4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity.

No abstract is available for this paper.

• Publication year

  1996

• Venue

  European Journal of Biochemistry

• Publication date

  1996-07-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1111/J.1432-1033.1996.0469U.XPMID 8706756
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• pH-dependent structural changes in the active site of p-hydroxybenzoate hydroxylase point to the importance of proton and water movements during catalysis.

  1996cited by this paper
• Structure and function of mutant Arg44Lys of 4-hydroxybenzoate hydroxylase implications for NADPH binding.

  1995cited by this paper
• Structure and Function of Mutant Arg44Lys of 4‐Hydroxybenzoate Hydroxylase

  1995cited by this paper
• Protein Modeling by E-mail

  1995cited by this paper
• Structure and mechanism of para‐hydroxybenzoate hydroxylase

  1995cited by this paper
• Dehalogenation of 4-chlorobenzoate

  1995cited by this paper
• Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate,2,4-dihydroxybenzoate, and 2-hydroxy-4-aminobenzoate and of the Tyr222Ala mutant complexed with 2-hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring.

  1994cited by this paper
• The mobile flavin of 4-OH benzoate hydroxylase.

  1994cited by this paper
• Catabolism of 4-hydroxybenzoate in Candida parapsilosis proceeds through initial oxidative decarboxylation by a FAD-dependent 4-hydroxybenzoate 1-hydroxylase.

  1994cited by this paper
• Selective cysteine-->serine replacements in p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens allow the unambiguous assignment of Cys211 as the site of modification by spin-labeled p-chloromercuribenzoate.

  1994cited by this paper
• Cloning and sequencing show that 4-hydroxybenzoate hydroxylase (PobA) is required for uptake of 4-hydroxybenzoate in Rhizobium leguminosarum.

  1994cited by this paper
• Catabolism of 4-hydroxybenzoate in proceeds through initial oxidative decarboxylation by a FAD-dependent 4-hydroxybenzoate 1-hydroxylase

  1994cited by this paper
• Properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Moraxella sp.

  1993cited by this paper
• Role of Tyr201 and Tyr385 in substrate activation by p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.

  1993cited by this paper
• Purification and Properties of 3 Types of Monohydroxybenzoate Oxygenase from Rhodococcus erythropolis S-1

  1993cited by this paper
• Cloning, nucleotide sequence, and expression of a p-hydroxybenzoate hydroxylase isozyme gene from Pseudomonas fluorescens.

  1993cited by this paper
• Evolutionary divergence of pobA, the structural gene encoding p-hydroxybenzoate hydroxylase in an Acinetobacter calcoaceticus strain well-suited for genetic analysis.

  1993cited by this paper
• Substitution of Arg214 at the substrate-binding site of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.

  1992cited by this paper
• Assessment of protein models with three-dimensional profiles

  1992cited by this paper
• Ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl:adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases.

  1992cited by this paper
• Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS-3.

  1992cited by this paper
• Functional and evolutionary relationships among diverse oxygenases.

  1992cited by this paper
• Catalytic function of tyrosine residues in para-hydroxybenzoate hydroxylase as determined by the study of site-directed mutants.

  1991cited by this paper
• Resolution of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 into three components

  1991cited by this paper
• Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region.

  1991cited by this paper
• A program to produce both detailed and schematic plots of protein structures

  1991cited by this paper
• Separate cloning and expression analysis of two protein components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. C8S3.

  1991cited by this paper
• Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases

  1991cited by this paper
• DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergence

  1990cited by this paper
• Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints.

  1990cited by this paper
• The temperature and pH dependence of some properties of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.

  1989cited by this paper
• The coenzyme analogue adenosine 5-diphosphoribose displaces FAD in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation.

  1989cited by this paper
• Crystal structure of the p-hydroxybenzoate hydroxylase-substrate complex refined at 1.9 A resolution. Analysis of the enzyme-substrate and enzyme-product complexes.

  1989cited by this paper
• Sequence and organization of pobA, the gene coding for p-hydroxybenzoate hydroxylase, an inducible enzyme from Pseudomonas aeruginosa.

  1988cited by this paper
• Chemical modification of tyrosine-38 in p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens by 5'-p-fluorosulfonylbenzoyladenosine: a probe for the elucidation of the NADPH binding site? Involvement in catalysis, assignment in sequence and fitting to the tertiary structure.

  1988cited by this paper
• The elucidation of the microheterogeneity of highly purified p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens by various biochemical techniques.

  1987cited by this paper
• Bacterial evolution

  1987cited by this paper
• Prediction of the occurrence of the ADP-binding beta alpha beta-fold in proteins, using an amino acid sequence fingerprint.

