synergistic phytoremediation is a sustainable solution for the removal of heavy metals and organic pollutants.

  2025cites this paper
• Functionalized FeS2@CQDs as dual-role agents: Enhancing electron availability and transport for improved nitrogen removal in constructed wetlands

  2025cites this paper
• Metataxonomic analysis of endophytic bacteria of blackberry (Rubus ulmifolius Schott) across tissues and environmental conditions

  2024cites this paper
• Enhanced removal of heavy metals by wetland plant-microbiome symbiont: Prospect of potential strategies and mechanisms for the environmental heavy metal regulation

  2024cites this paper
• Exploring macrophytes’ microbial populations dynamics to enhance bioremediation in constructed wetlands for industrial pollutants removal in sustainable wastewater treatment

  2024cites this paper
• A cyclic dipeptide for salinity stress alleviation and the trophic flexibility of endophyte provide insights into saltmarsh plant–microbe interactions

  2024cites this paper
• Plant species influences the composition of root system microbiome and its antibiotic resistance profile in a constructed wetland receiving primary treated wastewater

  2024cites this paper
• Differential interference of copper with endophytic bacterial inoculation: Atrazine decontamination in Acorus tatarinowii and culture solution.

  2023cites this paper
• A cyclic dipeptide for salinity stress alleviation and the trophic flexibility of an endophyte reveal niches in salt marsh plant-microbe interactions

  2023cites this paper
• Microbial degradation of naphthenic acids using constructed wetland treatment systems: metabolic and genomic insights for improved bioremediation of process-affected water

  2023cites this paper
• Integrated Phytobial Remediation of Dissolved Pollutants from Domestic Wastewater through Constructed Wetlands: An Interactive Macrophyte-Microbe-Based Green and Low-Cost Decontamination Technology with Prospective Resource Recovery

  2023cites this paper
• Response strategies of stem/leaves endophyte communities to nano-plastics regulate growth performance of submerged macrophytes.

  2023cites this paper
• Compost, plants and endophytes versus metal contamination: choice of a restoration strategy steers the microbiome in polymetallic mine waste

  2023cites this paper
• Current Scenario and Future Prospects of Endophytic Microbes: Promising Candidates for Abiotic and Biotic Stress Management for Agricultural and Environmental Sustainability

  2023cites this paper
• Understanding the Interaction and Potential of Halophytes and Associated Microbiome for Bio-saline Agriculture

  2023cites this paper
• Wetland Removal Mechanisms for Emerging Contaminants

  2023cites this paper
• Plant-minerals-water interactions: An investigation on Juncus acutus exposed to different Zn sources.

  2023cites this paper
• Removal of Two Triazole Fungicides from Agricultural Wastewater in Pilot-Scale Horizontal Subsurface Flow Constructed Wetlands

  2023cites this paper
• Phytoremediation as a Tool to Remove Drivers of Antimicrobial Resistance in the Aquatic Environment

  2023influential citation
• Phytoremediation as an Effective Remedy for Removing Trace Elements from Ecosystems

  2023cites this paper
• Removal of antibiotics from pharmaceutical wastewater using Lemna Aoukikusa (duckweed)

  2023cites this paper
• Differences in gene expression and endophytic bacterial diversity in Atractylodes macrocephala Koidz. rhizomes from different growth years.

  2022cites this paper
• Floating Treatment Wetlands (FTWs) is an Innovative Approach for the Remediation of Petroleum Hydrocarbons-Contaminated Water

  2022cites this paper
• Environmental Impact, Health Hazards, and Plant-Microbes Synergism in Remediation of Emerging Contaminants

  2022cites this paper
• A Review on Microorganisms in Constructed Wetlands for Typical Pollutant Removal: Species, Function, and Diversity

  2022cites this paper
• Particularities of Fungicides and Factors Affecting Their Fate and Removal Efficacy: A Review

  2022cites this paper
• Phytoaugmentation technology for phytoremediation of environmental pollutants: current scenario and future prospects

  2021cites this paper
• Genomic and Physiological Investigation of Heavy Metal Resistance from Plant Endophytic Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum

  2021cites this paper
• Diazotroph Paenibacillus triticisoli BJ-18 Drives the Variation in Bacterial, Diazotrophic and Fungal Communities in the Rhizosphere and Root/Shoot Endosphere of Maize

  2021cites this paper
• Application of floating treatment wetlands for stormwater runoff: A critical review of the recent developments with emphasis on heavy metals and nutrient removal.

  2021cites this paper
• A review on the role of plant in pharmaceuticals and personal care products (PPCPs) removal in constructed wetlands.

  2021cites this paper
• Application of Phyllosphere Microbiota as Biofertilizers

  2021cites this paper
• Recruiting endophytic bacteria of wetland plants to phytoremediate organic pollutants

  2021cites this paper
• Phylloremediation for Mitigating Air Pollution

  2021cites this paper
• Microbes-Mediated Facilitation of Micronutrients Uptake by Plants from Soil Especially Zinc

  2021cites this paper
• Heavy metal ATPase genes (HMAs) expression induced by endophytic bacteria, "AI001, and AI002" mediate cadmium translocation and phytoremediation.

