Sexing chick mRNA: A protocol based on quantitative real‐time polymerase chain reaction

&NA; The accurate identification of sex in birds is important for research on avian sex determination and differentiation. Polymerase chain reaction (PCR)‐based methods have been widely applied for the molecular sexing of birds. However, these methods have used genomic DNA. Here, we present the first sexing protocol for chick mRNA based on real‐time quantitative PCR. We demonstrate that this method can accurately determine sex using mRNA from chick gonads and other tissues, such as heart, liver, spleen, lung, and muscle. The strategy of this protocol also may be suitable for other species in which sex is determined by the inheritance of sex chromosomes (ZZ male and ZW female).

• Publication year

  2016

• Venue

  Poultry Science

• Publication date

  2016-09-24

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3382/ps/pew338PMID 27665010
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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