  1986cited by this paper
• Prediction of the Occurrence of the ADP-binding βαβ-fold in Proteins, Using an Amino Acid Sequence Fingerprint

  1986cited by this paper
• Cloning of Pseudomonas sp. strain CBS3 genes specifying dehalogenation of 4-chlorobenzoate

  1986cited by this paper
• Absolute stereochemistry of flavins in enzyme-catalyzed reactions.

  1986cited by this paper
• Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

  1985cited by this paper
• Purification and properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Corynebacterium cyclohexanicum.

  1985cited by this paper
• Dehalogenase genes of Pseudomonas putida PP3 on chromosomally located transposable elements.

  1985cited by this paper
• The importance of monopole-monopole and monopole-dipole interactions on the binding of NADPH and NADPH analogues to p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Effects of pH and ionic strength.

  1984cited by this paper
• Incorporation of [18O]water into 4-hydroxybenzoic acid in the reaction of 4-chlorobenzoate dehalogenase from pseudomonas spec. CBS 3.

  1984cited by this paper
• Comparison of the three-dimensional protein and nucleotide structure of the FAD-binding domain of p-hydroxybenzoate hydroxylase with the FAD- as well as NADPH-binding domains of glutathione reductase.

  1983cited by this paper
• Complete nucleotide sequence of the metapyrocatechase gene on the TOI plasmid of Pseudomonas putida mt-2.

  1983cited by this paper
• Primary structure of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.

  1982cited by this paper
• Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants.

  1982cited by this paper
• A RAPID ALKALINE EXTRACTION PROCEDURE FOR SCREENING RECOMBINANT DNA

  1979cited by this paper
• Regulatory sequences involved in the promotion and termination of RNA transcription.

  1979cited by this paper
• A study of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Improved purification, relative molecular mass, and amino acid composition.

  1979cited by this paper
• Crystal structure of p-hydroxybenzoate hydroxylase.

  1979cited by this paper
• A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

  1979cited by this paper
• DNA sequencing with chain-terminating inhibitors.

  1977cited by this paper
• The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

  1974cited by this paper
• On the interaction of para‐hydroxybenzoate hydroxylase from Pseudomonas fluorescens with halogen ions

  1973cited by this paper
• Calcium-dependent bacteriophage DNA infection.

  1970cited by this paper
• Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

  1969cited by this paper
• A procedure for the isolation of deoxyribonucleic acid from micro-organisms

  1961cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper
• COLORIMETRIC DETERMINATION OF THE COMPONENTS OF 3,4-DIHYDROXYPHENYLALANINETYROSINE MIXTURES

  1937cited by this paper

• Microbial synergistic metabolic mechanism of enhanced tetrabromobisphenol A removal by bio-electrochemical system coupled constructed wetland.

  2025cites this paper
• Microbial diversity analysis of municipal solid waste landfills soils of Delhi (NCR) and plastic dump sites of Uttar Pradesh region of India and their function prediction for plastic degrading enzymes

  2025cites this paper
• Class A flavoprotein monooxygenases: Checkpoint and new horizons.

  2025cites this paper
• Transcriptomic profiling of Pseudomonas migulae revealed gene regulatory properties during biodegradation of aromatic hydrocarbons under cold stress

  2025cites this paper
• Structure, dimeric conformation, and coenzyme versatility of p-hydroxybenzoate hydroxylase from Arthrobacter sp. PAMC25564.

  2024cites this paper
• Isolation and Genomic Analysis of 3-Chlorobenzoate-Degrading Bacteria from Soil

  2023cites this paper
• Natural diversity of FAD-dependent 4-hydroxybenzoate hydroxylases.

  2021cites this paper
• Computationally Prospecting Potential Pathways from Lignin Monomers and Dimers toward Aromatic Compounds.

  2021cites this paper
• Identification of Extractives from Various Poplar Species

  2021cites this paper
• Phenolic acid-degrading Paraburkholderia prime decomposition in forest soil

  2020cites this paper
• Metabolic profile analysis and kinetics of p-cresol biodegradation by an indigenous Pseudomonas citronellolis NS1 isolated from coke oven wastewater

  2020cites this paper
• Paraburkholderia madseniana sp. nov., a phenolic acid-degrading bacterium isolated from acidic forest soil.

  2020cites this paper
• Simultaneous 3-/4-Hydroxybenzoates Biodegradation and Arsenite Oxidation by Hydrogenophaga sp. H7

  2019cites this paper
• Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions

  2019cites this paper
• Modular Engineering of Biomass Degradation Pathways

  2019cites this paper
• Characterization of the genome of a Nocardia strain isolated from soils in the Qinghai-Tibetan Plateau that specifically degrades crude oil and of this biodegradation.

