Generation of High‐Specificity Antibodies against Membrane Proteins Using DNA‐Gold Micronanoplexes for Gene Gun Immunization

No abstract is available for this paper.

• Publication year

  2018

• Venue

  Current Protocols in Protein Science

• Publication date

  2018-01-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/cpps.50PMID 29516482PMCID PMC5846111
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer

  2016cited by this paper
• DNA immunization as a technology platform for monoclonal antibody induction

  2016influential reference
• Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization

  2016cited by this paper
• Induced conformational change on ferrocenyl-terminated alkyls and their application as transducers for label-free immunosensing of Alzheimer's disease biomarker

  2016cited by this paper
• Discovery of functional monoclonal antibodies targeting G-protein-coupled receptors and ion channels.

  2016cited by this paper
• Discovery of Functional Antibodies Targeting Ion Channels

  2015cited by this paper
• Generation of recombinant antibody fragments for membrane protein crystallization.

  2015cited by this paper
• Generation of antibodies against membrane proteins.

  2014cited by this paper
• Mouse Monoclonal Antibodies to Transient Receptor Potential Ankyrin 1 Act as Antagonists of Multiple Modes of Channel Activation

  2014cited by this paper
• An improved and robust DNA immunization method to develop antibodies against extra-cellular loops of multi-transmembrane proteins

  2014cited by this paper
• Lipid modulation of ion channels through specific binding sites.

  2014cited by this paper
• Structure‐Based and Fragment‐Based GPCR Drug Discovery

  2014cited by this paper
• Improving DNA vaccine performance through vector design.

  2014cited by this paper
• Immunization method for multi-pass membrane proteins using highly metastatic cell lines.

  2014cited by this paper
• A Novel Monoclonal Anti-CD81 Antibody Produced by Genetic Immunization Efficiently Inhibits Hepatitis C Virus Cell-Cell Transmission

  2013cited by this paper
• Ion channels as therapeutic targets: a drug discovery perspective.

  2013influential reference
• Influences of membrane mimetic environments on membrane protein structures.

  2013cited by this paper
• New concepts and aids to facilitate crystallization.

  2013influential reference
• DNASU plasmid and PSI:Biology-Materials repositories: resources to accelerate biological research

  2013influential reference
• Vector Design for Improved DNA Vaccine Efficacy, Safety and Production

  2013cited by this paper
• Innate Immune Signaling by, and Genetic Adjuvants for DNA Vaccination

  2013cited by this paper
• Investigation of Tumor Frame Shift Antigens for Prophylactic Cancer Vaccine, Cancer Detection and Tumorigenicity

  2012cited by this paper
• Fabs enable single particle cryoEM studies of small proteins.

  2012influential reference
• The future of human DNA vaccines.

  2012cited by this paper
• Enhancement of antibody responses to native G protein-coupled receptors using E. coli GroEL as a molecular adjuvant in DNA immunization.

  2012cited by this paper
• Antigen-binding abilities of anti-nephrin antibody are prescribed by signal sequence of expression vector in genetic immunization

  2011cited by this paper
• Protective Antigens Against Glanders Identified by Expression Library Immunization

  2011cited by this paper
• High-Efficiency Screening of Monoclonal Antibodies for Membrane Protein Crystallography

  2011cited by this paper
• Crystallization chaperone strategies for membrane proteins.

  2011cited by this paper
• Membrane transporters in drug development

  2010cited by this paper
• Self-assembled micronanoplexes for improved biolistic delivery of nucleic acids.

  2009cited by this paper
• The relative immunogenicity of DNA vaccines delivered by the intramuscular needle injection, electroporation and gene gun methods.

  2008influential reference
• Cationic gold microparticles for biolistic delivery of nucleic acids.

  2008cited by this paper
• Drug—target network

  2007cited by this paper
• Mouse model of respiratory Chlamydia pneumoniae infection for a genomic screen of subunit vaccine candidates.

  2006cited by this paper
• Use of genetic immunization to raise antibodies recognizing toxin-related cell surface ADP-ribosyltransferases in native conformation.

  2005cited by this paper
• Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy

  2004cited by this paper
• A truncated form of CKβ8‐1 is a potent agonist for human formyl peptide‐receptor‐like 1 receptor

  2004cited by this paper
• Preparation of primary amine-modified gold nanoparticles and their transfection ability into cultivated cells.

  2004cited by this paper
• Characterization of three CpG oligodeoxynucleotide classes with distinct immunostimulatory activities

  2004cited by this paper
• How lipids affect the activities of integral membrane proteins.

  2004cited by this paper
• CpG DNA as a vaccine adjuvant

  2003cited by this paper
• High-level generation of polyclonal antibodies by genetic immunization

  2003influential reference
• Adjuvant formulations and delivery systems for DNA vaccines.

  2003cited by this paper
• CpG DNA as a vaccine adjuvant

  2003cited by this paper
• Use of DNA immunization to produce polyclonal antibodies against the native human neurokinin-1 receptor for substance P.

  2002cited by this paper
• Thyroid-Stimulating Monoclonal Antibodies

  2002cited by this paper
• Genetic adjuvants for DNA vaccines.

  2001cited by this paper
• Delineation of a CpG Phosphorothioate Oligodeoxynucleotide for Activating Primate Immune Responses In Vitro and In Vivo1

  2000cited by this paper
• Genetic Live Vaccines Mimic the Antigenicity But Not Pathogenicity of Live Viruses

  1999cited by this paper
• The immunology of cutaneous DNA immunization.

  1999cited by this paper
• Nucleic Acid Immunizations

  1998cited by this paper
• DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma

  1998cited by this paper
• Different T helper cell types and antibody isotypes generated by saline and gene gun DNA immunization.

  1997cited by this paper
• "Peptabody": a new type of high avidity binding protein.

  1997cited by this paper
• Covalent Attachment and Derivatization of Poly(l-lysine) Monolayers on Gold Surfaces As Characterized by Polarization−Modulation FT-IR Spectroscopy

  1996cited by this paper
• DNA–based immunization by in vivo transfection of dendritic cells

  1996cited by this paper
• Production of monoclonal antibodies by genetic immunization.

  1994cited by this paper
• Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA.

  1993cited by this paper
• Genetic immunization is a simple method for eliciting an immune response

  1992cited by this paper
• Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles.

  1991cited by this paper
• Studies on the optimal immunization schedule of experimental animals. IV. The optimal age and sex of mice, and the influence of booster injections.

  1990cited by this paper
• Direct gene transfer into mouse muscle in vivo.

  1990cited by this paper
• Complete sequence of the cDNA for human alpha 1-antitrypsin and the gene for the S variant.

  1984cited by this paper

• Advancements in the conservation of the conformational epitope of membrane protein immunogens

  2025cites this paper
• Review: Membrane protein nanodiscs for antibody discovery.

  2025cites this paper
• Chitosan nanoparticles as next-generation carriers for veterinary DNA vaccines: Mechanisms, immune responses, and translational prospects.

  2025cites this paper
• Therapeutic Monoclonal Antibodies against Cancer: Present and Future

  2023cites this paper
• Antibody Therapies Targeting Complex Membrane Proteins

  2021cites this paper
• Bacteriophages as Potential Tools for Use in Antimicrobial Therapy and Vaccine Development

  2021cites this paper
• Phage-based vaccines.

  2019cites this paper
• In vitro affinity maturation of antibody against membrane-bound GPCR molecules

  2019cites this paper
• Low‐Invasive Cell Injection based on Rotational Microrobot

  2019cites this paper