Structural basis of the specific interactions of GRAS family proteins

The plant‐specific GAI‐RGA‐and‐SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α‐helical cap and α/β core subdomains. The α‐helical cap mediates head‐to‐head heterodimerization between SHR and SCR GRAS domains. This type of dimerization is predicted for the NSP1‐NSP2 heterodimer and DELLA proteins such as RGA and SLR1 homodimers. The α/β core subdomain possesses a hydrophobic groove formed by surface α3‐ and α7‐helices and mediates protein–protein interactions. The groove of the SHR GRAS domain accommodates the zinc fingers of JKD, a BIRD/IDD family transcription factor, while the groove of the SCL7 GRAS domain mediates the SCL7 homodimerization.

• Publication year

  2018

• Venue

  FEBS Letters

• Publication date

  2018-02-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1002/1873-3468.12987PMID 29364510PMCID 5873383
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Evolutionary Analyses of GRAS Transcription Factors in Angiosperms

  2017cited by this paper
• Structure of the SHR–SCR heterodimer bound to the BIRD/IDD transcriptional factor JKD

  2017cited by this paper
• Crystal Structure of the GRAS Domain of SCARECROW-LIKE7 in Oryza sativa

  2016cited by this paper
• The Arabidopsis O-fucosyltransferase SPINDLY activates nuclear growth repressor DELLA

  2016cited by this paper
• Transcriptional control of tissue formation throughout root development

  2015cited by this paper
• A GRAS-like gene of sunflower (Helianthus annuus L.) alters the gibberellin content and axillary meristem outgrowth in transgenic Arabidopsis plants.

  2015cited by this paper
• Arabidopsis BIRD Zinc Finger Proteins Jointly Stabilize Tissue Boundaries by Confining the Cell Fate Regulator SHORT-ROOT and Contributing to Fate Specification

  2015cited by this paper
• Network of GRAS Transcription Factors Involved in the Control of Arbuscule Development in Lotus japonicus1[OPEN]

  2015cited by this paper
• DELLA protein functions as a transcriptional activator through the DNA binding of the INDETERMINATE DOMAIN family proteins

  2014cited by this paper
• DELLAs Function as Coactivators of GAI-ASSOCIATED FACTOR1 in Regulation of Gibberellin Homeostasis and Signaling in Arabidopsis[W][OPEN]

  2014cited by this paper
• Genomic analysis of DELLA protein activity.

  2013cited by this paper
• The transcription activation and homodimerization of Lotus japonicus Nod factor Signaling Pathway2 protein

  2013cited by this paper
• GRAS proteins: the versatile roles of intrinsically disordered proteins in plant signalling.

  2012cited by this paper
• Surfing along the root ground tissue gene network.

  2012cited by this paper
• Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses

  2012cited by this paper
• Brassinosteroid, gibberellin, and phytochrome impinge on a common transcription module in Arabidopsis

  2012cited by this paper
• Control of Arabidopsis root development.

  2012cited by this paper
• A GRAS-type transcription factor with a specific function in mycorrhizal signaling.

  2012cited by this paper
• Arabidopsis MYC2 Interacts with DELLA Proteins in Regulating Sesquiterpene Synthase Gene Expression[W][OA]

  2012cited by this paper
• SCARECROW-LIKE 3 promotes gibberellin signaling by antagonizing master growth repressor DELLA in Arabidopsis

  2011cited by this paper
• The molecular mechanism and evolution of the GA-GID1-DELLA signaling module in plants.

  2011cited by this paper
• DELLAs modulate jasmonate signaling via competitive binding to JAZs.

  2010cited by this paper
• Spatiotemporal regulation of cell-cycle genes by SHORTROOT links patterning and growth

  2010cited by this paper
• The salt- and drought-inducible poplar GRAS protein SCL7 confers salt and drought tolerance in Arabidopsis thaliana

  2010cited by this paper
• GRAS Proteins Form a DNA Binding Complex to Induce Gene Expression during Nodulation Signaling in Medicago truncatula[W]

  2009cited by this paper
• Gibberellin-induced DELLA recognition by the gibberellin receptor GID1

  2008cited by this paper
• An Evolutionarily Conserved Mechanism Delimiting SHR Movement Defines a Single Layer of Endodermis in Plants

  2007cited by this paper
• Arabidopsis JACKDAW and MAGPIE zinc finger proteins delimit asymmetric cell division and stabilize tissue boundaries by restricting SHORT-ROOT action.

  2007cited by this paper
• The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses.

  2007cited by this paper
• Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Arabidopsis[W]

  2007cited by this paper
• Whole-Genome Analysis of the SHORT-ROOT Developmental Pathway in Arabidopsis

  2006cited by this paper
• The role of GRAS proteins in plant signal transduction and development

  2004cited by this paper
• The Gibberellin Signaling Pathway Is Regulated by the Appearance and Disappearance of SLENDER RICE1 in Nuclei Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010319.

