Engineered disulfide linking the hinge regions within lactose repressor dimer increases operator affinity, decreases sequence selectivity, and alters allostery.

No abstract is available for this paper.

• Publication year

  2001

• Venue

  Biochemistry

• Publication date

  2001-12-25

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI0114067PMID 11747440
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Strong DNA binding by covalently linked dimeric Lac headpiece: Evidence for the crucial role of the hinge helices

  2001cited by this paper
• Geometric analysis and comparison of protein-DNA interfaces: why is there no simple code for recognition?

  2000cited by this paper
• Generation of an AraC-araBAD promoter-regulated T7 expression system.

  2000cited by this paper
• Operator DNA sequence variation enhances high affinity binding by hinge helix mutants of lactose repressor protein.

  2000cited by this paper
• A closer view of the conformation of the Lac repressor bound to operator

  2000cited by this paper
• The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator.

  1999cited by this paper
• Glycine Insertion in the Hinge Region of Lactose Repressor Protein Alters DNA Binding*

  1999cited by this paper
• Hinge‐helix formation and DNA bending in various lac repressor–operator complexes

  1999cited by this paper
• DNA‐binding characteristics of the Escherichia coli CytR regulator: a relaxed spacing requirement between operator half‐sites is provided by a flexible, unstructured interdomain linker

  1998cited by this paper
• Lactose repressor protein: functional properties and structure.

  1998cited by this paper
• DNA-based Positive Control Mutants in the Binding Site Sequence of 434 Repressor*

  1998cited by this paper
• Protein‐induced fit: the CRP activator protein changes sequence‐specific DNA recognition by the CytR repressor, a highly flexible LacI member

  1997cited by this paper
• DNA-based Loss of Specificity Mutations

  1997cited by this paper
• Designed Disulfide between N-terminal Domains of Lactose Repressor Disrupts Allosteric Linkage*

  1997cited by this paper
• Protein-DNA recognition complexes: conservation of structure and binding energy in the transition state.

  1997cited by this paper
• Formation of the hinge helix in the lac represser is induced upon binding to the lac operator

  1996cited by this paper
• Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer

  1996cited by this paper
• Modified nucleotides reveal the indirect role of the central base pairs in stabilizing the lac repressor-operator complex.

  1995cited by this paper
• Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence.

  1994cited by this paper
• A thermodynamic study of the trp repressor-operator interaction.

  1994cited by this paper
• Coupling of local folding to site-specific binding of proteins to DNA.

  1994cited by this paper
• Operator sequence context influences amino acid-base-pair interactions in 434 repressor-operator complexes.

  1993cited by this paper
• A double-filter method for nitrocellulose-filter binding: application to protein-nucleic acid interactions.

  1993cited by this paper
• Lysine 84 is at the subunit interface of lac repressor protein.

  1993cited by this paper
• Non-contacted bases affect the affinity of synthetic P22 operators for P22 repressor.

  1992cited by this paper
• Definition of a consensus binding site for p53

  1992cited by this paper
• DNA twisting and the effects of non-contacted bases on affinity of 434 operator for 434 represser

  1992cited by this paper
• Deletion of lactose repressor carboxyl-terminal domain affects tetramer formation.

  1992cited by this paper
• Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons

  1991cited by this paper
• Symmetric lac operator derivatives: effects of half-operator sequence and spacing on repressor affinity.

  1990cited by this paper
• Role of the hydrophobic effect in stability of site-specific protein-DNA complexes.

  1989cited by this paper
• An NMR‐based molecular dynamics simulation of the interaction of the lac repressor headpiece and its operator in aqueous solution

  1989cited by this paper
• DNA twisting and the affinity of bacteriophage 434 operator for bacteriophage 434 repressor.

  1988cited by this paper
• Affinities of tight-binding lactose repressors for wild-type and pseudo-operators.

  1987cited by this paper
• Effect of non-contacted bases on the affinity of 434 operator for 434 repressor and Cro

  1987cited by this paper
• Cloning and characterization of several dominant-negative and tight-binding mutants of lac repressor.

  1986cited by this paper
• Thermodynamic origins of specificity in the lac repressor-operator interaction. Adaptability in the recognition of mutant operator sites.

  1985cited by this paper
• A protein structure from nuclear magnetic resonance data. lac repressor headpiece.

  1985cited by this paper
• Possible ideal lac operator: Escherichia coli lac operator-like sequences from eukaryotic genomes lack the central G X C pair.

  1984cited by this paper
• A perfectly symmetric lac operator binds the lac repressor very tightly.

  1983cited by this paper
• Characteristics of tight binding repressors of the lac operon.

  1981cited by this paper
• Sequence of the lacI gene

  1978cited by this paper
• An amino-terminal fragment of lac repressor binds specifically to lac operator.

  1978cited by this paper
• The functional repressor parts of a tetrameric lac repressor-beta-galactosidase chimaera are organized as dimers.

  1976cited by this paper
• Limited proteolytic digestion of lac repressor by trypsin. Chemical nature of the resulting trypsin-resistant core.

