Reliable quantification of rhinovirus species C using real-time PCR

Highlights • Developed and validated 4 in-house assays to reliably determine viral load of several different RV-C genotypes in nasal samples.• Complete homology between assay and target sequence is the most accurate method to determine viral load.• Three or more mismatches between the probe and target sequence results in substantially inaccurate viral load.• These assays facilitate accurate investigations into the role of RV-C load in disease outcome.

• Publication year

  2016

• Venue

  Journal of Virological Methods

• Publication date

  2016-05-20

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.jviromet.2016.05.014PMID 27216896PMCID 7172306
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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