Amygdaloid Corticotropin-releasing Factor is Involved in the Anxiolytic Effect of Acupuncture during Ethanol Withdrawal in Rats

In a previous study, acupuncture at acupoint HT7 attenuated ethanol withdrawal-induced anxiety-like behavior in rats by normalizing amygdaloid catecholamines. In the present study, the involvement of amygdaloid corticotropin-releasing factor (CRF) in the anxiolytic effect of acupuncture was investigated during ethanol withdrawal. Rats were intraperitoneally treated with 3 g /kg/day of ethanol for 28 days, and the CRF mRNA levels in the central nucleus of the amygdala (CEA) were measured by using a RT-PCR analysis 72 hours after the last dose of ethanol. During ethanol withdrawal, the rats were bilaterally treated with acupuncture at acupoints HT7, PC6 or at a non-acupoint (Tail) for one min/day for three days. Also, rats were bilaterally injected with CRF into the CEA five minutes after the third acupuncture treatment, after which followed by the elevated-plus maze (EPM) test and the plasma corticosterone radioimmunoassay (RIA) were administered. The RT-PCR analysis showed a significant increase in the amygdaloid CRF mRNA levels in the ethanol-withdrawn rats compared with both the saline-treated rats and the rats treated with acupuncture at HT7, but neither acupuncture at PC6 nor acupuncture at a non-acupoint significantly inhibited the increased mRNA expression. The EPM test and the RIA also showed that the post-acupuncture infusion of CRF greatly reduced the anxiolytic effect of acupuncture at HT7. These results suggest that during ethanol withdrawal, the anxiolytic effect of acupuncture may be mediated through the modulation of amydaloid CRF during ethanol withdrawal.

• Publication year

  2013

• Venue

  Innovations in Acupuncture and Medicine

• Publication date

  2013-02-06

• Fields of study

  Medicine

• Identifiers
  DOI 10.1016/j.jams.2013.01.017PMID 24139460
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in Rats

  2011influential reference
• Effects of acute ethanol on corticotropin-releasing hormone and β-endorphin systems at the level of the rat central amygdala

  2011cited by this paper
• NMDAR2B tyrosine phosphorylation regulates anxiety-like behavior and CRF expression in the amygdala

  2010cited by this paper
• Effects of acupuncture on chronic corticosterone-induced depression-like behavior and expression of neuropeptide Y in the rats.

  2009cited by this paper
• Brain stress systems in the amygdala and addiction.

  2009influential reference
• Intradermal acupuncture on shen-men and nei-kuan acupoints improves insomnia in stroke patients by reducing the sympathetic nervous activity: a randomized clinical trial.

  2009cited by this paper
• Noradrenergic transmission in the extended amygdala: role in increased drug-seeking and relapse during protracted drug abstinence

  2008cited by this paper
• A dose-response study of estradiol's effects on the developing zebra finch song system.

  2008cited by this paper
• [Acupuncture for the treatment of drug addiction].

  2008cited by this paper
• Differential involvement of GABA system in mediating behavioral and neurochemical effect of acupuncture in ethanol-withdrawn rats.

  2008cited by this paper
• Effect of acupuncture on anxiety-like behavior during nicotine withdrawal and relevant mechanisms.

  2008cited by this paper
• Acupuncture for the Treatment of Drug Addiction

  2008cited by this paper
• Upregulation of Ionotropic Glutamate Receptor Subunits within Specific Mesocorticolimbic Regions during Chronic Nicotine Self-Administration

  2007cited by this paper
• A Possible Mechanism Underlying the Effectiveness of Acupuncture in the Treatment of Drug Addiction

  2007cited by this paper
• Acupuncture normalizes the release of accumbal dopamine during the withdrawal period and after the ethanol challenge in chronic ethanol-treated rats.

  2006cited by this paper
• Role of the bed nucleus of the stria terminalis versus the amygdala in fear, stress, and anxiety.

  2003cited by this paper
• Abecarnil and alprazolam reverse anxiety-like behaviors induced by ethanol withdrawal.

  2000cited by this paper
• Psychological stress increased corticotropin-releasing hormone mRNA and content in the central nucleus of the amygdala but not in the hypothalamic paraventricular nucleus in the rat.

  1999cited by this paper
• The excitatory effects of the amygdala on hypothalamo-pituitary-adrenocortical responses are mediated by hypothalamic norepinephrine, serotonin, and CRF-41.

  1998cited by this paper
• Chronic infusion of a CRH1 receptor antisense oligodeoxynucleotide into the central nucleus of the amygdala reduced anxiety-related behavior in socially defeated rats.

