Purification of common light chain IgG-like bispecific antibodies using highly linear pH gradients

No abstract is available for this paper.

• Publication year

  2017

• Venue

  mAbs

• Publication date

  2017-01-11

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1080/19420862.2016.1267090PMID 27937066PMCID PMC5297495
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Scale-Up of Protein Purification: Downstream Processing Issues.

  2017cited by this paper
• Continuous counter‐current chromatography for capture and polishing steps in biopharmaceutical production

  2016cited by this paper
• Application of linear pH gradients for the modeling of ion exchange chromatography: Separation of monoclonal antibody monomer from aggregates.

  2016cited by this paper
• Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG

  2015cited by this paper
• Alternative molecular formats and therapeutic applications for bispecific antibodies.

  2015cited by this paper
• Solvent modulated linear pH gradient elution for the purification of conventional and bispecific antibodies: Modeling and application.

  2015cited by this paper
• Improving target cell specificity using a novel monovalent bispecific IgG design

  2015cited by this paper
• Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface

  2014cited by this paper
• Modeling of salt and pH gradient elution in ion-exchange chromatography.

  2014cited by this paper
• Identification and Multidimensional Optimization of an Asymmetric Bispecific IgG Antibody Mimicking the Function of Factor VIII Cofactor Activity

  2013influential reference
• Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange

  2013cited by this paper
• Improving biophysical properties of a bispecific antibody scaffold to aid developability

  2013cited by this paper
• Addressing polyspecificity of antibodies selected from an in vitro yeast presentation system: a FACS-based, high-throughput selection and analytical tool.

  2013cited by this paper
• Systematic generation of buffer systems for pH gradient ion exchange chromatography and their application.

  2013cited by this paper
• Improving pH gradient cation-exchange chromatography of monoclonal antibodies by controlling ionic strength.

  2013cited by this paper
• Generating bispecific human IgG1 and IgG2 antibodies from any antibody pair.

  2012cited by this paper
• Anti-EGFR biparatopic-SEED antibody has enhanced combination-activity in a single molecule.

  2012cited by this paper
• Antitumor Activity of a Novel Bispecific Antibody That Targets the ErbB2/ErbB3 Oncogenic Unit and Inhibits Heregulin-Induced Activation of ErbB3

  2012cited by this paper
• State‐of‐the‐art in downstream processing of monoclonal antibodies: Process trends in design and validation

  2012cited by this paper
• Anti‐CD3 × anti‐GD2 bispecific antibody redirects T‐cell cytolytic activity to neuroblastoma targets

  2012cited by this paper
• LUZ-Y, a Novel Platform for the Mammalian Cell Production of Full-length IgG-bispecific Antibodies*

  2012cited by this paper
• Dual targeting strategies with bispecific antibodies

  2012cited by this paper
• A dual‐targeting antibody against EGFR‐VEGF for lung and head and neck cancer treatment

  2012cited by this paper
• Validation of a pH gradient-based ion-exchange chromatography method for high-resolution monoclonal antibody charge variant separations.

  2011cited by this paper
• Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies

  2011cited by this paper
• A two-in-one antibody against HER3 and EGFR has superior inhibitory activity compared with monospecific antibodies.

  2011cited by this paper
• Enhancing Antibody Fc Heterodimer Formation through Electrostatic Steering Effects

  2010cited by this paper
• Chemical generation of bispecific antibodies

  2010cited by this paper
• Effector cell recruitment with novel Fv-based dual-affinity re-targeting protein leads to potent tumor cytolysis and in vivo B-cell depletion.

  2010cited by this paper
• Anti-tumor activity of stability-engineered IgG-like bispecific antibodies targeting TRAIL-R2 and LTβR

  2009cited by this paper
• Bispecific T-cell engaging antibodies for cancer therapy.

  2009cited by this paper
• Variants of the Antibody Herceptin That Interact with HER2 and VEGF at the Antigen Binding Site

  2009cited by this paper
• Multiproduct high-resolution monoclonal antibody charge variant separations by pH gradient ion-exchange chromatography.

  2009cited by this paper
• Engineering of stable bispecific antibodies targeting IL-17A and IL-23.

  2009cited by this paper
• Anti-tumor activity of stability-engineered IgG-like bispecific antibodies targeting TRAIL-R2 and LTbetaR.

