Optimal detection angle in sub-diffraction resolution photothermal microscopy: application for high sensitivity imaging of biological tissues.

We evaluated the optimal detection angle for maximizing the signal to noise ratio (SNR) in sub-diffraction resolution photothermal microscopy. The angular dependent photothermal signal was calculated based on scattering theory using the temporally modulated Yukawa potential, and its detection angle and modulation frequency dependencies were analyzed. We verified the theoretical findings by imaging gold nanoparticles using laser diode based photothermal microscopy with balanced detection scheme. High-sensitivity (SNR ~40) photothermal biological imaging of a mouse brain was also demonstrated.

• Publication year

  2014

• Venue

  Optics Express

• Publication date

  2014-08-11

• Fields of study

  Materials Science, Medicine, Physics, Engineering

• Identifiers
  DOI 10.1364/OE.22.018833PMID 25320969
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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