Identification of the mstE Gene Encoding a Glucose-inducible, Low Affinity Glucose Transporter in Aspergillus nidulans*

The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.

• Publication year

  2006

• Venue

  Journal of Biological Chemistry

• Publication date

  2006-03-31

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.M508198200PMID 16418173
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Ammonium-induced internalisation of UapC, the general purine permease from Aspergillus nidulans.

  2004cited by this paper
• Aspergillus niger mstA encodes a high-affinity sugar/H+ symporter which is regulated in response to extracellular pH.

  2004cited by this paper
• The Onset of Carbon Catabolic Repression and Interplay Between Specific Induction and Carbon Catabolite Repression in Aspergillus nidulans

  2004cited by this paper
• A putative high affinity hexose transporter, hxtA, of Aspergillus nidulans is induced in vegetative hyphae upon starvation and in ascogenous hyphae during cleistothecium formation.

  2004cited by this paper
• MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment

  2004cited by this paper
• Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (GATEWAY)

  2004cited by this paper
• Glucose uptake in germinating Aspergillus nidulans conidia: involvement of the creA and sorA genes.

  2003cited by this paper
• Glucose Uptake in Trichoderma harzianum: Role of gtt1

  2003cited by this paper
• Yeast as a Model System for Studying Glucose Transport

  2003cited by this paper
• Onset of Carbon Catabolite Repression in Aspergillus nidulans

  2003cited by this paper
• PRINTS and its automatic supplement, prePRINTS

  2003cited by this paper
• Kinetic studies on glucose and xylose transport in Saccharomyces cerevisiae

  2002cited by this paper
• Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.

  2001cited by this paper
• The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae

  2001cited by this paper
• Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides

  2001cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• FEBS Lett

  2000cited by this paper
• Multiple Hexose Transporters ofSchizosaccharomyces pombe

  2000cited by this paper
• Confocal microscopy of FM4‐64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae

  2000cited by this paper
• Concurrent knock‐out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae

  1999cited by this paper
• Function and Regulation of Yeast Hexose Transporters

  1999cited by this paper
• The Aspergillus nidulans panB gene encodes ketopantoate hydroxymethyltransferase, required for biosynthesis of pantothenate and Coenzyme A

  1999cited by this paper
• Molecular characterization of HymA, an evolutionarily highly conserved and highly expressed protein of Aspergillus nidulans

  1999cited by this paper
• Carbon allocation in ectomycorrhizas: identification and expression analysis of an Amanita muscaria monosaccharide transporter.

  1998cited by this paper
• Major Facilitator Superfamily

  1998cited by this paper
• The molecular genetics of hexose transport in yeasts.

  1997cited by this paper
• Classification of all putative permeases and other membrane multispanners of the Major Facilitator Superfamily encoded by the complete genome of Saccharomyces cerevisiae

  1997cited by this paper
• Amino Acid Residues Responsible for Galactose Recognition in Yeast Gal2 Transporter*

  1997cited by this paper
• rco-3, a gene involved in glucose transport and conidiation in Neurospora crassa.

  1997cited by this paper
• Fungal Spore Germination: Insights from the Molecular Genetics ofAspergillus nidulansandNeurospora crassa

  1997cited by this paper
• Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

  1997cited by this paper
• The hexose transporter family of Saccharomyces cerevisiae

  1996cited by this paper
• Three different regulatory mechanisms enable yeast hexose transporter (HXT) genes to be induced by different levels of glucose

  1995cited by this paper
• Glutamine 161 of Glut1 glucose transporter is critical for transport activity and exofacial ligand binding.

  1994cited by this paper
• Chemostat cultivation as a tool for studies on sugar transport in yeasts.

  1994cited by this paper
• Sugar transport in Saccharomyces cerevisiae.

  1993cited by this paper
• Roles of multiple glucose transporters in Saccharomyces cerevisiae

  1993cited by this paper
• A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport.

  1993cited by this paper
• Amino acid substitutions at tryptophan 388 and tryptophan 412 of the HepG2 (Glut1) glucose transporter inhibit transport activity and targeting to the plasma membrane in Xenopus oocytes.

  1992cited by this paper
• Site-directed mutagenesis of GLUT1 in helix 7 residue 282 results in perturbation of exofacial ligand binding.

  1992cited by this paper
• An inversion truncating the creA gene of Aspergillus nidulans results in carbon catabolite derepression.

  1990cited by this paper
• Physiology and Biochemistry of Plant Cell Walls

  1990cited by this paper
• RAG1 gene of the yeast Kluyveromyces lactis codes for a sugar transporter.

  1990cited by this paper
• Cell wall structure and the skeletal functions of the wall

  1990cited by this paper
• Notes An inversion truncating the creA gene of Aspergillus niduians results in carbon catabolite derepression

  1990cited by this paper
• The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein.

  1988cited by this paper
• Isolation and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Aspergillus nidulans.

  1988cited by this paper
• Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae.

  1984cited by this paper
• Transformation by integration in Aspergillus nidulans.

  1983cited by this paper
• A method for extracting high-molecular-weight deoxyribonucleic acid from fungi.

  1982cited by this paper
• Genetics

  1981cited by this paper
• Handbook of Genetics

  1976cited by this paper
• Carbon catabolite repression in Aspergillos nidulans.

