Genomic typing and phylogenetic analysis of canine parvovirus detected in the state of Odisha, India.

Canine parvovirus type 2 (CPV-2) comprises three major antigenic variants CPV-2a, CPV-2b and CPV-2c. Their mutated variants in geographically distinct locations need to be investigated to understand viral evolution and for development of effective management measures. In the present study, 71 faecal and 12 blood samples from suspected dogs in the state of Odisha, India were analyzed by PCR. Faecal lysate, extracted by the fast boiling method was found to be more sensitive as a template for PCR compared to DNA extracted from faecal samples by the phenol-chloroform method. The results revealed 29 positive cases (583 bp amplicon) out of 71 faecal samples, and 5 positive cases out of 12 blood samples examined, with a few variations in the results from blood and faecal samples in the same cases, thus suggesting the necessity of screening both blood and faecal samples for diagnosis. Restriction digestion of the 583 bp PCR amplicon with MboII (PCR-RFLP) confirmed the strain not to be CPV-2c. Further sequencing of the 583 bp fragments recognized the variant as one of the mutated CPV-2a strain. Interestingly, an additional presence of CPV-2a mutant of 525 bp was observed in eleven *Corresponding author: Dr. Monalisa Behera, PhD Scholar, Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India, Phone: +91 943 820 8810; E-mail: drmonalisabehera@gmail.com

• Publication year

  2016

• Venue

  Veterinarski Arhiv

• Publication date

  2016-09-23

• Fields of study

  Biology, Medicine

• Identifiers

  No identifiers available.

• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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