Minocycline induces protective autophagy in vascular endothelial cells exposed to an in vitro model of ischemia/reperfusion-induced injury

Minocycline has been reported to exhibit advantageous effects on ischemic stroke; however, the precise mechanism of minocycline remains to be established. In the present study, human umbilical vein endothelial cells (HUVECs) were subjected to in vitro simulated ischemia/reperfusion conditions to determine the potential effect of minocycline-induced autophagy on HUVEC damage under oxygen-glucose deprivation/reperfusion (OGD/R). The study demonstrated that minocycline enhanced autophagy in a dose-dependent manner in HUVECs exposed to OGD/R, and only low-dose minocycline protected HUVECs from OGD/R-induced damage. Subsequently, 3-methyladenine (3-MA) was added into the culture media and the protective effect of minocycline was abolished. At the same time, it has been observed that simultaneous treatment with 3-MA also inhibited the autophagy activity induced by minocycline. This finding could suggest that autophagy induced by minocycline serves as one of the potential protective mechanism underlying the beneficial effects of minocycline on ischemic injury.

• Publication year

  2015

• Venue

  Biomedical Reports

• Publication date

  2015-12-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3892/br.2015.554PMID 26893833PMCID 4734200
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Identification of Eusynstyelamide B as a Potent Cell Cycle Inhibitor Following the Generation and Screening of an Ascidian-Derived Extract Library Using a Real Time Cell Analyzer

  2014influential reference
• Rapamycin induces of protective autophagy in vascular endothelial cells exposed to oxygen-glucose deprivation.

  2014cited by this paper
• Cerebral ischemia-reperfusion-induced autophagy protects against neuronal injury by mitochondrial clearance

  2013cited by this paper
• Hypoxia/Aglycemia-Induced Endothelial Barrier Dysfunction and Tight Junction Protein Downregulation Can Be Ameliorated by Citicoline

  2013cited by this paper
• Alpha-lipoic acid pre- and post-treatments provide protection against in vitro ischemia-reperfusion injury in cerebral endothelial cells via Akt/mTOR signaling.

  2012cited by this paper
• Reactive oxygen species and ischemic cerebrovascular disease.

  2012cited by this paper
• Neuronal autophagy in cerebral ischemia – a potential target for neuroprotective strategies ?

  2012cited by this paper
• A Double‐Edged Sword with Therapeutic Potential: An Updated Role of Autophagy in Ischemic Cerebral Injury

  2012cited by this paper
• Curcumin Induces Autophagy to Protect Vascular Endothelial Cell Survival from Oxidative Stress Damage

  2012cited by this paper
• Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

  2012cited by this paper
• Neuronal autophagy in cerebral ischemia — a potential target for neuroprotective strategies?

  2012cited by this paper
• Guidelines for the use and interpretation of assays for monitoring autophagy

  2012influential reference
• Autophagy, inflammation and neurodegenerative disease

  2011cited by this paper
• Matrix Metalloproteinase-9 in an Exploratory Trial of Intravenous Minocycline for Acute Ischemic Stroke

  2011cited by this paper
• Minocycline inhibits the growth of glioma by inducing autophagy

  2011cited by this paper
• Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2

  2009cited by this paper
• Post‐ischemic brain damage: targeting PARP‐1 within the ischemic neurovascular units as a realistic avenue to stroke treatment

  2009cited by this paper
• Minocycline: A neuroprotective agent for hypoxic‐ischemic brain injury in the neonate?

  2009cited by this paper
• Focal cerebral ischemia induces upregulation of Beclin 1 and autophagy-like cell death.

  2008cited by this paper
• Protective role of autophagy in neonatal hypoxia-ischemia induced brain injury.

  2008cited by this paper
• A new penumbra: transitioning from injury into repair after stroke

  2008cited by this paper
• Blood-brain barrier tight junction permeability and ischemic stroke.

  2008cited by this paper
• Autophagy fights disease through cellular self-digestion

  2008cited by this paper
• The Roles of Autophagy in Cerebral Ischemia

  2007cited by this paper
• Cellular mechanisms of the protective effect of polyphenols on the neurovascular unit in strokes.

