The impact of different mutations at Arg54 on structure, chaperone-like activity and oligomerization state of human αA-crystallin: The pathomechanism underlying congenital cataract-causing mutations R54L, R54P and R54C.

No abstract is available for this paper.

• Publication year

  2017

• Venue

  Biochimica et Biophysica Acta - Proteins and Proteomics

• Publication date

  2017-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.bbapap.2017.02.003PMID 28179137
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Human αB-crystallin as fusion protein and molecular chaperone increases the expression and folding efficiency of recombinant insulin

  2018cited by this paper
• Differential role of arginine mutations on the structure and functions of α-crystallin.

  2016cited by this paper
• Concomitant Raman spectroscopy and dynamic light scattering for characterization of therapeutic proteins at high concentrations.

  2015cited by this paper
• Real-time heterogeneous protein–protein interaction between αA-crystallin N-terminal mutants and αB-crystallin using quartz crystal microbalance (QCM)

  2015cited by this paper
• The impact of Hydrogen peroxide on structure, stability and functional properties of Human R12C mutant αA-crystallin: The imperative insights into pathomechanism of the associated congenital cataract incidence.

  2015cited by this paper
• Protein aggregation, particle formation, characterization & rheology

  2014cited by this paper
• Acetylation of Gly1 and Lys2 Promotes Aggregation of Human γD-Crystallin

  2014cited by this paper
• Evolution of crystallins for a role in the vertebrate eye lens

  2013cited by this paper
• Antiaggregation activity of chaperones and its quantification

  2013cited by this paper
• Human resistin, a proinflammatory cytokine, shows chaperone-like activity

  2013cited by this paper
• A R54L Mutation of CRYAA Associated with Autosomal Dominant Nuclear Cataracts in a Chinese Family

  2013cited by this paper
• Effect of homocysteinylation on structure, chaperone activity and fibrillation propensity of lens alpha-crystallin.

  2013cited by this paper
• Interaction of thioflavin T with amyloid fibrils: fluorescence quantum yield of bound dye.

  2012cited by this paper
• A novel mutation in CRYAA is associated with autosomal dominant suture cataracts in a Chinese family

  2012cited by this paper
• Cataract-Causing Defect of a Mutant γ-Crystallin Proceeds through an Aggregation Pathway Which Bypasses Recognition by the α-Crystallin Chaperone

  2012cited by this paper
• How to Quantify the Chaperone-Like (Anti-Aggregation) Activity?

  2012cited by this paper
• Acetylation of αA-crystallin in the human lens: effects on structure and chaperone function.

  2012cited by this paper
• Quaternary structural parameters of the congenital cataract causing mutants of αA-crystallin

  2011cited by this paper
• The ageing lens and cataract: a model of normal and pathological ageing

  2011cited by this paper
• Cataract-associated mutant E107A of human γD-crystallin shows increased attraction to α-crystallin and enhanced light scattering

  2010cited by this paper
• Cat-Map: putting cataract on the map

  2010cited by this paper
• Chaperone‐like activities of different molecular forms of β‐casein. Importance of polarity of N‐terminal hydrophilic domain

  2009cited by this paper
• A novel mutation in CRYBB2 responsible for inherited coronary cataract

  2009cited by this paper
• Genetics of crystallins: cataract and beyond.

  2009cited by this paper
• Selective Cu2+ binding, redox silencing, and cytoprotective effects of the small heat shock proteins alphaA- and alphaB-crystallin.

  2008cited by this paper
• Alpha-crystallin.

  2008cited by this paper
• Crystallin gene mutations in Indian families with inherited pediatric cataract

  2008cited by this paper
• Age-dependent association of γ-crystallins with aged α-crystallins from old bovine lens

  2008cited by this paper
• Susceptibility of ovine lens crystallins to proteolytic cleavage during formation of hereditary cataract.

  2008cited by this paper
• Immobilized metal affinity electrophoresis: a novel method of capturing phosphoproteins by electrophoresis.

  2008cited by this paper
• ANS fluorescence: potential to augment the identification of the external binding sites of proteins.

  2007cited by this paper
• α-Crystallin Assisted Refolding of Enzyme Substrates: Optimization of External Parameters

  2007cited by this paper
• Screening of crystallin-crystallin interactions using microequilibrium dialysis.

  2005cited by this paper
• How to study proteins by circular dichroism.

  2005cited by this paper
• Age-related nuclear cataract-oxidation is the key.

  2005cited by this paper
• Differential scanning calorimetry in life science: thermodynamics, stability, molecular recognition and application in drug design.

  2005cited by this paper
• Heat capacity in proteins.

