Transcriptional Analysis of the 5′-Noncoding Region of the Human Involucrin Gene (*)

Human involucrin whose gene transcription is directed by a 2456-nucleotide (nt) 5′-noncoding region is a structural component of the epithelial cornified layer. Transient transfection assays demonstrated that this region is transcriptionally active in multiplying keratinocytes and is enhanced by 2 mM CaCl treatment. Calcium-independent transcriptional activity and the interaction with the AP-1 transcriptional factor was located on the proximal part (nt −159 to −1) of the 5′-noncoding region. However, CaCl responsiveness was mapped to a distal 1185-nt fragment (nt −2456 to −1272). Moreover, this fragment potentiated the Herpes simplex thymidine kinase promoter in normal keratinocytes and is responsive to calcium treatment in a cell type-specific manner. Interestingly, the absence of a 491-nt fragment located between the two enhancer domains (nt −651 to −160) resulted in transcriptional activation in multiplying keratinocytes. This fragment interacts with AP-1 and the YY1 transcriptional silencer. It is concluded that human involucrin 5′-noncoding region contains at least three regulatory domains, a distal CaCl-responsive enhancer, a putative transcriptional silencer (that interacts with AP-1 and YY1), and a proximal enhancer/promoter (that interacts with AP-1). Thus, this study demonstrates the presence of particular transcriptional factors can potentially regulate the human involucrin expression.

• Publication year

  1996

• Venue

  Journal of Biological Chemistry

• Publication date

  1996-01-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.271.1.512PMID 8550612
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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