Purification and Properties of Chalcone Synthase from Cell Suspension Cultures of Soybean

Incubation of soybean cell suspension cultures in B-5 medium containing 0.4 ᴍ sucrose caused induction of chalcone synthase (CHS) up to a specific activity of 8 µkat/kg. CHS from these cultures was purified to apparent homogeneity by a 5-step procedure. Isoelectric focussing of pure CHS gave a major band at pH 5.45 and two weaker bands at pH 5.35 and 5.5. At least the bands at pH 5.35 and 5.45 had CHS activity. Analysis of pure CHS by two-dimensional electrophoresis gave a set ot six proteins with a M, of 40 kDa and pIs between 6.0 and 6.6. One-dimensional PAGE of CHS under non-denaturing conditions gave three closely spaced protein bands. A specific antibody was raised against soybean CHS which cross-reacted with parsley CHS. Attempts to find synthesis of deoxychalconc or of the corresponding 7.4′-dihydroxyflavanonc with CHS of different purification stages and with various cofactors failed.

• Publication year

  1987

• Venue

  Zeitschrift für Naturforschung C

• Publication date

  1987-12-01

• Fields of study

  Agricultural and Food Sciences, Biology

• Identifiers
  DOI 10.1515/znc-1987-11-1211
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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