Fabrication and optimisation of a fused filament 3D-printed microfluidic platform

A 3D-printed microfluidic device was designed and manufactured using a low cost ($2000) consumer grade fusion deposition modelling (FDM) 3D printer. FDM printers are not typically used, or are capable, of producing the fine detailed structures required for microfluidic fabrication. However, in this work, the optical transparency of the device was improved through manufacture optimisation to such a point that optical colorimetric assays can be performed in a 50 µl device. A colorimetric enzymatic cascade assay was optimised using glucose oxidase and horseradish peroxidase for the oxidative coupling of aminoantipyrine and chromotropic acid to produce a blue quinoneimine dye with a broad absorbance peaking at 590 nm for the quantification of glucose in solution. For comparison the assay was run in standard 96 well plates with a commercial plate reader. The results show the accurate and reproducible quantification of 0–10 mM glucose solution using a 3D-printed microfluidic optical device with performance comparable to that of a plate reader assay.

• Publication year

  2017

• Venue

  Journal of Micromechanics and Microengineering

• Publication date

  2017-02-15

• Fields of study

  Physics, Materials Science, Chemistry, Engineering

• Identifiers
  DOI 10.1088/1361-6439/aa5ae3
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• 3D printed plastic attenuation with layer height

  2017cited by this paper
• Absorbance spectra of microfluidic cuvette glucose sensor

  2017cited by this paper
• Absorbance of 3D printed microfluidic biosensor at 590nm

  2017cited by this paper
• 3D printed plastic attenuation with print speed

  2017cited by this paper
• Percentage intensity difference of transparent 3D plastics

  2017cited by this paper
• Microfluidic-integrated DNA nanobiosensors.

  2016cited by this paper
• The upcoming 3D-printing revolution in microfluidics.

  2016cited by this paper
• 3D-printed supercapacitor-powered electrochemiluminescent protein immunoarray.

  2016cited by this paper
• High density microarrays on Blu-ray discs for massive screening.

  2014cited by this paper
• Recent advancements in chemical luminescence-based lab-on-chip and microfluidic platforms for bioanalysis.

  2014cited by this paper
• 3D printed microfluidic devices with integrated versatile and reusable electrodes.

  2014cited by this paper
• 3D-printed fluidic devices enable quantitative evaluation of blood components in modified storage solutions for use in transfusion medicine.

  2014cited by this paper
• Open-Source 3D-Printable Optics Equipment

  2013cited by this paper
• Crystallization of Amorphous Poly(Lactic Acid) Induced by Vapor of Acetone to Form High Crystallinity and Transparency Specimen

  2013cited by this paper
• Printed optics: 3D printing of embedded optical elements for interactive devices

  2012cited by this paper
• Microfluidic chemical analysis systems.

  2011cited by this paper
• Validation of a centrifugal microfluidic sample lysis and homogenization platform for nucleic acid extraction with clinical samples.

  2010cited by this paper
• Microfluidics Chamber System for Bone Cell Mechanotransduction Study and Bone Tissue Engineering Application

  2009cited by this paper
• An Optical Method for Evaluation of Geometric Fidelity for Anatomically Shaped Tissue-Engineered Constructs

  2009cited by this paper
• Guidelines: Clinical Utilization of Cardiac Biomarker Testing in Heart Failure National Academy of Clinical Biochemistry Laboratory Medicine Practice

  2008cited by this paper
• National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines: clinical utilization of cardiac biomarker testing in heart failure.

  2008cited by this paper
• National Academy of Clinical Biochemistry Laboratory Medicine practice guidelines: Clinical utilization of cardiac biomarker testing in heart failure.

  2007cited by this paper
• A composite sensor array impedentiometric electronic tongue Part I. Characterization.

  2007cited by this paper
• A composite sensor array impedentiometric electronic tongue Part II. Discrimination of basic tastes.

  2007cited by this paper
• Direct Freeform Fabrication of Seeded Hydrogels in Arbitrary Geometries

  2006cited by this paper
• Disposable microfluidic devices: fabrication, function, and application.

  2005cited by this paper
• Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems.

  2004cited by this paper
• Polymer microfluidic devices.

  2002cited by this paper
• A model of peroxidase activity with inhibition by hydrogen peroxide

  1997cited by this paper
• Glucose oxidase: an ideal enzyme

  1992cited by this paper
• Formation of blue chromophore from oxidative coupling of aminoantipyrine with chromotropic acid in the presence of peroxide and horseradish peroxidase

  1981cited by this paper

• Bio-based materials: an alternative to synthetic polymers for the development of lab-on-a-chip devices

  2026cites this paper
• Lectins - valuable bioligands towards sustainable clinical sensing?

  2025cites this paper
• A low-cost 3D-printing based rapid fabrication procedure for modular microfluidic platform

  2025cites this paper
• From layer to building: multiscale modeling of thermo-optical properties in 3D-printed facades

  2024cites this paper
• Assessing the viability of 3D-printed poly(lactic acid) petri dishes: A sustainable alternative for laboratory use

  2024cites this paper
• Technological Advancement and Trend in Selective Bioanalytical Sample Extraction through State of the Art 3-D Printing Techniques Aiming ‘Sorbent Customization as per need’

  2024cites this paper
• Tuning the Solar Performance of Building Facades through Polymer 3D Printing: Toward Bespoke Thermo‐Optical Properties

  2023cites this paper
• Novel Additive Manufactured Multielectrode Electrochemical Cell with Honeycomb Inspired Design for the Detection of Methyl Parathion in Honey Samples

  2023cites this paper
• 3D Printing Magnetophoretic Displays

  2023cites this paper
• 4D-printed needle panel meters coupled with enzymatic derivatization for reading urea and glucose concentrations in biological samples.