  2018cites this paper
• Pyridine Nucleotide Coenzyme Specificity of p-Hydroxybenzoate Hydroxylase and Related Flavoprotein Monooxygenases

  2018cites this paper
• Hot spots for the protein engineering of Baeyer-Villiger monooxygenases.

  2017cites this paper
• Strain HR199 sp. Pseudomonas Vanillin Catabolism in 3,4-Dioxygenase, Which Are Essential for

  2014cites this paper
• Molecular characterization of the enzymes involved in the degradation of a brominated aromatic herbicide

  2013cites this paper
• Structure and mechanism of kynurenine 3-monooxygenase, a candidate huntington’s disease drug target

  2013cites this paper
• Genomic and Functional Analyses of the Gentisate and Protocatechuate Ring-Cleavage Pathways and Related 3-Hydroxybenzoate and 4-Hydroxybenzoate Peripheral Pathways in Burkholderia xenovorans LB400

  2013cites this paper
• OnpA, an Unusual Flavin-Dependent Monooxygenase Containing a Cytochrome b5 Domain

  2012cites this paper
• Metabolic reconstruction of aromatic compounds degradation from the genome of the amazing pollutant-degrading bacterium Cupriavidus necator JMP134.

  2008cites this paper
• Metabolic Reconstruction Ofaromatic Compounds Degradation from the Genome of the Amazing Pollutant-degrading Bacterium Cupriavidus Necator Jmp134

  2007cites this paper
• A Systematic and Comprehensive Combinatorial Approach to Simultaneously Improve the Activity, Reaction Specificity, and Thermal Stability of p-Hydroxybenzoate Hydroxylase*

  2007cites this paper
• Transient Accumulation of Metabolic Intermediates of p-Cresol in the Culture Medium by a Pseudomonas sp. Strain A Isolated from a Sewage Treatment Plant

  2005cites this paper
• Cloning and Sequence Analysis of the 4-Hydroxybenzoate 3-Hydroxylase Gene from a Cyclohexanecarboxylate-degrading Gram-positive Bacterium, "Corynebacterium cyclohexanicum" Strain ATCC 51369

  2005cites this paper
• The purification and characterisation of 4-chlorobenzoate:CoA ligase and 4-chlorobenzoyl CoA dehalogenase from Arthrobacter sp. strain TM-1

  2004cites this paper
• Biodegradation of synthetic surfactants : linear alkylbenzenesulfonates (LAS) and related compounds

  2003cites this paper
• Flavoenzyme-catalyzed oxygenations and oxidations of phenolic compounds

  2002cites this paper
• Aerobic Metabolism of 4-Hydroxybenzoic Acid in Archaea via an Unusual Pathway Involving an Intramolecular Migration (NIH Shift)

  2002cites this paper
• Analysis of the pobA and pobR genes controlling expression of p-hydroxybenzoate hydroxylase in Azotobacter chroococcum.

  2001cites this paper
• Engineering of histidine tail in the N-terminal region of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens

  2001cites this paper
• Gene Cluster on pAO1 of Arthrobacter nicotinovorans Involved in Degradation of the Plant Alkaloid Nicotine: Cloning, Purification, and Characterization of 2,6-Dihydroxypyridine 3-Hydroxylase

  2001cites this paper
• Purification and Properties of p-Hydroxybenzoate Hydroxylases from Rhodococcus Strains

  2001cites this paper
• Switch of Coenzyme Specificity of p-Hydroxybenzoate Hydroxylase

  1999cites this paper
• Molecular Characterization of the GenespcaG and pcaH, Encoding Protocatechuate 3,4-Dioxygenase, Which Are Essential for Vanillin Catabolism inPseudomonas sp. Strain HR199

  1999cites this paper
• Switch of coenzyme specificity of p-hydroxybenzoate hydroxylase.

  1999cites this paper
• Interdomain binding of NADPH in p-Hydroxybenzoate Hydroxylase as Suggested by Kinetic, Crystallographic and Modeling Studies of Histidine 162 and Arginine 269 Variants*

  1998cites this paper
• Lys42 and Ser42 variants of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens reveal that Arg42 is essential for NADPH binding.

  1998cites this paper
• Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase.

  1997cites this paper
• Identification of a novel conserved sequence motif in flavoprotein hydroxylases with a putative dual function in FAD/NAD(P)H binding

  1997cites this paper
• Purification and properties of 4-hydroxybenzoate 1-hydroxylase (decarboxylating), a novel flavin adenine dinucleotide-dependent monooxygenase from Candida parapsilosis CBS604

  1997cites this paper