The Effect of Single Mismatches on Primer Extension.

No abstract is available for this paper.

• Publication year

  2018

• Venue

  Clinical Chemistry

• Publication date

  2018-05-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1373/clinchem.2017.282285PMID 29444902
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mismatch extension of DNA polymerases and high-accuracy single nucleotide polymorphism diagnostics by gold nanoparticle-improved isothermal amplification.

  2015cited by this paper
• Exploiting extension bias in polymerase chain reaction to improve primer specificity in ensembles of nearly identical DNA templates.

  2014cited by this paper
• Characterization of denaturation and renaturation of DNA for DNA hybridization

  2014cited by this paper
• A quantitative fluorescence‐based steady‐state assay of DNA polymerase

  2014cited by this paper
• Single-nucleotide polymorphisms and other mismatches reduce performance of quantitative PCR assays.

  2013cited by this paper
• Stopped-flow DNA polymerase assay by continuous monitoring of dNTP incorporation by fluorescence.

  2013cited by this paper
• The original Michaelis constant: translation of the 1913 Michaelis-Menten paper.

  2011cited by this paper
• The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5' nuclease assay.

  2010cited by this paper
• 3'-Protected 2'-deoxynucleoside 5'-triphosphates as a tool for heat-triggered activation of polymerase chain reaction.

  2009cited by this paper
• Quantitative effects of position and type of single mismatch on single base primer extension.

  2009cited by this paper
• Evaluation of 15 Polymerases and Phosphorothioate Primer Modification for Detection of UV-induced C:G to T:A Mutations by Allele-specific PCR¶

  2004cited by this paper
• Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons.

  2004cited by this paper
• Structures of mismatch replication errors observed in a DNA polymerase.

  2004cited by this paper
• Evaluation of 15 Polymerases and Phosphorothioate Primer Modification for Detection of UV‐induced C:G to T:A Mutations by Allele‐specific PCR ¶

  2004cited by this paper
• Thermodynamics of the binding of Thermus aquaticus DNA polymerase to primed-template DNA.

  2003cited by this paper
• Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations

  2003cited by this paper
• Mfold web server for nucleic acid folding and hybridization prediction

  2003cited by this paper
• Discrimination of primer 3'-nucleotide mismatch by taq DNA polymerase during polymerase chain reaction.

  2000cited by this paper
• Nucleotide analogs facilitate base conversion with 3' mismatch primers.

  1999cited by this paper
• Characterization and application to hot start PCR of neutralizing monoclonal antibodies against KOD DNA polymerase.

  1999cited by this paper
• Single-tube genotyping without oligonucleotide probes.

  1999cited by this paper
• Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR.

  1996cited by this paper
• Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene.

  1992cited by this paper
• Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR.

  1992cited by this paper
• Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.

  1990cited by this paper
• Minimizing the time required for DNA amplification by efficient heat transfer to small samples.

  1990cited by this paper
• Base mispair extension kinetics. Comparison of DNA polymerase alpha and reverse transcriptase.

  1990cited by this paper
• Rapid, nonradioactive detection of mutations in the human genome by allele-specific amplification.

  1989cited by this paper
• Differential extension of 3' mispairs is a major contribution to the high fidelity of calf thymus DNA polymerase-alpha.

  1989cited by this paper
• Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS).

  1989cited by this paper
• Comparison between DNA melting thermodynamics and DNA polymerase fidelity.

  1988cited by this paper
• Robust regression using iteratively reweighted least-squares

  1977cited by this paper
• Rectangular Confidence Regions for the Means of Multivariate Normal Distributions

  1967cited by this paper

• The A, B, C, D’s of replicative DNA polymerase fidelity: utilizing high-throughput single-molecule sequencing to understand the molecular basis for DNA polymerase accuracy

  2025cites this paper
• Investigating the Limits of Detection of Environmental DNA From Loggerhead and Hawksbill Turtles

  2025cites this paper
• Assessing the efficiency of COI primers in eDNA metabarcoding for marine metazoan biodiversity.

  2025cites this paper
• Two-Dimensional Polymerase Chain Reaction for Identifying the HLA Alleles Associated with Adverse Drug Reactions.

  2025cites this paper
• Recombinase Polymerase Amplification for Single Nucleotide Polymorphism-Specific Detection of ßC Variant in Sickle Cell Disease.

  2025cites this paper
• Observation Bias in Metabarcoding

  2025cites this paper
• Artificial base mismatches-mediated PCR (ABM-PCR) for detecting clinically relevant single-base mutations

  2025cites this paper
• CoMIT: a bioinformatic pipeline for risk-based prediction of COVID-19 test inclusivity

  2025cites this paper
• Development of lectin gene-based SNAP and ARMS markers as anticancer biomarkers in mutant rodent tuber (Typhonium flagelliforme) of Pekalongan accession, Indonesia

  2025cites this paper
• Structural basis of the phosphoramidate N-benzimidazole group’s influence on modified primer extension efficiency by Taq DNA polymerase

  2025cites this paper
• Advances and applications in sequencing-based pathogen surveillance

  2025cites this paper
• Structural and Thermodynamic Insights for Enhanced SNP Detection Using N-Benzimidazole Oligonucleotides.