  2023cites this paper
• In-situ measurement of anisotropic Young’s modulus in fused deposition modeling printed cantilevers

  2023cites this paper
• Spectroelectrochemical sensing of reaction intermediates and products in an affordable fully 3D printed device.

  2023cites this paper
• The artistic aesthetic and cultural value orientation of 3D printing

  2023cites this paper
• Compatibility of Popular Three-Dimensional Printed Microfluidics Materials with In Vitro Enzymatic Reactions.

  2022cites this paper
• Direct 3D printed biocompatible microfluidics: assessment of human mesenchymal stem cell differentiation and cytotoxic drug screening in a dynamic culture system

  2022cites this paper
• A Solution to the Clearance Problem of Sacrificial Material in 3D Printing of Microfluidic Devices

  2022cites this paper
• Digital transformation and sustainability in healthcare and clinical laboratories

  2022cites this paper
• 3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

  2022cites this paper
• Current and emerging trends in polymeric 3D printed microfluidic devices

  2022cites this paper
• Facilitating Safe FFF 3D Printing: A Prototype Material Case Study

  2022cites this paper
• Green microfluidics in microchemical engineering for carbon neutrality

  2022cites this paper
• Direct coupling of microextraction with instrumental techniques

  2021cites this paper
• 3D printed microfluidic devices: a review focused on four fundamental manufacturing approaches and implications on the field of healthcare

  2021cites this paper
• Microfluidics for nanomedicines manufacturing: an affordable and low-cost 3D printing approach.

  2021cites this paper
• The emerging role of 3D printing in the fabrication of detection systems

  2021cites this paper
• An Integrative 3D printing method for rapid additive manufacturing of a capacitive force sensor

  2021cites this paper
• Fused filament fabrication 3D printed polylactic acid electroosmotic pumps.

  2021cites this paper
• Understanding and improving FDM 3D printing to fabricate high-resolution and optically transparent microfluidic devices.

  2021cites this paper
• Fully 3D printed fluidic devices with integrated valves and pumps for flow injection analysis.

  2021cites this paper
• Additive Manufacturing of Micro-Electro-Mechanical Systems (MEMS)

  2021cites this paper
• A review on advancements in applications of fused deposition modelling process

  2020cites this paper
• Challenges in Bone Tissue Regeneration: Stem Cell Therapy, Biofunctionality and Antimicrobial Properties of Novel Materials and Its Evolution

  2020cites this paper
• Three-Dimensional Printing of Acrylonitrile Butadiene Styrene Microreactors for Photocatalytic Applications

  2020cites this paper
• In-process monitoring of part geometry in fused filament fabrication using computer vision and digital twins

  2020cites this paper
• Smart diagnostics devices through artificial intelligence and mechanobiological approaches

  2020cites this paper
• Microfluidic paper-based device integrated with smartphone for point-of-use colorimetric monitoring of water quality index

  2020cites this paper
• Innovative catalyst integration on transparent silicone microreactors for photocatalytic applications

  2020cites this paper
• Low-cost and simple FDM-based 3D-printed microfluidic device for the synthesis of metallic core–shell nanoparticles

  2020cites this paper
• Towards a completely 3D printed hot wire anemometer

  2020cites this paper
• 3D printed UV/VIS detection systems constructed from transparent filaments and immobilised enzymes

  2020cites this paper
• Optoelectronic detectors for flow analysis systems manufactured by means of rapid prototyping technology.

  2019cites this paper
• Developing protein-based bio-inks for FDM 3D printed microfluidic and millifluidic biosensor applications

  2019cites this paper
• Experimental investigation using vibration testing method to optimize feed parameters of color mixing nozzle for fused deposition modeling color 3D printer

  2019cites this paper
• 3D-Printed Lab-on-a-Chip Diagnostic Systems-Developing a Safe-by-Design Manufacturing Approach

  2019influential citation
• Developing a Safe-by-Design Manufacturing

  2019influential citation
• Optimization design of color mixing nozzle based on multi physical field coupling

  2019cites this paper
• Enhancement of surface reflectivity of fused deposition modeling parts by post-processing

  2019cites this paper
• A simple procedure to produce FDM-based 3D-printed microfluidic devices with an integrated PMMA optical window

  2019cites this paper
• Opportunities for 3D printed millifluidic platforms incorporating on-line sample handling and separation

  2018cites this paper
• Accessing microfluidics through feature-based design software for 3D printing

  2018cites this paper
• Horseradish peroxidase-catalyzed hydrogelation for biomedical applications.

  2018cites this paper
• Inkjet 3D Printing of Labs-on-a-Chip with Optical Detection

  2018influential citation
• Low-Cost Passive Sampling Device with Integrated Porous Membrane Produced Using Multimaterial 3D Printing.

  2018cites this paper
• Approaches to open source 3-D printable probe positioners and micromanipulators for probe stations

  2018cites this paper
• 3D Printed 3D-Microfluidics: Recent Developments and Design Challenges

  2018cites this paper
• 3D-printed miniaturized fluidic tools in chemistry and biology.

  2018cites this paper
• Temperature-dependent electrical resistance of conductive polylactic acid filament for fused deposition modeling

  2018cites this paper