  2002cited by this paper
• Design and selection of novel Cys2His2 zinc finger proteins.

  2001cited by this paper
• Plant GRAS and metazoan STATs: one family?

  2000cited by this paper
• The GRAS gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW-LIKE genes.

  1999cited by this paper
• Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A

  1991cited by this paper

• Identification and expression profiling of the GRAS gene family reveals candidate genes for enhancing abiotic stress tolerance in lentil

  2026cites this paper
• Elucidating <scp> <i>R</i> </scp> ‐gene‐mediated resistance to wheat dwarf virus in <scp> <i>Triticum aestivum</i> </scp> genotypes

  2026cites this paper
• GRAS Transcription Factor WLG3 Regulates Grain Size and Weight in Rice.

  2026cites this paper
• Maize COMPACT PLANT 3 regulates plant architecture and facilitates high-density planting.

  2025cites this paper
• Overexpression of SmGRAS5 enhances tolerance to abiotic stresses in Salvia miltiorrhiza.

  2025cites this paper
• Identification and expression pattern analysis of BpGRAS gene family in Bergenia purpurascens and functional characterization of BpGRAS9 in salt tolerance

  2025cites this paper
• Structural analyses of gibberellin-mediated DELLA protein degradation

  2025cites this paper
• Genome-wide analysis of GRAS and CXE and interactions between SbDELLAs and SbGID1s in Scutellaria baicalensis Georgi

  2025cites this paper
• Genome-Wide Identification of the GRAS Transcription Factor Family in Sweet Orange and the Regulation of Salt Stress-Enhanced Plant Salt Tolerance in Sweet Orange by CsGRAS15 and CsGRAS27

  2025cites this paper
• Structural insights of gibberellin-mediated DELLA protein degradation.

  2025cites this paper
• Genome-wide identification of the GRAS gene family and evidence for the involvement of PgGRAS48 in main root development in Panax ginseng

  2025influential citation
• The GRAS protein RAM1 interacts with WRI transcription factors to regulate plant genes required for arbuscule development and function

  2025cites this paper
• SmGRAS5 Acts as a Positive Regulator in GA-induced Biosynthesis of Tanshinones in Salvia miltiorrhiza Hairy Roots.

  2025cites this paper
• The GRAS gene family and its roles in pineapple (Ananas comosus L.) developmental regulation and cold tolerance

  2024cites this paper
• Type-B response regulator RRB12 regulates nodule formation in Lotus japonicus

  2024cites this paper
• Cellular and physiological functions of SGR family in gravitropic response in higher plants

  2024cites this paper
• SlFSR positively regulates ethylene biosynthesis and lycopene accumulation during fruit ripening in tomato.

  2024cites this paper
• Enhancing arbuscular mycorrhiza symbiosis effectiveness through the involvement of the tomato GRAS transcription factor SCL3/SlGRAS18.

  2024cites this paper
• Unraveling the Molecular Mechanisms by Which the miR171b-SCL6 Module Regulates Maturation in Lilium

  2024cites this paper
• Plant tolerance to salinity stress: Regulating transcription factors and their functional role in the cellular transcriptional network

  2023cites this paper
• The GRAS protein OsDLA involves in brassinosteroid signalling and positively regulates blast resistance by forming a module with GSK2 and OsWRKY53 in rice

  2023cites this paper
• SCARECROW-like GRAS protein PES positively regulates petunia floral scent production.

  2023cites this paper
• GmBES1-1 dampens the activity of GmNSP1/2 to mediate brassinosteroid inhibition of nodulation in soybean

  2023cites this paper
• Properties of INDETERMINATE DOMAIN Proteins from Physcomitrium patens: DNA-Binding, Interaction with GRAS Proteins, and Transcriptional Activity

  2023cites this paper
• Transcriptome-Wide Identification of the GRAS Transcription Factor Family in Pinus massoniana and Its Role in Regulating Development and Stress Response

  2023cites this paper
• Transcriptional regulatory network of high-value active ingredients in medicinal plants.

  2023cites this paper
• Emerging Roles of Plant DNA-Binding With One Finger Transcription Factors in Various Hormone and Stress Signaling Pathways

  2022influential citation
• GRAS transcription factors emerging regulator in plants growth, development, and multiple stresses

  2022cites this paper
• Multifaceted roles of GRAS transcription factors in growth and stress responses in plants

  2022cites this paper
• In silico promoter analysis and expression of the BIG BROTHER gene in different organs of potato

  2022cites this paper
• Modification of tomato breeding traits and plant hormone signaling by Target-AID, the genome-editing system inducing efficient nucleotide substitution

  2022cites this paper
• Genome-Wide Identification of GRAS Transcription Factors and Their Potential Roles in Growth and Development of Rose (Rosa chinensis)

  2022influential citation
• GRAS-type transcription factor CaGRAS1 functions as a positive regulator of the drought response in Capsicum annuum

  2022cites this paper
• Genomic insights on the contribution of introgressions from Xian/Indica to the genetic improvement of Geng/Japonica rice cultivars

  2022cites this paper
• A Perspective on Developing a Plant ‘Holobiont’ for Future Saline Agriculture

  2022cites this paper
• Genome-wide survey and expression analysis of GRAS transcription factor family in sweetpotato provides insights into their potential roles in stress response

  2022cites this paper
• Autophagy-Mediated Regulation of Different Meristems in Plants

  2022cites this paper
• Genome-wide investigation of the GRAS transcription factor family in foxtail millet (Setaria italica L.)

