Performance of light-emitting diode-based fluorescence microscopy to diagnose tuberculosis in a rural hospital of ethiopia

In resource-limited settings such as Gambo General Hospital (Oromya Region, Ethiopia) that see a high incidence of tuberculosis (TB), diagnosis of pulmonary TB (PTB) is mainly made by sputum examination using microscopy for acid-fast bacilli (AFB).[1,2] However, the sensitivity of the test is poor, and case detection rates are low. In rural areas of low-income countries, mycobacterial culture or automated methods as GeneXpert MTB/RIF assay are generally not available for the rapid diagnosis of TB.[3] Fluorescence microscopy (FM) using auramine O staining is more sensitive than conventional microscopy using Ziehl–Neelsen (ZN) staining.[1-3] However, the infrastructure necessary for conventional FM is not possible in most health services due to high cost; short life of the specialized mercury lamp; higher lamp warm-up time, maintenance, and alignment; and need for dark examination rooms.[3,4] New FMs employing light-emitting diodes (LED-FM) have the combined advantages of light and FM while minimizing their disadvantages; they are inexpensive, easy to maintain and operate and do not require a dark room.[3,4]

• Publication year

  2017

• Venue

  International Journal of Mycobacteriology

• Publication date

  2017-04-01

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.4103/ijmy.ijmy_43_17PMID 28559530
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Diagnostic accuracy of same-day microscopy versus standard microscopy for pulmonary tuberculosis: a systematic review and meta-analysis.

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• Performance of light-emitting diode fluorescence microscope for diagnosis of tuberculosis.

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