Structural bases for a complete myotoxic mechanism: crystal structures of two non-catalytic phospholipases A2-like from Bothrops brazili venom.

No abstract is available for this paper.

• Publication year

  2013

• Venue

  Biochimica et Biophysica Acta

• Publication date

  2013-12-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.bbapap.2013.10.009PMID 24145104
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2000cited by this paper
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  1998cited by this paper
• Crystallography & NMR system: A new software suite for macromolecular structure determination.

  1998cited by this paper
• [20] Processing of X-ray diffraction data collected in oscillation mode.

  1997cited by this paper
• Processing of X-ray diffraction data collected in oscillation mode.

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• Experimentally determined hydrophobicity scale for proteins at membrane interfaces

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• Phospholipase A2--a structural review.

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• Phospholipase A2 myotoxins from Bothrops snake venoms.

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  1991cited by this paper
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  1990cited by this paper
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  1990cited by this paper
• Interfacial catalysis: the mechanism of phospholipase A2

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  1985cited by this paper
• A simple method for displaying the hydropathic character of a protein.

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  1980cited by this paper
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