The lactic acid bacteria metabolite phenyllactic acid inhibits both radial growth and sporulation of filamentous fungi

BackgroundFood spoilage caused by molds is a severe problem. In food and feed, e.g. dairy products, sourdough bread and silage, lactic acid bacteria are used as starter cultures. Besides lactic and acetic acid, some strains produce other low molecular weight compounds with antifungal activities. One of these metabolites is phenyllactic acid (PLA), well known for its antifungal effect. The inhibitory effect of PLA has only partially been investigated, and the objective of this study was to elucidate in detail the antifungal properties of PLA.ResultsWe investigated the outgrowth of individual conidia from Aspergillus niger, Cladosporium cladosporioides and Penicillium roqueforti, and observed the morphologies of resulting colonies on solid media using different acid concentrations. We found that PLA inhibits molds similar to weak acid preservatives. Furthermore, it has an additional activity: at sub-inhibitory concentrations, fungal colonies displayed slower radial growth and inhibited sporulation. The L isoform of PLA is a more potent inhibitor than the D form. Increased expression of phiA was observed during PLA treatment. This gene was initially identified as being induced by Streptomyces-produced macrolide antibiotics, and is shown to be a structural protein in developed cells. This suggests that PhiA may act as a general stress protectant in fungi.ConclusionFrom a food protection perspective, the results of this study support the usage of lactic acid bacteria strains synthesizing PLA as starter cultures in food and feed. Such starter cultures could inhibit spore synthesis, which would be beneficial as many food borne fungi are spread by airborne spores.

• Publication year

  2013

• Venue

  BMC Research Notes

• Publication date

  2013-11-14

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/1756-0500-6-464PMID 24229396PMCID 3835548
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2012cited by this paper
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• Trehalose 6‐phosphate phosphatase is required for cell wall integrity and fungal virulence but not trehalose biosynthesis in the human fungal pathogen Aspergillus fumigatus

  2010cited by this paper
• Auxotrophy for uridine increases the sensitivity of Aspergillus niger to weak-acid preservatives.

  2008cited by this paper
• Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

  2007cited by this paper
• Improvement of the quality and shelf life of wheat bread by fermentation with the antifungal strain Lactobacillus plantarum FST 1.7

  2007cited by this paper
• Physiological relationship between food preservatives, environmental factors, ochratoxin and otapksPV gene expression by Penicillium verrucosum.

  2007cited by this paper
• A Novel Regulator Couples Sporogenesis and Trehalose Biogenesis in Aspergillus nidulans

  2007cited by this paper
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  2007cited by this paper
• University of Groningen Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88 Pel,

  2006cited by this paper
• Antifungal lactic acid bacteria as biopreservatives

  2005cited by this paper
• Co-cultivation of antifungal Lactobacillus plantarum MiLAB 393 and Aspergillus nidulans, evaluation of effects on fungal growth and protein expression.

  2005cited by this paper
• The importance of a functional trehalose biosynthetic pathway for the life of yeasts and fungi.

  2004cited by this paper
• The Weak Acid Preservative Sorbic Acid Inhibits Conidial Germination and Mycelial Growth of Aspergillus niger through Intracellular Acidification

  2004cited by this paper
• Development of a homologous transformation system for Aspergillus niger based on the pyrG gene

  2004cited by this paper
• Lactic acid bacteria as functional starter cultures for the food fermentation industry

  2004cited by this paper
• Production of phenyllactic acid by lactic acid bacteria: an approach to the selection of strains contributing to food quality and preservation.

  2004cited by this paper
• Weak organic acid stress inhibits aromatic amino acid uptake by yeast, causing a strong influence of amino acid auxotrophies on the phenotypes of membrane transporter mutants.

  2003cited by this paper
• Antifungal Activity of Phenyllactic Acid against Molds Isolated from Bakery Products

  2003influential reference
• Characterization of phiA, a gene essential for phialide development in Aspergillus nidulans.

  2003cited by this paper
• Lactobacillus plantarum MiLAB 393 Produces the Antifungal Cyclic Dipeptides Cyclo(l-Phe-l-Pro) and Cyclo(l-Phe-trans-4-OH-l-Pro) and 3-Phenyllactic Acid

  2002cited by this paper
• Proteome analysis of Aspergillus nidulans reveals proteins associated with the response to the antibiotic concanamycin A, produced by Streptomyces species

  2002cited by this paper
• Relationship between Secondary Metabolism and Fungal Development

  2002cited by this paper
• Rhodococcus L-phenylalanine dehydrogenase: kinetics, mechanism, and structural basis for catalytic specificity.

  2000cited by this paper
• Purification and Characterization of Novel Antifungal Compounds from the Sourdough Lactobacillus plantarum Strain 21B

  2000cited by this paper
• Effect of phenylalanine and its metabolites on the proliferation and viability of neuronal and astroglial cells: possible relevance in maternal phenylketonuria.

  2000cited by this paper
• Changes in Aspergillus nidulans gene expression induced by bafilomycin, a Streptomyces-produced antibiotic.

  1999cited by this paper
• Asexual Sporulation in Aspergillus nidulans

  1998cited by this paper
• Antimicrobial spectrum and target site of D-3-phenyllactic acid.

  1998cited by this paper
• The sourdough microflora: Interactions of lactic acid bacteria and yeasts

  1998cited by this paper
• The sourdough microflora. Interactions between lactic acid bacteria and yeasts: metabolism of amino acids

  1994cited by this paper
• The sourdough microflora. Interactions between lactic acid bacteria and yeasts: metabolism of carbohydrates

  1994cited by this paper
• Antagonistic activities of lactic acid bacteria in food and feed fermentations.

  1990cited by this paper
• Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger

  1988cited by this paper
• Fungi and Food Spoilage

  1987cited by this paper
• Effect of Weak Acids on Amino Acid Transport by Penicillium chrysogenum: Evidence for a Proton or Charge Gradient as the Driving Force

  1973cited by this paper
• Effects of Acetate and Other Short-Chain Fatty Acids on Sugar and Amino Acid Uptake of Bacillus subtilis

  1972cited by this paper

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• Antifungal activity of Lactobacillus sakei PLL12 and Lactococcus lactis PLL15 against Aspergillus niger and their application in bread preservation

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  2025cites this paper
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  2025cites this paper
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• Comparative proteome analysis identifies species-specific signature proteins in Aspergillus pathogens

  2023cites this paper
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  2023cites this paper
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• Inhibition of individual and combination of cell free supernatants of phenyllactic acid, pediocin‐ and nisin‐producing lactic acid bacteria against food pathogens and bread spoilage molds

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  2022cites this paper
• Fungal solid-state fermentation of food waste for biohydrogen production by dark fermentation

  2022cites this paper
• Inhibitory effect and possible mechanism of phenyllactic acid on Aspergillus flavus spore germination

  2022cites this paper
• Energy metabolism as the target of 3-phenyllactic acid against Rhizopus oryzae.

  2022cites this paper
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  2021cites this paper
• Phenyllactic Acid: A Green Compound for Food Biopreservation

  2021cites this paper
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  2021cites this paper
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  2021cites this paper
• Antifungal Metabolites as Food Bio-Preservative: Innovation, Outlook, and Challenges

  2021cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
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