Activation of apoptotic protein in U937 cells by a component of turmeric oil.

Aromatic (ar)-turmerone from turmeric oil displays anti-tumorigenesis activity that includes inhibited cell proliferation. This study investigated ar-turmerone-mediated apoptotic protein activation in human lymphoma U937 cells. Ar-turmerone treatment inhibited U937 cell viability in a concentration-dependent fashion, with inhibition exceeding 84%. Moreover, the treatment produced nucleosomal DNA fragmentation and the percentage of sub-diploid cells increased in a concentration-dependent manner; both are hallmarks of apoptosis. The apoptotic effect of ar-turmerone was associated with the induction of Bax and p53 proteins, rather than Bcl-2 and p21. Activation of mitochondrial cytochrome c and caspase-3 demonstrated that the activation of caspases accompanied the apoptotic effect of ar-turmerone, which mediated cell death. These results suggest that the apoptotic effect of ar-turmerone on U937 cells may involve caspase-3 activation through the induction of Bax and p53, rather than Bcl-2 and p21.

• Publication year

  2009

• Venue

  BMB Reports

• Publication date

  2009-02-28

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.5483/BMBREP.2009.42.2.096PMID 19250610
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The cytotoxic and anti-proliferative effects of 3-hydrogenkwadaphnin in K562 and jurkat cells is reduced by guanosine.

  2005cited by this paper
• Induction of apoptosis by ar-turmerone on various cell lines.

  2004cited by this paper
• Extraction of essential oil and pigments from Curcuma longa [L] by steam distillation and extraction with volatile solvents.

  2003cited by this paper
• Sesquiterpenoids from the rhizome of Curcuma zedoaria.

  2001cited by this paper
• Mammalian caspases: structure, activation, substrates, and functions during apoptosis.

  1999cited by this paper
• Bax-induced Caspase Activation and Apoptosis via Cytochromec Release from Mitochondria Is Inhibitable by Bcl-xL*

  1999influential reference
• Mitochondria in chemotherapy-induced apoptosis: a prospective novel target of cancer therapy (review).

  1998cited by this paper
• BCL-2 family: regulators of cell death.

  1998cited by this paper
• Properties of the caspases.

  1998cited by this paper
• Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c

  1998cited by this paper
• The mitochondrial death/life regulator in apoptosis and necrosis.

  1998cited by this paper
• Novel bioactivities of Curcuma longa constituents.

  1998cited by this paper
• Role of Bcl‐2 family proteins in apoptosis: apoptosomes or mitochondria?

  1998cited by this paper
• Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.

  1997cited by this paper
• Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis.

  1997cited by this paper
• Estrogen reduces proliferation and agonist-induced calcium increase in coronary artery smooth muscle cells.

  1997cited by this paper
• The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis

  1997cited by this paper
• Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c.

  1996cited by this paper
• Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo.

  1994cited by this paper
• The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme.

  1993cited by this paper
• The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1β-converting enzyme

  1993cited by this paper
• p53-dependent apoptosis modulates the cytotoxicity of anticancer agents.

  1993cited by this paper
• WAF1, a potential mediator of p53 tumor suppression.

  1993cited by this paper
• A novel heterodimeric cysteine protease is required for interleukin-1βprocessing in monocytes

  1992cited by this paper
• bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocytes.

  1991cited by this paper
• An in vitro chemosensitivity test for the screening of anti‐cancer drugs in childhood leukemia

  1990cited by this paper
• Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.

  1983cited by this paper
• Cell death: the significance of apoptosis.

  1980cited by this paper

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  2026cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2016cites this paper
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  2015cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2013cites this paper
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  2012cites this paper
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  2012cites this paper
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  2011cites this paper
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  2011cites this paper
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  2010cites this paper
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