Hypocholesterolemic Effects of Probiotic Mixture on Diet-Induced Hypercholesterolemic Rats

Growing evidence has indicated that supplementation with probiotics improves lipid metabolism. We aimed to investigate the beneficial effects of a probiotics mixture (PM) of three strains belonging to the species Bifidobacterium (B. longum, B. lactis, and B. breve) and two strains belonging to the species Lactobacillus (L. reuteri and L. plantarum) on cholesterol-lowering efficacy in hypercholesterolemic rats. A hypercholesterolemic rat model was established by feeding a high-cholesterol diet for eight weeks. To test the effects of PM on hypercholesterolemia, hypercholesterolemic rats were assigned to four groups, which were treated daily with low (1.65 × 109 cfu/kg), medium (5.5 × 109 cfu/kg), or high (1.65 × 1010 cfu/kg) doses of probiotic mixture or simvastatin for eight weeks. Significant reductions of serum total cholesterol (TC), triacylglycerol (TG), and low-density lipoprotein (LDL)-cholesterol levels, but increases of high-density lipoprotein (HDL)-cholesterol were observed after supplementation of PM in hypercholesterolemic rats. In PM-supplemented hypercholesterolemic rats, hepatic tissue contents of TC and TG also significantly decreased. Notably, the histological evaluation of liver tissues demonstrated that PM dramatically decreased lipid accumulation. For their underlying mechanisms, we demonstrated that PM reduced expressions of cholesterol synthesis-related proteins such as sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) in the liver. Taken together, these findings suggest that PM has beneficial effects against hypercholesterolemia. Accordingly, our PM might be utilized as a novel therapeutic agent for the management of hypercholesterolemia.

• Publication year

  2017

• Venue

  Nutrients

• Publication date

  2017-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3390/nu9030293PMID 28300786PMCID 5372956
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Probiotic mixture improves fatty liver disease by virtue of its action on lipid profiles, leptin, and inflammatory biomarkers

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  2017cited by this paper
• The Year in Cardiology 2013: cardiovascular disease prevention.

  2014cited by this paper
• The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model

  2014cited by this paper
• Effect of pigeon pea (Cajanus cajan L.) on high-fat diet-induced hypercholesterolemia in hamsters.

  2013cited by this paper
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  2013cited by this paper
• Hypocholesterolemic Action of Lactobacillus plantarum NRRL-B-4524 and Lactobacillus paracasei in Mice with Hypercholesterolemia Induced by Diet .

  2012cited by this paper
• Nonalcoholic fatty liver disease (NAFLD) activity score and the histopathologic diagnosis in NAFLD: distinct clinicopathologic meanings

  2011cited by this paper
• Angiotensin I-converting enzyme inhibitory activity and bioconversion of isoflavones by probiotics in soymilk supplemented with prebiotics

  2010cited by this paper
• The Obesity Paradox: Impact of Obesity on the Prevalence and Prognosis of Cardiovascular Diseases

  2008cited by this paper
• Effects of probiotics on atopic dermatitis: a randomised controlled trial

  2005cited by this paper
• Design and validation of a histological scoring system for nonalcoholic fatty liver disease

  2005cited by this paper
• Molecular mediators of hepatic steatosis and liver injury.

  2004influential reference
• Safety of Statins: Focus on Clinical Pharmacokinetics and Drug Interactions

  2004cited by this paper
• Lactobacillus GG bacteria ameliorate arthritis in Lewis rats.

  2004cited by this paper
• Nonalcoholic steatohepatitis: Summary of an AASLD Single Topic Conference

  2003cited by this paper
• Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers.

  2003cited by this paper
• Expanding the Natural History of Nonalcoholic Steatohepatitis: from Cryptogenic Cirrhosis to Hepatocellular Carcinoma

  2003cited by this paper
• Expanding the natural history of nonalcoholic steatohepatitis: from cryptogenic cirrhosis to hepatocellular carcinoma.

  2002cited by this paper
• Introduction to pre- and probiotics

  2002cited by this paper
• Effects of Consumption of Probiotics and Prebiotics on Serum Lipid Levels in Humans

  2002cited by this paper
• Nonalcoholic fatty liver disease: a feature of the metabolic syndrome.

  2001cited by this paper
• Prevalence of and Risk Factors for Hepatic Steatosis in Northern Italy

  2000cited by this paper
• [Estimation of contribution of changes in coronary care to improving survival, event rates, and coronary heart disease mortality across the WHO MONICA Project populations].

