Electron Microscopic Evidence for the Transmembrane Displa cement of Calcium ATPase

Abstract Incubation of the Ca2+-ATPase in vanadate solutions leads to the formation of two-dimensional arrays in the sarcoplasmic reticulum membrane. Electron micrographic freeze fracture replicas show depressions on the inner leaflet for the first time. This indicates that the ATPase has moved perpendicular to the plane of the membrane. Our results also suggest that aggregation of the Ca2+-ATPase into the two-dimensional arrays occurs before they move into the membrane. These phenomena were observed as soon as 15 minutes after vanadate was added. The effects of vanadate appear to be completely reversible. When SR was incubated in the vanadate solutions and was then diluted into a buffer containing Ca2+ and ATP, the ATPase activity was normal for up to several hours of incubation and only somewhat reduced after 3 days.

• Publication year

  1984

• Venue

  Zeitschrift fur Naturforschung. Section C, Biosciences

• Publication date

  1984-02-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1515/znc-1984-1-230PMID 6232767
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Two-dimensional arrays of proteins in sarcoplasmic reticulum and purified Ca2+-ATPase vesicles treated with vanadate.

  1983cited by this paper
• Vanadate inhibition of the Ca2+‐dependent conformational change of the sarcoplasmic reticulum Ca2+‐ATPase

  1982cited by this paper
• The interaction of vanadate ions with the Ca-ATPase from sarcoplasmic reticulum.

  1982cited by this paper
• Regulation of the conformation transition in the Ca-ATPase from sarcoplasmic reticulum by pH, temperature, and calcium ions.

  1982cited by this paper
• Uncoupling of calcium control and phosphohydrolase activity in sarcoplasmic reticulum vesicles.

  1980cited by this paper
• Assembly of ATPase protein in sarcoplasmic reticulum membranes.

  1976cited by this paper
• Structural and chemical asymmetry of the calcium-transporting membranes of the sarcotubular system as revealed by electron microscopy.

  1967cited by this paper

• Effects of Inhibitors on Luminal Opening of Ca2+ Binding Sites in an E2P-like Complex of Sarcoplasmic Reticulum Ca22+-ATPase with Be22+-fluoride*

  2006cites this paper
• Role of water in the energy of hydrolysis of phosphate compounds--energy transduction in biological membranes.

  1989cites this paper
• Effect of urea on the partial reactions and crystallization pattern of sarcoplasmic reticulum adenosine triphosphatase.

  1988cites this paper
• Spatial organization of CaATPase molecules in sarcoplasmic reticulum vesicles.

  1987cites this paper
• Two Ca2+ ATPase genes: homologies and mechanistic implications of deduced amino acid sequences.

  1986cites this paper
• Oligovanadate binding to sarcoplasmic reticulum ATPase. Evidence for substrate analogue behavior.

  1986cites this paper
• The Density and Disposition of Ca‐ATPase in In Situ and Isolated Sarcoplasmic Reticulum a

  1986cites this paper
• Structural and mechanistic implications of the amino acid sequence of calcium-transporting ATPases.

  1986cites this paper
• Retention of ellipticity between enzymatic states of the Ca2+‐ATPase of sarcoplasmic reticulum

  1986cites this paper
• Density and disposition of Ca2+-ATPase in sarcoplasmic reticulum membrane as determined by shadowing techniques.

  1985cites this paper
• High-affinity and low-affinity vanadate binding to sarcoplasmic reticulum Ca2+-ATPase labeled with fluorescein isothiocyanate.

  1985cites this paper
• Synthesis of pyrophosphate by chromatophores of Rhodospirillum rubrum in the light and by soluble yeast inorganic pyrophosphatase in water-organic solvent mixtures.

  1985cites this paper
• Intramembranous particles are clustered on microvillus membrane vesicles.

  1985cites this paper