  2006cited by this paper
• Minocycline as a Neuroprotective Agent

  2005cited by this paper
• Extracellular proteolytic pathophysiology in the neurovascular unit after stroke

  2004cited by this paper
• [Cerebral ischemia].

  2001cited by this paper

• INPP5D Upregulation by Minocycline Mitigates Sepsis-Associated Neuroinflammation and Neuronal Dysfunction Via Microglial Autophagy and Antioxidant Pathways

  2026cites this paper
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  2023cites this paper
• Isolation and identification of angiogenesis-promoting components in Huanglian Jiedu decoction using live cell bio-specific extraction.

  2022cites this paper
• Mechanistic Insight on Autophagy Modulated Molecular Pathways in Cerebral Ischemic Injury: From Preclinical to Clinical Perspective

  2022cites this paper
• The Anticancer Potential of Doxycycline and Minocycline—A Comparative Study on Amelanotic Melanoma Cell Lines

  2022cites this paper
• Targeting autophagy in ischemic stroke: From molecular mechanisms to clinical therapeutics.

  2021cites this paper
• Cell membrane-coated biomimetic magnetic nanoparticles for the bio-specific extraction of components from Gualou Guizhi decoction exhibiting activities against oxygen-glucose deprivation/reperfusion injury.

  2021cites this paper
• Endothelial protective effect of rapamycin against simulated ischemia injury through up-regulation of autophagy and inhibition of endoplasmic reticulum stress

  2020cites this paper
• Minocycline Induces Autophagy and Inhibits Cell Proliferation in LPS-Stimulated THP-1 Cells

  2020cites this paper
• Silencing matrix metalloproteinase 9 exerts a protective effect on astrocytes after oxygen-glucose deprivation and is correlated with suppression of aquaporin-4.

  2020cites this paper
• Endothelial-Specific Deficiency of ATG5 (Autophagy Protein 5) Attenuates Ischemia-Related Angiogenesis.

  2019cites this paper
• The Post-amyloid Era in Alzheimer's Disease: Trust Your Gut Feeling

  2019cites this paper
• Minocycline promotes cardiomyocyte mitochondrial autophagy and cardiomyocyte autophagy to prevent sepsis-induced cardiac dysfunction by Akt/mTOR signaling

  2019cites this paper
• Prognostic and therapeutic potential of Adenylate kinase 2 in lung adenocarcinoma

  2019cites this paper
• The Role of Autophagy in Anti-Wolbachia Antibiotic Therapy

  2019cites this paper
• Minocycline protects against myocardial ischemia/reperfusion injury in rats by upregulating MCPIP1 to inhibit NF-κB activation

  2019cites this paper
• Silencing LncRNA AK 139328 protects vascular endothelial cells from ischemia-reperfusion injury by increasing PI 3 K / Akt signaling

  2018cites this paper
• Manipulation of autophagy: a novelly potential therapeutic strategy for retinal neovascularization

  2018cites this paper
• Botulinum toxin type A induces protective autophagy in human dermal microvascular endothelial cells exposed to an in vitro model of ischemia/reperfusion injury

  2018influential citation
• PD149163 induces hypothermia to protect against brain injury in acute cerebral ischemic rats.

  2017cites this paper
• Effect of Oral Minocycline on Clinical Recovery Process in Patients with Acute Ischemic Stroke: A Randomized Clinical Trial

  2017cites this paper
• Silencing LncRNA AK139328 protects vascular endothelial cells from ischemia-reperfusion injury by increasing PI3K/Akt signaling

  2017cites this paper
• TSLP/TSLPR promote angiogenesis following ischemic stroke via activation of the PI3K/AKT pathway.

  2017cites this paper
• Ghrelin protects adult rat hippocampal neural stem cells from excessive autophagy during oxygen-glucose deprivation.

  2017cites this paper
• Effects of erythropoietin and methylprednisolone on AQP4 expression in astrocytes

  2017cites this paper