  2000cited by this paper
• The role of probiotic bacteria in cancer prevention.

  2000cited by this paper
• Estimation of contribution of changes in coronary care to improving survival, event rates, and coronary heart disease mortality across the WHO MONICA Project populations.

  2000cited by this paper
• Effect of fermented milk (yogurt) containing Lactobacillus acidophilus L1 on serum cholesterol in hypercholesterolemic humans.

  1999influential reference
• Increased Levels of Nuclear SREBP-1c Associated with Fatty Livers in Two Mouse Models of Diabetes Mellitus*

  1999cited by this paper
• Nonalcoholic fatty liver disease: a spectrum of clinical and pathological severity.

  1999influential reference
• Nonalcoholic fatty liver disease

  1999cited by this paper
• Nonalcoholic fatty liver disease: assessment of variability in pathologic interpretations.

  1998cited by this paper
• Statins do more than just lower cholesterol.

  1996cited by this paper
• A single cholesterol measurement underestimates the risk of coronary heart disease. An empirical example from the Lipid Research Clinics Mortality Follow-up Study.

  1990influential reference
• Variation in susceptibility to atherosclerosis among inbred strains of mice.

  1985cited by this paper
• THE WORLD HEALTH ORGANIZATION

  1954cited by this paper

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  2026cites this paper
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  2025cites this paper
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• A novel approach in reducing hypercholesterolemia using modified mango kernel starch and its role as a potential fat replacer.

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  2024cites this paper
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• Harnessing the power of probiotic strains in functional foods: nutritive, therapeutic, and next-generation challenges

  2024cites this paper
• Hypocholesterolemic potential of Bacillus amyloliquefaciens KAVK1 modulates lipid accumulation on 3T3-L1 adipose cells and high fat diet-induced obese rat model

  2024cites this paper
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  2024cites this paper
• Effects of Fermented Goat Milk on Adiposity and Gut Microbiota in a Diet-Induced Obesity Murine Model

  2024cites this paper
• Intestinal Barrier Dysfunction and Gut Microbiota in Non-Alcoholic Fatty Liver Disease: Assessment, Mechanisms, and Therapeutic Considerations

  2024cites this paper
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• Yogurt Supplementation Can Ameliorate Fatty Liver Diseases and Metabolic Syndrome in High Fat‐Induced Conditions in Mice

  2024cites this paper
• An overview of postbiotics: unveiling their distinct role in gut health

  2024cites this paper
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  2024cites this paper
• Lipid-lowering and antioxidant properties of probiotic Bifidobacterium animalis MSMC83 in rats on a high-fat diet.

  2024cites this paper
• Potential benefit of Lactobacillus acidophilus supplementation to rats fed with a high-fat diet on serum lipid profile, kidney amyloid protein and tumor necrosis factor-alpha level.

  2024cites this paper
• Evaluation of Lactobacillus acidophilus and Bacillus coagulans against Simvastatin-induced Rhabdomyolysis in Hypercholesterolemic Rats

  2024cites this paper
• A high-fat diet changes placental morphology but does not change biochemical parameters, placental oxidative stress or cytokine levels.

  2023cites this paper
• Composition and evolutionary characterization of the gut microbiota in pigs

  2023cites this paper
• Effect of hemp seed oil on lipid metabolism in rats fed a high-cholesterol diet

  2023cites this paper
• Weizmannia coagulans JA845 improves atherosclerosis induced by vitamin D3 and high-fat diet in rats through modulating lipid metabolism, oxidative stress, and endothelial vascular injury.

  2023cites this paper
• Beneficial Effects of Indigenous Probiotics in High-Cholesterol Diet-Induced Hypercholesterolemic Rats

  2023cites this paper
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  2023cites this paper
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  2023cites this paper
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  2023cites this paper
• Microbiological quality of probiotic products

  2023cites this paper
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  2023cites this paper
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  2022influential citation
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  2022cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
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  2020cites this paper
• Effects of Lactobacillus on hyperlipidemia in high-fat diet-induced mouse model

  2020cites this paper
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  2019cites this paper
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  2019cites this paper
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  2019cites this paper
• Mining Human Microbiome for Therapeutics

  2019cites this paper
• A Review on Probiotic and Health Benefits of Probiotics

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  2019cites this paper
• Hypocholesterolemic potential of probiotics: Concept and mechanistic insights

  2019cites this paper
• Hepatoprotective effects of synbiotic soy yogurt on mice fed a high-cholesterol diet.

  2019cites this paper
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  2017cites this paper