  2005cited by this paper
• Amyloid Fibril Formation by Lens Crystallin Proteins and Its Implications for Cataract Formation*

  2004cited by this paper
• DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data

  2004cited by this paper
• Equilibrium thermal transitions of collagen model peptides

  2004cited by this paper
• Human alphaA- and alphaB-crystallins bind to Bax and Bcl-X(S) to sequester their translocation during staurosporine-induced apoptosis.

  2004cited by this paper
• Human αA- and αB-crystallins bind to Bax and Bcl-XS to sequester their translocation during staurosporine-induced apoptosis

  2004cited by this paper
• Ageing and vision: structure, stability and function of lens crystallins.

  2004cited by this paper
• Alteration of protein-protein interactions of congenital cataract crystallin mutants.

  2003cited by this paper
• Kinetics of Protein Aggregation. Quantitative Estimation of the Chaperone-Like Activity in Test-Systems Based on Suppression of Protein Aggregation

  2002cited by this paper
• Temperature-dependent Chaperone Activity and Structural Properties of Human αA- and αB-crystallins*

  2000cited by this paper
• Temperature-dependent chaperone activity and structural properties of human alphaA- and alphaB-crystallins.

  2000cited by this paper
• Age-related changes in human lens crystallins identified by HPLC and mass spectrometry.

  1998cited by this paper
• Kinetics of heat aggregation of proteins.

  1998cited by this paper
• Protection of enzymes by alpha-crystallin acting as a molecular chaperone.

  1998cited by this paper
• Aggregation in aqueous solutions of bovine lens gamma-crystallins: special role of gamma(s).

  1998cited by this paper
• Protection of enzymes by α-crystallin acting as a molecular chaperone

  1998cited by this paper
• Identification of 1,1′-Bi(4-anilino)naphthalene-5,5′-disulfonic Acid Binding Sequences in α-Crystallin*

  1998cited by this paper
• On the substrate specificity of alpha-crystallin as a molecular chaperone.

  1995cited by this paper
• How to measure and predict the molar absorption coefficient of a protein

  1995cited by this paper
• Alpha-crystallin/small heat shock protein has autokinase activity.

  1994cited by this paper
• Suppression of phase separation in bovine gamma IV crystallin solutions: effect of modification by charged versus uncharged polar groups.

  1993cited by this paper
• Small heat shock proteins are molecular chaperones.

  1993cited by this paper
• alpha A-crystallin is expressed in non-ocular tissues.

  1992cited by this paper
• alpha B subunit of lens-specific protein alpha-crystallin is present in other ocular and non-ocular tissues.

  1989cited by this paper
• αB subunit of lens-specific protein α-crystallin is present in other ocular and non-ocular tissues

  1989cited by this paper
• Domain structure and evolution in α‐crystallins and small heat‐shock proteins

  1985cited by this paper
• Domain structure and evolution in alpha-crystallins and small heat-shock proteins.

  1985cited by this paper
• Maintenance of optical quality during crystalline lens growth

  1983cited by this paper
• Short-range order of crystallin proteins accounts for eye lens transparency

  1983cited by this paper
• The Amino-Acid Sequence of the αA2 Chain of Bovine α-Crystallin

  1973cited by this paper
• The amino-acid sequence of the alphaA2 chain of bovine alpha-crystallin.

  1973cited by this paper
• The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

  1969cited by this paper

• Recent Insights into the Pathomechanisms of Cataract Development in Diabetic Patients.

  2025cites this paper
• Structural and functional impact of the p.R163C mutation in the conserved palindromic motif within the C-terminal domain of human αB-crystallin

  2025cites this paper
• Structural and functional consequences of the cardiomyopathy‐associated p.R157C mutation in the C‐terminal palindromic motif of human αB‐crystallin

  2025cites this paper
• Small HSPs at the crossroad between protein aggregation, autophagy and unconventional secretion: clinical implications and potential therapeutic opportunities in the context of neurodegenerative diseases

  2025cites this paper
• The p.K90N Mutation in Human HSPB5 Highlights the Critical Role of Lysine 90 in Chaperone Function and Structural Integrity.

  2025cites this paper
• Structural characterization and functional analysis of human αB-crystallin with the p.R11G mutation: Insights into cataractogenesis and cardiomyopathy.

  2025cites this paper
• Role of Tau and Amyloid-beta in autophagy gene dysregulation through oxidative stress.

  2025cites this paper
• Histidine Tags in Human Recombinant Alpha B‐Crystallin (HSPB5) Proteins Are Detrimental for Zinc Binding Studies

  2025cites this paper
• The p.P51L mutation in human HspB5: Structural and functional changes linked to cardiomyopathy and cataract pathogenesis.