  2021cites this paper
• Insights into Role of Invisible Partners in Plant Growth and Development

  2021cites this paper
• The endophytome (plant-associated microbiome): methodological approaches, biological aspects, and biotech applications

  2021cites this paper
• Phytobiomes and bioremediation

  2021cites this paper
• Fluopyram removal from agricultural equipment rinsing water using HSF pilot-scale constructed wetlands

  2021cites this paper
• Analytical Approaches for the Determination and Identification of Drug Metabolites in Plants After Uptake

  2020cites this paper
• Microbial assisted phytodepuration for water reclamation: Environmental benefits and threats.

  2020influential citation
• Enrichment of endophytic Actinobacteria in roots and rhizomes of Miscanthus × giganteus plants exposed to diclofenac and sulfamethoxazole

  2020cites this paper
• Plant Microbe Symbiosis

  2020cites this paper
• Plant-Microbiome Interactions in Hydrocarbon-Contaminated Soils

  2020cites this paper
• Metagenomic profiling of the community structure, diversity, and nutrient pathways of bacterial endophytes in maize plant

  2020cites this paper
• Diversity of bacterial endophyte in Eucalyptus clones and their implications in water stress tolerance.

  2020cites this paper
• Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Glehnia littoralis Based on Illumina Sequencing

  2020cites this paper
• Revisiting Plant–Microbe Interactions and Microbial Consortia Application for Enhancing Sustainable Agriculture: A Review

  2020cites this paper
• A review on the plant microbiome: Ecology, functions, and emerging trends in microbial application

  2019cites this paper
• Metabolization of pharmaceuticals by plants after uptake from water and soil: A review

  2019cites this paper
• The study of bacteria populations in phytoremediation of cadmium using Eichhornia crassipes

  2019cites this paper
• Plant-Endophyte Partnerships to Assist Petroleum Hydrocarbon Remediation

  2019cites this paper
• Endophytic bacteria isolated from Solanum nigrum L., alleviate cadmium (Cd) stress response by their antioxidant potentials, including SOD synthesis by sodA gene.

  2019cites this paper
• Metal Resistant Endophytic Bacteria Reduces Cadmium, Nickel Toxicity, and Enhances Expression of Metal Stress Related Genes with Improved Growth of Oryza Sativa, via Regulating Its Antioxidant Machinery and Endogenous Hormones

  2019cites this paper
• Illumina-based Analysis of Endophytic Bacterial Diversity of four Allium species

  2019cites this paper
• Metal accumulation from leachate by polyculture in crushed brick and steel slag using pilot-scale constructed wetland in the climate of Pakistan

  2019cites this paper
• Burkholderia phytofirmans PsJN and tree twigs derived biochar together retrieved Pb-induced growth, physiological and biochemical disturbances by minimizing its uptake and translocation in mung bean (Vigna radiata L.).

  2019cites this paper
• 1 Improvement of Cadmium Detoxification 2 Potential and Plant Growth Promotion by 3 Bacterial Endophytes 4

  2019cites this paper
• Floating treatment wetlands as biological buoyant filters for wastewater reclamation

  2019cites this paper
• Bacterial and fungal endophyte communities differ in trees of natural versus wastewater-treatment wetlands

  2019cites this paper
• Root Bacteria Recruited by Phragmites australis in Constructed Wetlands Have the Potential to Enhance Azo-Dye Phytodepuration

  2019influential citation
• Endophytes as Pollutant-Degrading Agents: Current Trends and Perspectives

  2019cites this paper
• Role of Rhizospheric Microbes in Soil

  2018cites this paper
• Poplar and diclofenac pollution: A focus on physiology, oxidative stress and uptake in plant organs.

  2018cites this paper
• Role of Burkholderia cepacia CS8 in Cd-stress alleviation and phytoremediation by Catharanthus roseus

  2018cites this paper
• Phytoaccumulation of Heavy Metals from Municipal Solid Waste Leachate Using Constructed Wetland

  2018cites this paper
• Removal Processes of Pharmaceuticals in Constructed Wetlands

  2018cites this paper
• Biofertilizers Based on Bacterial Endophytes Isolated from Cereals: Potential Solution to Enhance These Crops

  2018cites this paper
• Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

  2018cites this paper
• Responses of the Endophytic Bacterial Communities of Juncus acutus to Pollution With Metals, Emerging Organic Pollutants and to Bioaugmentation With Indigenous Strains

  2018cites this paper
• Microbe and plant assisted-remediation of organic xenobiotics and its enhancement by genetically modified organisms and recombinant technology: A review.

  2018cites this paper
• Juncus spp.-The helophyte for all (phyto)remediation purposes?

  2017cites this paper
• Plant beneficial rhizospheric microorganism (PBRM) strategies to improve nutrients use efficiency: A review

  2017cites this paper
• Wetlands for Wastewater Treatment

  2017cites this paper
• Rhizobial symbiosis alleviates polychlorinated biphenyls-induced systematic oxidative stress via brassinosteroids signaling in alfalfa.

  2017cites this paper
• Illumina-Based Analysis of Endophytic and Rhizosphere Bacterial Diversity of the Coastal Halophyte Messerschmidia sibirica

  2017cites this paper
• Effect of Pseudomonas fluorescens RB4 and Bacillus subtilis 189 on the phytoremediation potential of Catharanthus roseus (L.) in Cu and Pb-contaminated soils

  2017cites this paper
• Phylloremediation of Air Pollutants: Exploiting the Potential of Plant Leaves and Leaf-Associated Microbes

  2017cites this paper
• The Plant as Metaorganism and Research on Next-Generation Systemic Pesticides – Prospects and Challenges

  2016cites this paper