  1976cited by this paper
• Interaction of effecting ligands with lac repressor and repressor-operator complex.

  1975cited by this paper
• Sequences of controlling regions of the lactose operon.

  1974cited by this paper
• Lac repressor. Specific proteolytic destruction of the NH 2 -terminal region and loss of the deoxyribonucleic acid-binding activity.

  1973cited by this paper
• The amino-acid sequence of lac repressor.

  1973cited by this paper
• lac repressor--operator interaction. II. Effect of galactosides and other ligands.

  1970cited by this paper
• DNA binding of the lac repressor.

  1968cited by this paper
• The lac operator is DNA.

  1967cited by this paper
• Genetic regulatory mechanisms in the synthesis of proteins.

  1961cited by this paper

• Insights into the regulatory mechanisms and application prospects of the transcription factor Cra

  2024cites this paper
• Fructose-1-kinase has pleiotropic roles in Escherichia coli

  2023influential citation
• Potential allosteric sites captured in glycolytic enzymes via residue-based network models: Phosphofructokinase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase.

  2021cites this paper
• Transcription | lac Operon Regulation

  2021cites this paper
• Identification of Alternative Allosteric Sites in Glycolytic Enzymes for Potential Use as Species-Specific Drug Targets

  2020cites this paper
• “Multiplex” rheostat positions cluster around allosterically critical regions of the lactose repressor protein

  2020cites this paper
• Homolog comparisons further reconcile in vitro and in vivo correlations of protein activities by revealing over‐looked physiological factors

  2019cites this paper
• Reconciling in vitro and in vivo activities of engineered, LacI-based repressor proteins: Contributions of DNA looping and operator sequence variation

  2018cites this paper
• Lactose repressor hinge domain independently binds DNA

  2018cites this paper
• Protein-protein interactions with fructose-1-kinase alter function of the central Escherichia coli transcription regulator, Cra

  2017influential citation
• Revisiting Allostery In Lac Repressor

  2016cites this paper
• Data on publications, structural analyses, and queries used to build and utilize the AlloRep database

  2016cites this paper
• Insights into the Structure of Sulfolobus Nucleoid Using Engineered Sac7d Dimers with a Defined Orientation.

  2016cites this paper
• Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox Proteins*

  2015cites this paper
• Allostery and applications of the lac repressor

  2014cites this paper
• High-Resolution Specificity from DNA Sequencing Highlights Alternative Modes of Lac Repressor Binding

  2014cites this paper
• Looking for a sequence based allostery definition: a statistical journey at different resolution scales.

  2012cites this paper
• Novel insights from hybrid LacI/GalR proteins: family-wide functional attributes and biologically significant variation in transcription repression

  2012cites this paper
• Differentiating a Landscape of Lac Repressor Mediated DNA Loops Using FRET

  2011cites this paper
• Positions 94-98 of the lactose repressor N-subdomain monomer-monomer interface are critical for allosteric communication.

  2010cites this paper
• Functional impact of polar and acidic substitutions in the lactose repressor hydrophobic monomer.monomer interface with a buried lysine.

  2009cites this paper
• Allostery in the LacI/GalR family: variations on a theme.

  2009cites this paper
• Subdividing repressor function: DNA binding affinity, selectivity, and allostery can be altered by amino acid substitution of nonconserved residues in a LacI/GalR homologue.

  2008cites this paper
• Generating properly weighted ensemble of conformations of proteins from sparse or indirect distance constraints.

  2008cites this paper
• Experimental identification of specificity determinants in the domain linker of a LacI/GalR protein: Bioinformatics‐based predictions generate true positives and false negatives

  2008cites this paper
• Extrinsic interactions dominate helical propensity in coupled binding and folding of the lactose repressor protein hinge helix.

  2006cites this paper
• The lactose repressor system: paradigms for regulation, allosteric behavior and protein folding

  2006cites this paper
• Coarse-grained models of dynamic allostery in proteins.

  2005cites this paper
• EFFETS DES RADIATIONS IONISANTES SUR DES COMPLEXES ADN-PROTÉINE

  2005cites this paper
• Toward an integrated model of protein-DNA recognition as inferred from NMR studies on the Lac repressor system.

  2004cites this paper
• Structure and Flexibility Adaptation in Nonspecific and Specific Protein-DNA Complexes

  2004cites this paper
• Biological functions of the disulfides in bovine pancreatic deoxyribonuclease

  2004cites this paper
• Is allostery an intrinsic property of all dynamic proteins?

  2004cites this paper
• Thermodynamics, protein modification, and molecular dynamics in characterizing lactose repressor protein: strategies for complex analyses of protein structure-function.

  2004cites this paper
• Perturbation from a distance: mutations that alter LacI function through long-range effects.

  2003cites this paper
• Plasticity in protein–DNA recognition: lac repressor interacts with its natural operator O1 through alternative conformations of its DNA‐binding domain

  2002cites this paper
• Fine‐tuning function: Correlation of hinge domain interactions with functional distinctions between LacI and PurR

  2002cites this paper