  1975cited by this paper
• Nitrogen metabolite repression in Aspergillus nidulans.

  1973cited by this paper
• Nitrogen metabolite repression in Aspergillus nidulans

  1973cited by this paper
• Regulation of Sugar Transport in Neurospora crassa

  1971cited by this paper
• Properties of the hexose transport systems of Aspergillus nidulans.

  1971cited by this paper
• Kinetic Characteristics of the Two Glucose Transport Systems in Neurospora crassa

  1971cited by this paper
• Sugar transport in Neurospora crassa. II. A second glucose transport system.

  1970cited by this paper
• Sugar transport in Neurospora crassa.

  1970cited by this paper
• Microbiology

  1950cited by this paper

• Uncovering the transcriptional landscape of Fomes fomentarius during fungal-based material production through gene co-expression network analysis

  2025cites this paper
• Implications of carbon catabolite repression for Aspergillus‐based cell factories: A review

  2024cites this paper
• Primary Metabolism of Human Pathogenic Fungi, Importance for Virulence and Potential for Drug Development

  2021cites this paper
• Profile of Membrane Cargo Trafficking Proteins and Transporters Expressed under N Source Derepressing Conditions in Aspergillus nidulans

  2021cites this paper
• Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking

  2021cites this paper
• RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa

  2020cites this paper
• Metabolic Modeling of Fungi

  2020cites this paper
• The fungus Aspergillus niger consumes sugars in a sequential manner that is not mediated by the carbon catabolite repressor CreA

  2018cites this paper
• In Silico Analysis of Putative Sugar Transporter Genes in Aspergillus niger Using Phylogeny and Comparative Transcriptomics

  2018cites this paper
• Forward genetics screen coupled with whole-genome resequencing identifies novel gene targets for improving heterologous enzyme production in Aspergillus niger

  2018cites this paper
• Cell Biology of Hyphal Growth

  2017cites this paper
• Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species

  2017cites this paper
• Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression

  2017cites this paper
• The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans

  2017cites this paper
• Lawrence Berkeley National Laboratory Recent Work Title Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus Permalink

  2017cites this paper
• Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus

  2017cites this paper
• RNAseq reveals hydrophobins that are involved in the adaptation of Aspergillus nidulans to lignocellulose

  2016cites this paper
• Identification of a Novel L-rhamnose Uptake Transporter in the Filamentous Fungus Aspergillus niger

  2016cites this paper
• Identification and characterization of putative xylose and cellobiose transporters in Aspergillus nidulans

  2016cites this paper
• Selective transport between heterogeneous hyphal compartments via the plasma membrane lining septal walls of Aspergillus niger.

  2015cites this paper
• High-Affinity Glucose Transport in Aspergillus nidulans Is Mediated by the Products of Two Related but Differentially Expressed Genes

  2014influential citation
• Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source

  2014cites this paper
• Germination of Aspergillus niger conidia

  2014cites this paper
• Identification of Glucose Transporters in Aspergillus nidulans

  2013cites this paper
• Transcriptome changes initiated by carbon starvation in Aspergillus nidulans.

  2013cites this paper
• Beginning to understand the role of sugar carriers in Colletotrichum lindemuthianum: the function of the gene mfs1

  2013influential citation
• The uptake of carbon sources by Aspergillus niger

  2012cites this paper
• Organic acid produciton in Aspergillus niger and other filamentous fungi

  2012cites this paper
• Hypertonic conditions trigger transient plasmolysis, growth arrest and blockage of transporter endocytosis in Aspergillus nidulans and Saccharomyces cerevisiae

  2011cites this paper
• Mrt, a gene unique to fungi, encodes an oligosaccharide transporter and facilitates rhizosphere competency in Metarhizium robertsii.

  2011cites this paper
• Identification of the CRE-1 Cellulolytic Regulon in Neurospora crassa

  2011cites this paper
• Effect of nutrient status, pH, temperature and water potential on germination and growth of Rhizopus stolonifer and Gilbertella persicaria.

  2011cites this paper
• Production of microbial secondary metabolites: Regulation by the carbon source

  2010cites this paper
• Carbon source regulation of antibiotic production

  2010cites this paper
• Temporal and Spatial Regulation of Gene Expression During Asexual Development of Neurospora crassa

  2010cites this paper
• Mrt, a Gene Unique to Fungi, Encodes an Oligosaccharide Transporter and Facilitates Rhizosphere Competency in Metarhizium robertsii1[C][W]

  2010cites this paper
• Aspergillus fumigatus Catalytic Glucokinase and Hexokinase: Expression Analysis and Importance for Germination, Growth, and Conidiation

  2010cites this paper
• Eisosome Organization in the Filamentous AscomyceteAspergillus nidulans

  2010cites this paper
• Transcriptional profiling for Aspergillusnidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress.

  2009cites this paper
• AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is essential for acetate permease activity in the hyphal fungus Aspergillus nidulans.

  2008cites this paper
• Changes in primary metabolism leading to citric acid overflow in Aspergillus niger

  2007cites this paper
• Consecutive gene deletions in Aspergillus nidulans: application of the Cre/loxP system

  2006cites this paper