  1995cited by this paper
• Brain corticotropin-releasing factor mediates 'anxiety-like' behavior induced by cocaine withdrawal in rats.

  1995cited by this paper
• Buspirone treatment of anxious alcoholics. A placebo-controlled trial.

  1994cited by this paper
• Corticosterone effects on corticotropin-releasing hormone mRNA in the central nucleus of the amygdala and the parvocellular region of the paraventricular nucleus of the hypothalamus.

  1994cited by this paper
• Lesions of the central nucleus of the amygdala, but not the paraventricular nucleus of the hypothalamus, block the excitatory effects of corticotropin-releasing factor on the acoustic startle reflex

  1992cited by this paper
• Rapid desensitization of the acute stimulatory effects of nicotine on rat plasma adrenocorticotropin and prolactin.

  1986cited by this paper
• The Rat Brain in Stereotaxic Coordinates

  1983influential reference
• Chinese acupuncture and moxibustion.

  1962cited by this paper
• Chinese acupuncture and moxibustion.

  1962cited by this paper

• Acupuncture for alcohol use disorder: Research status and potential strategies to improve therapeutic efficacy.

  2025cites this paper
• A protocol for the integration of multi-omics bioinformatics: Mechanism of acupuncture as an adjunctive therapy for alcohol use disorder

  2023cites this paper
• Bidirectional role of acupuncture in the treatment of drug addiction.

  2021cites this paper
• Long-term consequences of alcohol use in early adolescent mice: focus on neuroadaptations in GR, CRF and BDNF

  2021cites this paper
• Acupuncture Alleviates Anxiety and 22-kHz Ultrasonic Vocalizations in Rats Subjected to Repeated Alcohol Administration by Modulating the Brain-Derived Neurotrophic Factor/Corticotropin-Releasing Hormone Signaling Pathway

  2021cites this paper
• Role of Corticotropin-Releasing Factor in Alcohol and Nicotine Addiction.

  2020cites this paper
• Methanol extract of semen Ziziphi Spinosae attenuates ethanol withdrawal anxiety by improving neuropeptide signaling in the central amygdala

  2019cites this paper
• Driving the Downward Spiral: Alcohol-Induced Dysregulation of Extended Amygdala Circuits and Negative Affect.

  2019cites this paper
• Aqueous Extract of Semen Ziziphi Spinosae Exerts Anxiolytic Effects during Nicotine Withdrawal via Improvement of Amygdaloid CRF/CRF1R Signaling

  2018cites this paper
• Acupuncture for alcohol use disorder.

  2018influential citation
• Low doses of methylmercury intoxication solely or associated to ethanol binge drinking induce psychiatric-like disorders in adolescent female rats.

  2018cites this paper
• Acupuncture on the Stress-Related Drug Relapse to Seeking

  2018cites this paper
• Optogenetic dissection of the dopaminergic circuitry involved in memory consolidation

  2016cites this paper
• Bee Venom Acupuncture Alleviates Experimental Autoimmune Encephalomyelitis by Upregulating Regulatory T Cells and Suppressing Th1 and Th17 Responses

  2016cites this paper
• Strong Manual Acupuncture Stimulation of “Huantiao” (GB 30) Reduces Pain-Induced Anxiety and p-ERK in the Anterior Cingulate Cortex in a Rat Model of Neuropathic Pain

  2015cites this paper
• Hypothalamic Norepinephrine Mediates Acupunctural Effects on Hypothalamic—Pituitary—Adrenal Axis During Ethanol Withdrawal

  2015cites this paper
• Preclinical evidence implicating corticotropin‐releasing factor signaling in ethanol consumption and neuroadaptation

  2015cites this paper
• Korean Red Ginseng attenuates anxiety-like behavior during ethanol withdrawal in rats

  2014cites this paper
• Acupuncture Acupuncture and anxiety 2013: the year in (literature) review

  2014cites this paper
• Effects of Acupuncture at Zu-San-Li (ST36) on the Activity of the Hypothalamic—Pituitarye—drenal Axis during Ethanol Withdrawal in Rats

  2014cites this paper
• Effects of the aqueous extract of Schizandra chinensis fruit on ethanol withdrawal‐induced anxiety in rats

  2014cites this paper
• ICLE-Effects of Acupuncture at Zu-SanLi ( ST 36 ) on the Activity of the Hypothalamic e Pituitary e Adrenal Axis during Ethanol Withdrawal in Rats

  2014cites this paper
• Involvement of amygdaloid neuropeptide Y in the anxiolytic effects of acupuncture during ethanol withdrawal in rats.

  2014cites this paper