Membrane attach complex of complement (MAC): three-dimensional analysis of MAC-phospholipid vesicle recombinants.

The three-dimensional structure of recombinants of the isolated membrane attack complex (MAC) of complement with single bilayer dioleoyllecithin (DOL) vesicles and with dimyristoyllecithin (DML) vesicles was determined. A total of four MAC-vesicle complexes were analyzed by imaging negatively stained specimens at various defined tilting angles under minimal dose conditions in the electron microscope and by computer-aided three-dimensional reconstruction. The information on electron micrographs obtained at 6 degrees angular increments from +60 degrees to -60 degrees was digitized by densitometric scanning, Fourier-transformed, corrected for imaging errors, cross-correlated, and synthesized to the three-dimensional image. All four MAC-vesicle recombinants showed stain penetration into the interior of the vesicle, indicating increased permeability of the bilayer to negative stain. The MAC appeared as a hollow structure of 16-nm height, 2.0-nm wall thickness, and a 3.0-nm torus at the free end with an outer and inner diameter of 20.0 nm and 10.0 nm. In MAC-DOL vesicles the hollow core of the MAC terminated at the membrane-binding site, and only small pores of up to 2.0-nm in diameter penetrated the bilayer. In one MAC-DML vesicle lipid discontinuities on the outer circumference of the MAC binding site mediated stain penetration. The second MAC-DML vesicle showed a channel of approximately 4.0 nm connecting the hollow core of the MAC across the bilayer with the vesicle interior. The results suggest the MAC may mediate increased membrane permeability by protein channel formation in addition to lipid reorientation.

• Publication year

  1982

• Venue

  Journal of Immunology

• Publication date

  1982-05-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.4049/jimmunol.128.5.2353PMID 6174630
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Polymerization of the ninth component of complement (C9): formation of poly(C9) with a tubular ultrastructure resembling the membrane attack complex of complement.

  1982cited by this paper
• Three-dimensional electron microscopy.

  1981cited by this paper
• Membranolysis by the ninth component of human complement.

  1981cited by this paper
• Membrane attack complex of complement. Evidence for its dimeric structure based on hybrid formation.

  1981cited by this paper
• Analysis of solute diffusion across the C5b-9 membrane lesion of complement: evidence that individual C5b-9 complexes do not function as discrete, uniform pores.

  1980cited by this paper
• Life-span and size of the trans-membrane channel formed by large doses of complement.

  1980cited by this paper
• Permeability changes induced in erthrocyte ghost targets by antibody-dependent cytotoxic effector cells: evidence for membrane pores.

  1980cited by this paper
• Membrane attack complex of complement: a structural analysis of its assembly

  1980cited by this paper
• Membrane attack complex of complement: generation of high-affinity phospholipid binding sites by fusion of five hydrophilic plasma proteins.

  1979cited by this paper
• Molecular reorganization of lipid bilayers by complement: a possible mechanism for membranolysis.

  1979cited by this paper
• Studies on the terminal stages of immune hemolysis. VI. Osmotic blockers of differing Stokes' radii detect complement-induced transmembrane channels of differing size.

  1979cited by this paper
• Studies on the terminal stages of immune hemolysis. V. Evidence that not all complement-produced transmembrane channels are equal.

  1979cited by this paper
• C5b-9 dimer: isolation from complement lysed cells and ultrastructural identification with complement-dependent membrane lesions

  1979cited by this paper
• The structural events associated with the attachment of complement components to cell membranes in reactive lysis.

  1978cited by this paper
• Steady-state analysis of tracer exchange across the C5b-9 complement lesion in a biological membrane.

  1978cited by this paper
• Increased ion permeability of planar lipid bilayer membranes after treatment with the C5b-9 cytolytic attack mechanism of complement.

  1976cited by this paper
• Three-dimensional reconstruction of individual negatively stained yeast fatty-acid synthetase molecules from tilt series in the electron microscope.

  1974cited by this paper
• Mechanism of cytolysis by complement.

  1972cited by this paper
• Lesions due to Complement in Lipid Membranes

  1971cited by this paper
• REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS

  1970cited by this paper
• LYSIS OF ERYTHROCYTES BY COMPLEMENT IN THE ABSENCE OF ANTIBODY

  1970cited by this paper

• Quantitative proteome profiling provides evidence of an activation of the complement cascade in ATTR amyloidosis

  2021influential citation
• Complement 9 in amyloid deposits

  2021cites this paper
• The Roles and Contributions of the Complement System in the Pathophysiology of Autoimmune Diseases

  2020cites this paper
• Interactions between Pathways of the Complement System and Neisseria meningitidis

  2018cites this paper
• iTRAQ-Based Proteomics Analysis of Serum Proteins in Wistar Rats Treated with Sodium Fluoride: Insight into the Potential Mechanism and Candidate Biomarkers of Fluorosis

  2016cites this paper
• The complement system: an evolution in progress

  2016cites this paper
• The Regulatory Activities of recombinant Properdin and TSP1 on Platelet and Complement activation

  2016influential citation
• Complement-Coagulation Cross-Talk: A Potential Mediator of the Physiological Activation of Complement by Low pH

  2015cites this paper
• Complement and Complement Regulatory Proteins in Diabetes

  2015cites this paper
• Role of complement and complement regulatory proteins in the complications of diabetes.