  2025cites this paper
• The role of the N-terminal p.R56Q mutation in modulating oligomerization and chaperone activity of human αB-crystallin in relation to cardiomyopathy.

  2025cites this paper
• Understanding the structural and functional changes and biochemical pathomechanism of the cardiomyopathy-associated p.R123W mutation in human αB-crystallin.

  2024cites this paper
• Influence of Cataract Causing Mutations on αA-Crystallin: A Computational Approach

  2024cites this paper
• Insights into the dual nature of αB-crystallin chaperone activity from the p.P39L mutant at the N-terminal region

  2024influential citation
• Exploring the significance of potassium homeostasis in copper ion binding to human αB-Crystallin.

  2024cites this paper
• Efficient Expression in the Prokaryotic Host System, Purification and Structural Analyses of the Recombinant Human ACE2 Catalytic Subunit as a Hybrid Protein with the B Subunit of Cholera Toxin (CTB-ACE2)

  2023cites this paper
• HspB5 Chaperone Structure and Activity Are Modulated by Chemical-Scale Interactions in the ACD Dimer Interface

  2023cites this paper
• Investigating the role of double mutations R12C/P20R, and R12C/R69C on structure, chaperone-like activity, and amyloidogenic properties of human αB-crystallin.

  2023cites this paper
• The structural and functional consequences of melatonin and serotonin on human αB-crystallin and their dual role in the eye lens transparency.

  2023cites this paper
• Increased chaperone activity of human α‌B-crystallin with incomplete oxidation as a new defense mechanism against oxidative stress.

  2022cites this paper
• Relationship between the Structure and Chaperone Activity of Human αA-Crystallin after Its Modification with Diabetes-Associated Oxidative Agents and Protective Role of Antioxidant Compounds

  2022cites this paper
• Связь между структурой и шаперонной активностью αA-кристаллина человека после его модификации ассоциированными с сахарным диабетом окислительными агентами и защитная роль антиоксидантных молекул

  2022cites this paper
• Insights on Human Small Heat Shock Proteins and Their Alterations in Diseases

  2022cites this paper
• Protein Aggregation and Cataract: Role of Age-Related Modifications and Mutations in α-Crystallins

  2022cites this paper
• The Role of Small Heat Shock Proteins in Protein Misfolding Associated Motoneuron Diseases

  2022cites this paper
• The biochemical association between R157H mutation in human αB-crystallin and development of cardiomyopathy: Structural and functional analyses of the mutant protein.

  2021cites this paper
• Structural and functional studies of D109A human αB-crystallin contributing to the development of cataract and cardiomyopathy diseases

  2021cites this paper
• Analysis of the data on titration of native and peroxynitrite modified αA- and αB-crystallins by Cu2+-ions

  2020cites this paper
• The congenital cataract-causing mutations P20R and A171T are associated with important changes in the amyloidogenic feature, structure and chaperone-like activity of human αB-crystallin.

  2020cites this paper
• Nucleosomal association and altered interactome underlie the mechanism of cataract caused by the R54C mutation of αA-crystallin

  2020cites this paper
• A new heterozygous mutation in the stop codon of CRYAB (p.X176Y) is liable for congenital posterior pole cataract in a Chinese family.

  2020cites this paper
• Structural and functional characterization of D109H and R69C mutant versions of human αB-crystallin: the biochemical pathomechanism underlying cataract and myopathy development.

  2020cites this paper
• Kinetic data analysis of chaperone-like activity of Wt, R69C and D109H αB-crystallins

  2019cites this paper
• Molecular genetics of congenital cataracts.

  2019cites this paper
• Asp 58 modulates lens αA‐crystallin oligomer formation and chaperone function

  2018cites this paper
• Importance of the positively charged residue at position 54 to the chaperoning function, conformational stability and amyloidogenic nature of human &agr;A-crystallin

  2018cites this paper
• Human αB-crystallin as fusion protein and molecular chaperone increases the expression and folding efficiency of recombinant insulin

  2018cites this paper
• Applications of Immobilized Metal Affinity Electrophoresis.

  2018cites this paper
• Structural and functional alteration of human αA-crystallin after exposure to full spectrum solar radiation and preventive role of lens antioxidants.

  2018cites this paper
• The cataract-causing mutation G75V promotes γS-crystallin aggregation by modifying and destabilizing the native structure.

  2018cites this paper
• Protective role of antioxidant compounds against peroxynitrite-mediated modification of R54C mutant αA-crystallin.

  2017cites this paper
• The Double-Edged Sword of α B-Crystallin Chaperone Activity: Insights from P39L Mutant at N-terminal region

  year unknowncites this paper