  2021cites this paper
• SCARECROW-LIKE3 regulates the transcription of gibberellin-related genes by acting as a transcriptional co-repressor of GAI-ASSOCIATED FACTOR1

  2021cites this paper
• GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response.

  2021cites this paper
• Genome-wide Characterization of GRAS Transcription Factors and Their Potential Roles in Development and Drought Resilience in Rose (Rosa chinensis)

  2021cites this paper
• Comparative analysis of GRAS genes in six Cucurbitaceae species provides novel insights into their evolution and function

  2021cites this paper
• Genome wide identification and expression pattern analysis of the GRAS family in quinoa.

  2021cites this paper
• The Scarecrow-Like Transcription Factor SlSCL3 Regulates Volatile Terpene Biosynthesis and Glandular Trichome Size in Tomato (Solanum lycopersicum).

  2021cites this paper
• Molecular Cloning, Transcriptional Profiling, Subcellular Localization, and miRNA-Binding Site Analysis of Six SCL9 Genes in Poplar

  2021cites this paper
• Genome-wide comparison of the GRAS protein family in eight Rosaceae species and GRAS gene expression analysis in Chinese white pear (Pyrus bretschneideri Rehder)

  2021cites this paper
• Exploring the GRAS gene family in common bean (Phaseolus vulgaris L.): characterization, evolutionary relationships, and expression analyses in response to abiotic stresses

  2021cites this paper
• Early “Rootprints” of Plant Terrestrialization: Selaginella Root Development Sheds Light on Root Evolution in Vascular Plants

  2021cites this paper
• Role of gibberellin and its three GID1 receptors in Jasminum sambac stem elongation and flowering

  2021cites this paper
• Amino Acid Polymorphisms in the VHIID Conserved Motif of Nodulation Signaling Pathways 2 Distinctly Modulate Symbiotic Signaling and Nodule Morphogenesis in Medicago truncatula

  2021influential citation
• Phylogenetic analysis and target gene prediction of miR477 gene family in grape

  2021cites this paper
• Manipulating Gibberellin Control Over Growth and Fertility as a Possible Target for Managing Wild Radish Weed Populations in Cropping Systems

  2020cites this paper
• Transcriptome analysis reveals the GRAS family in Salvia miltiorrhiza hairy roots in response to gibberellin

  2020influential citation
• Transcriptomic pro les of non-embryogenic and embryogenic callus cells in a highly regenerative Upland Cotton line (Gossypium hirsutum L.)

  2020cites this paper
• Transcriptomic pro les of non-embryogenic and embryogenic callus cells in a highly regenerative Upland Cotton line (Gossypium hirsutum L.)

  2020cites this paper
• Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)

  2020cites this paper
• Transcriptomic analysis reveals the GRAS family genes respond to gibberellin in Salvia miltiorrhiza hairy roots

  2020cites this paper
• Fruit ripening in Lycium barbarum and Lycium ruthenicum is associated with distinct gene expression patterns

  2020cites this paper
• Gene Regulation via the Combination of Transcription Factors in the INDETERMINATE DOMAIN and GRAS Families

  2020cites this paper
• Genome-Wide Characterization of GRAS Family and Their Potential Roles in Cold Tolerance of Cucumber (Cucumis sativus L.)

  2020cites this paper
• Transcriptome analysis of responses in Brachypodium distachyon overexpressing the BdbZIP26 transcription factor

  2020cites this paper
• SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza

  2020cites this paper
• Identification of the GRAS gene family in the Brassica juncea genome provides insight into its role in stem swelling in stem mustard

  2019cites this paper
• Identification and evolution of GRAS transcription factor family in four Brassica species

  2019cites this paper
• SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza

  2019influential citation
• Genome-wide Identification, Classification, Expression and Duplication Analysis of GRAS Family Genes in Juglans regia L.

  2019cites this paper
• Regulation of LaSCL6 expression by genomic structure, alternative splicing, and microRNA in Larix kaempferi

  2019cites this paper
• SLR1 inhibits MOC1 degradation to coordinate tiller number and plant height in rice

  2019cites this paper
• Identification and expression analysis of StGRAS gene family in potato (Solanum tuberosum L.)

  2019cites this paper
• Genome-Wide Identification and Expression Pattern of the GRAS Gene Family in Pitaya ( Selenicereus undatus L.)

  year unknowncites this paper