  2015cites this paper
• Structural biology of the membrane attack complex.

  2014cites this paper
• The Lectin Pathway of Complement Activation in Cerebral Ischaemia and Reperfusion Injury

  2013cites this paper
• The Molecular Interactions between Two Activation Pathways of Complement are Essential for a Protective Innate Immune Response to Neisseria Meningitidis Infection

  2012cites this paper
• New treatment options for acute edema attacks caused by hereditary angioedema.

  2011cites this paper
• Complement in Infections

  2011cites this paper
• Anti-GD1a antibodies activate complement and calpain to injure distal motor nodes of Ranvier in mice.

  2010cites this paper
• Elektronenbeugung mit dem Transmissions‐Elektronenmikroskop als phasenbestimmendem Diffraktometer — von der Ortsfrequenzfilterung zur dreidimensionalen Strukturanalyse an Ribosomen

  2006cites this paper
• The membrane attack complex

  2005cites this paper
• Cloning of hamster type XVII collagen cDNA, and pathogenesis of anti-type XVII collagen antibody and complement in hamster bullous pemphigoid.

  2002cites this paper
• CRP, a major culprit in complement-mediated tissue damage in acute myocardial infarction?

  2001cites this paper
• C1-Esterase inhibitor: an anti-inflammatory agent and its potential use in the treatment of diseases other than hereditary angioedema.

  2000cites this paper
• Therapeutic inhibition of the complement system.

  1998cites this paper
• Components and Reactivity

  1998cites this paper
• Complement lysis: a hole is a hole.

  1991cites this paper
• Nonamyloidotic fibrillar glomerulopathy and recurrent gestational anasarca.

  1990cites this paper
• CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells

  1989cites this paper
• Erythrocyte membrane protein deficiencies in paroxysmal nocturnal hemoglobinuria.

  1989cites this paper
• Cytotoxic action and other metabolic consequences of terminal complement proteins.

  1988cites this paper
• Complement and Complement Reactions

  1988cites this paper
• [1] Mediators of inflammation: An overview

  1988cites this paper
• The membrane attack complex of complement.

  1986cites this paper
• Number of Hits Necessary for Complement‐Mediated Hemolysis

  1986cites this paper
• Homologous species restriction in lysis of human erythrocytes: a membrane-derived protein with C8-binding capacity functions as an inhibitor.

  1986cites this paper
• Influence of vesicle size on complement-dependent immune damage to liposomes.

  1986cites this paper
• The relationship between channel size and the number of C9 molecules in the C5b-9 complex.

  1985cites this paper
• Platelet membrane glycoprotein IIb-IIIa complex incorporated into phospholipid vesicles. Preparation and morphology.

  1985cites this paper
• Complement

  1985cites this paper
• Mediators of inflammation.

  1985cites this paper
• Effect of erythrocyte membrane modulation by lysolecithin on complement-mediated lysis.

  1984cites this paper
• Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8.

  1984cites this paper
• Membrane attack by complement.

  1984cites this paper
• [Mechanism and medical significance of cellular lysis induced by complement].

  1984cites this paper
• Cell volume and osmotic properties of erythrocytes after complement lysis measured by flow cytometry.

  1983cites this paper
• The membrane attack complex of complement and its precursor proteins lack phospholipase activity.

  1983cites this paper
• Complement pores in erythrocyte membranes. Analysis of C8/C9 binding required for functional membrane damage.

  1983cites this paper
• Three-dimensional reconstruction and averaging of 30 S ribosomal subunits of Escherichia coli from electron micrographs.

  1983cites this paper
• Freeze-fracture analysis of the membrane lesion of human complement

  1983cites this paper
• Electron Diffraction with the Transmission Electron Microscope as a Phase‐Determining Diffractometer—From Spatial Frequency Filtering to the Three‐Dimensional Structure Analysis of Ribosomes

  1983cites this paper
• ON THE SIZE HETEROGENEITY AND MOLECULAR COMPOSITION OF THE TRANS-MEMBRANE CHANNELS PRODUCED BY COMPLEMENT

  1983cites this paper
• Fibrillary renal deposits and nephritis.

  1983cites this paper
• Ultrastructure of the membrane attack complex of complement: detection of the tetramolecular C9-polymerizing complex C5b-8.

  1982cites this paper
• Circular polymerization of the ninth component of complement. Ring closure of the tubular complex confers resistance to detergent dissociation and to proteolytic degradation.

  1982cites this paper
• Strathprints Institutional Repository Anti-gd1a Antibodies Activate Complement and Calpain to Injure Distal Motor Nodes of Ranvier in Mice

  year unknowncites this paper