  2009cited by this paper
• Theory and applications of a novel ion exchange chromatographic technology using controlled pH gradients for separating proteins on anionic and cationic stationary phases.

  2008cited by this paper
• Selection of pH-related parameters in ion-exchange chromatography using pH-gradient operations.

  2008cited by this paper
• Simultaneous targeting of multiple disease mediators by a dual-variable-domain immunoglobulin

  2007cited by this paper
• Bispecific human IgG by design.

  2001cited by this paper
• Diversity in the CDR3 region of V(H) is sufficient for most antibody specificities.

  2000influential reference
• EMBOSS: the European Molecular Biology Open Software Suite.

  2000cited by this paper
• An efficient route to human bispecific IgG

  1998cited by this paper

• A single column multimodal cation exchange process for removal of half antibody, homodimer and light chain mispaired product-related impurities from a bispecific antibody.

  2026cites this paper
• Trends and challenges in bispecific antibody production.

  2025cites this paper
• Development of a bispecific antibody-homodimer separation process by cation exchange chromatography based on mechanistic modelling.

  2025cites this paper
• Design of orthogonal constant domain interfaces to aid proper heavy/light chain pairing of bispecific antibodies

  2025cites this paper
• Anti-HER2 biparatopic antibody KJ015 has near-native structure, functional balanced high affinity, and synergistic efficacy with anti-PD-1 treatment in vivo

  2024cites this paper
• Developability considerations for bispecific and multispecific antibodies

  2024cites this paper
• Optimization of the polishing process by integrating experimental design and high-throughput screening

  2024cites this paper
• Structural study of a light chain mispaired bispecific predicts mechanism of downstream separation.

  2024cites this paper
• Effect of glass fibre on the strength and durability of artificially cemented alluvial clay

  2024cites this paper
• DEVELOPMENT OF A 10G/L PROCESS FOR A DIFFICULT-TO-EXPRESS MULTISPECIFIC ANTIBODY FORMAT USING A HOLISTIC PROCESS DEVELOPMENT APPROACH.

  2024cites this paper
• Purification of anti spike SARS-CoV-2 monoclonal antibodies using ion exchange chromatography with different pH conditions

  2024cites this paper
• Facilitating high throughput bispecific antibody production and potential applications within biopharmaceutical discovery workflows

  2024cites this paper
• Bispecific Antibodies, Immune Checkpoint Inhibitors, and Antibody−Drug Conjugates Directing Antitumor Immune Responses: Challenges and Prospects

  2024cites this paper
• The downstream purification of bispecific antibodies.

  2024cites this paper
• Separation of bispecific antibody related impurities with mixed-mode chromatography

  2023cites this paper
• Structure-based engineering of a novel CD3ε-targeting antibody for reduced polyreactivity

  2023cites this paper
• Effectively removing the homodimer in bispecific antibodies by weak partitioning mode of anion exchange chromatography.

  2023cites this paper
• Tuning the Assembly of Bispecific Antibodies by Playing on Differential Polypeptide Chain Molar Ratios

  2023cites this paper
• Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification

  2023cites this paper
• Two-peak Elution Profile of a Bispecific VHH-IgG Fusion Protein in Ion Exchange Purification Process caused by Atypical N-Glycosylation

  2022cites this paper
• Ceramic hydroxyapatite chromatography plays a critical role in bispecific antibody purification process for impurity removal.

  2022cites this paper
• Effective flow-through polishing strategies for knob-into-hole bispecific antibodies

  2022cites this paper
• The manufacturing considerations of bispecific antibodies

  2022cites this paper
• Principles and Current Clinical Landscape of Multispecific Antibodies against Cancer

  2021cites this paper
• Systematic workflow for studying domain contributions of bispecific antibodies to selectivity in multimodal chromatography

  2021cites this paper
• Current trends and challenges in the downstream purification of bispecific antibodies

  2021cites this paper
• A roadmap for IgG-like bispecific antibody purification

  2020cites this paper
• Engineered Technologies and Bioanalysis of multispecific Antibody Formats

  2020cites this paper
• A robust heterodimeric Fc platform engineered for efficient development of bispecific antibodies of multiple formats.

  2019cites this paper
• A brief introduction of IgG-like bispecific antibody purification: Methods for removing product-related impurities.

  2019cites this paper
• Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies

  2018cites this paper
• Emerging trends in bispecific antibody and scaffold protein therapeutics

  2018cites this paper
• Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies

  2017cites this paper