Respiratory motoneuronal activity is altered by injections of picomoles of glutamate into cat brain stem

The local neural circuitry underlying the control of breathing was studied by injecting nanoliter volumes of excitatory amino acids into discrete regions of cat brain stem. Experiments were performed on chloralose-urethane anesthetized, vagotomized, paralyzed, and artificially ventilated cats. Phrenic, intercostal, and recurrent laryngeal nerve discharges were recorded. Multibarrel pipettes were used for recording and pressure ejection of drugs or a dye for marking recording and ejection sites. Ejected volumes were directly monitored for every injection. Injections, proximal to neurons discharging with a respiratory periodicity, of as little of 200 fmol of L-glutamate in 200 pl of saline elicited marked, site-specific increases or decreases in respiratory motoneuronal discharge. N-Methyl-D-aspartic acid and homocysteic acid elicited similar site-specific alterations in respiratory motor output, although some details of the response could differ qualitatively. Responses to all the excitatory agents used were attenuated by concurrent injection of kynurenic acid, DL-2-amino-4- phosphonobutyric acid, or glutamic acid diethyl ester. There was no change in spontaneous phrenic nerve discharge in response to injections of equivalent or larger volumes of saline or lidocaine. These results indicate a heterogeneity in the spatial organization of the brain-stem neural circuitry underlying respiratory control, which has not been described previously. This injection technique may provide a mechanism for probing the neural circuitry underlying other behaviors.

• Publication year

  1986

• Venue

  Journal of Neuroscience

• Publication date

  1986-08-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1523/JNEUROSCI.06-08-02384.1986PMID 2875138PMCID PMC6568763
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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