  2025cites this paper
• Thermodynamic Determinants in Antibody-Free Nucleic Acid Lateral Flow Assays (AF-NALFA): Lessons from Molecular Detection of Listeria monocytogenes, Mycobacterium leprae and Leishmania amazonensis

  2025cites this paper
• KRAS mutations detection methodology: from RFLP to CRISPR/Cas based methods

  2024cites this paper
• A multi-AS-PCR-coupled CRISPR/Cas12a assay for the detection of ten single-base mutations.

  2024cites this paper
• Phosphoramidate Azole Oligonucleotides for Single Nucleotide Polymorphism Detection by PCR

  2024influential citation
• Two CRISPR/Cas12a-based methods for fast and accurate detection of single-base mutations.

  2023cites this paper
• Sequence terminus dependent PCR for site-specific mutation and modification detection

  2023cites this paper
• Genotyping SARS-CoV-2 Variants Using Ratiometric Nucleic Acid Barcode Panels.

  2023cites this paper
• A novel metric to improve mismatched primer selection and quantification accuracy in amplifying DNA repeats for quantitative polymerase chain reactions

  2023cites this paper
• Development of a TaqMan real-time PCR assay with an internal amplification control for detecting trace amounts of allergenic mango (Mangifera indica) in commercial food products

  2023cites this paper
• Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers

  2023cites this paper
• Small changes make big progress: A more efficient eDNA monitoring method for freshwater fish

  2023cites this paper
• MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing

  2023cites this paper
• Quantitative analysis of DNA methylation using sequence-specific, real-time loop-mediated isothermal amplification.

  2022cites this paper
• The Impact of Accumulated Mutations in SARS-CoV-2 Variants on the qPCR Detection Efficiency

  2022cites this paper
• Design, optimization, and application of multiplex rRT-PCR in the detection of respiratory viruses

  2022cites this paper
• ccelerated cycling PCR: A novel tool for rapid, sensitive and specific detection of single-nucleotide mutation within 30 min.

  2022cites this paper
• A simple approach for detecting HLA‐A*02 alleles in archival formalin‐fixed paraffin‐embedded tissue samples and an application example for studying cancer immunoediting

  2022cites this paper
• A Review of Mitochondrial SNP Determination using Allele-Specific PCR in Forensic Identification

  2022cites this paper
• PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens

  2021cites this paper
• Simple Nested Allele-Specific approach with penultimate mismatch for precise species and sex identification of tiger and leopard

  2021cites this paper
• Detection of genome edits in plants—from editing to seed

  2021cites this paper
• Principles of Identification of Nucleotide Sequences in ISSR Marker Spectra

  2021cites this paper
• Highly selective Cooperative Primers for multiplex detection of 2 rare mutations 3 4 5

  2021cites this paper
• Internal transcribed spacer primer evaluation for vascular plant metabarcoding

  2021cites this paper
• Inclusion of double helix structural oligonucleotide (STexS) results in an enhance of SNP specificity in PCR

  2021cites this paper
• RT-qPCR Diagnostics: The “Drosten” SARS-CoV-2 Assay Paradigm

  2021cites this paper
• Highly selective Cooperative Primers for multiplex detection of rare mutations

  2021cites this paper
• Endonuclease IV-Regulated DNAzyme Motor for Universal Single-nucleotide Variation Discrimination.

  2021cites this paper
• Selenium atom on phosphate enhances specificity and sensitivity of DNA polymerization and detection.

  2021cites this paper
• In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR

  2021influential citation
• Reverse Transcriptase Kinetics for One-step RT-PCR.

  2020cites this paper
• Allele-Specific PCR for KRAS Mutation Detection Using Phosphoryl Guanidine Modified Primers

  2020influential citation
• Genomic variations in SARS-CoV-2 strains at the target sequences of nucleic acid amplification tests

  2020cites this paper
• Genotyping Technologies in Pharmacogenomics

  2020cites this paper
• A Rapid, SuperSelective Method for Detection of Single Nucleotide Variants in Caenorhabditis elegans

  2020cites this paper
• Pharmacogenomics in Precision Medicine: From a Perspective of Ethnic Differences

  2020cites this paper
• A rapid, super-selective method for detection of single nucleotide variants in C. elegans

  2020influential citation
• PANDAA-monium: Intentional violations of conventional qPCR design enables rapid, HIV-1 subtype-independent drug resistance SNP detection

  2019cites this paper
• Rapid assessment of coral cover from environmental DNA in Hawai'i

  2019cites this paper