An assay procedure for inositol pentaphosphate in avian erythrocytes.

A method to determine the concentration of inositol pentaphosphate (IP5) in chicken erythrocytes is described. Lysates of washed erythrocytes were treated with trichloroacetic acid to remove protein and were chromatographed on Dowex-1 chloride columns. After eluting the other organic phosphates with .3 N HCl, IP5 was eluted with .5 N HCl and quantitated by phosphorus assay. The mean for 12 chickens was 15.4 +/- 2.71 micromoles/g hemoglobin. The method should be useful for the assay of large numbers of samples.

• Publication year

  1978

• Venue

  Poultry Science

• Publication date

  1978-11-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.3382/PS.0571664PMID 751041
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Differential effects of 2,3-DPG, ATP and inositol pentaphosphate (IP5) on the oxygen equilibria of duck embryonic, fetal and adult hemoglobins

  1977cited by this paper
• Studies on avian erythrocyte metabolism--I. Procedure for separation and quantitation of the major phosphorylated metabolic intermediates by anion exchange chromatography.

  1976cited by this paper
• Studies on avian erythrocyte metabolism. 5. Relationship between the major phosphorylated metabolic intermediates and while blood oxygen affinity in embryols and poults of the domestic turkey (Meleagris gallopavo).

  1976cited by this paper
• Studies on avian erythrocyte metabolism--II. Relationship between the major phosphorylated metabolic intermediates and oxygen affinity of whole blood in chick embryos and chicks.

  1976cited by this paper
• Studies on avian erythrocyte metabolism-IV. relationship between the major phosphorylated metabolic intermediates and oxygen affinity of whole blood in adults and embryos in several galliformes.

  1976cited by this paper
• Studies on avian erythrocyte metabolism. Effect of organic phosphates on oxygen affinity of embryonic and adult-type hemoglobins of the chick embryo.

  1976cited by this paper
• THE STRUCTURE OF MYO‐INOSITOL PENTAPHOSPHATES *

  1970cited by this paper
• Patterns of Phosphate Compounds in Red Blood Cells of Man and Animals

  1970cited by this paper
• Intracellular Organic Phosphates as Regulators of Oxygen Release by Haemoglobin

  1969cited by this paper
• Structure of "phytic acids"

  1969cited by this paper
• Effects of some organic phosphate compounds on the oxyhemoglobin dissociation curve in human erythrolysate.

  1968cited by this paper
• The effect of organic phosphates from the human erythrocyte on the allosteric properties of hemoglobin.

  1967cited by this paper
• Effect of organic and inorganic phosphates on the oxygen equilibrium of human erythrocytes.

  1967cited by this paper
• Studies on erythrocyte glycolysis. II. Free energy changes and rate limitings steps in erythrocyte glycolysis.

  1966cited by this paper
• Studies on erythrocyte glycolysis. I. Determination of the glycolytic intermediates in human erythrocytes.

  1965cited by this paper
• Variations in the concentration of phytic acid in the blood of the domestic fowl.

  1964cited by this paper
• Methods for the isolation of glycolytic intermediated by column chromatography with ion exchange resins.

  1959cited by this paper
• Chromatographic Separations of Inositol Phosphorus Compounds1

  1952cited by this paper
• A method for the determination of phytate phosphorus in blood.

  1941cited by this paper
• DISTRIBUTION OF ACID-SOLUBLE PHOSPHORUS IN THE BLOOD CELLS OF VARIOUS VERTEBRATES

  1941cited by this paper
• Phytic acid in avian erythro cytes.

  1940cited by this paper
• The colorimetric determination of phosphorus.

  1932cited by this paper

• The determination of myo‐inositol hexakisphosphate (phytate)

  1980cites this paper
• Changes in erythrocyte metabolism following acute blood loss in chickens.

  1978cites this paper