Microhomology-Mediated High-Throughput Gene Inactivation Strategy for the Hyperthermophilic Crenarchaeon Sulfolobus islandicus

No abstract is available for this paper.

• Publication year

  2017

• Venue

  Applied and Environmental Microbiology

• Publication date

  2017-10-13

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1128/AEM.02167-17PMID 29030445PMCID PMC5734048
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Genetic technologies for extremely thermophilic microorganisms of Sulfolobus, the only genetically tractable genus of crenarchaea

  2017influential reference
• Differentiation and Structure in Sulfolobus islandicus Rod-Shaped Virus Populations

  2017cited by this paper
• Structured Populations of Sulfolobus acidocaldarius with Susceptibility to Mobile Genetic Elements

  2017cited by this paper
• Transcriptome changes in STSV2‐infected Sulfolobus islandicus REY15A undergoing continuous CRISPR spacer acquisition

  2016cited by this paper
• Life Cycle Characterization of Sulfolobus Monocaudavirus 1, an Extremophilic Spindle-Shaped Virus with Extracellular Tail Development

  2016cited by this paper
• The apt/6-Methylpurine Counterselection System and Its Applications in Genetic Studies of the Hyperthermophilic Archaeon Sulfolobus islandicus

  2016cited by this paper
• Harnessing Type I and Type III CRISPR-Cas systems for genome editing

  2015cited by this paper
• Virus-Induced Dormancy in the Archaeon Sulfolobus islandicus

  2015cited by this paper
• Inter‐viral conflicts that exploit host CRISPR immune systems of Sulfolobus

  2014cited by this paper
• An archaeal CRISPR type III-B system exhibiting distinctive RNA targeting features and mediating dual RNA and DNA interference

  2014cited by this paper
• Molecular analysis of the UV-inducible pili operon from Sulfolobus acidocaldarius

  2013cited by this paper
• Reassortment of CRISPR repeat-spacer loci in Sulfolobus islandicus.

  2013cited by this paper
• Augmenting the Genetic Toolbox for Sulfolobus islandicus with a Stringent Positive Selectable Marker for Agmatine Prototrophy

  2013cited by this paper
• Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon

  2013cited by this paper
• Massive Activation of Archaeal Defense Genes during Viral Infection

  2013cited by this paper
• Metal Resistance and Lithoautotrophy in the Extreme Thermoacidophile Metallosphaera sedula

  2012cited by this paper
• Versatile Genetic Tool Box for the Crenarchaeote Sulfolobus acidocaldarius

  2012cited by this paper
• Patterns of Gene Flow Define Species of Thermophilic Archaea

  2012cited by this paper
• A broadly applicable gene knockout system for the thermoacidophilic archaeon Sulfolobus islandicus based on simvastatin selection.

  2012cited by this paper
• A Synthetic Arabinose-Inducible Promoter Confers High Levels of Recombinant Protein Expression in Hyperthermophilic Archaeon Sulfolobus islandicus

  2012cited by this paper
• Recombinogenic Properties of Pyrococcus furiosus Strain COM1 Enable Rapid Selection of Targeted Mutants

  2012cited by this paper
• Sulfolobus Mutants, Generated via PCR Products, Which Lack Putative Enzymes of UV Photoproduct Repair

  2011cited by this paper
• Genome Analyses of Icelandic Strains of Sulfolobus islandicus, Model Organisms for Genetic and Virus-Host Interaction Studies

  2011influential reference
• Revealing the essentiality of multiple archaeal pcna genes using a mutant propagation assay based on an improved knockout method.

  2010cited by this paper
• Discontinuity and Limited Linkage in the Homologous Recombination System of a Hyperthermophilic Archaeon

  2010influential reference
• Biogeography of the Sulfolobus islandicus pan-genome

  2009cited by this paper
• Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus

  2009cited by this paper
• Response of the Hyperthermophilic Archaeon Sulfolobus solfataricus to UV Damage

  2007cited by this paper
• Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea

  2007cited by this paper
• The Genome of Sulfolobus acidocaldarius, a Model Organism of the Crenarchaeota

  2005cited by this paper
• Homologous recombination of exogenous DNA with the Sulfolobus acidocaldarius genome: properties and uses.

  2005cited by this paper
• Targeted disruption of the alpha-amylase gene in the hyperthermophilic archaeon Sulfolobus solfataricus.

  2003cited by this paper
• Geographic Barriers Isolate Endemic Populations of Hyperthermophilic Archaea

  2003cited by this paper
• A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector

  2003cited by this paper
• Targeted Disruption of the α-Amylase Gene in the Hyperthermophilic Archaeon Sulfolobus solfataricus

  2003cited by this paper
• One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

  2000cited by this paper
• A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.

  1993cited by this paper

• Discovery, characterization, and application of chromosomal integration sites in the hyperthermophilic crenarchaeon Sulfolobus islandicus

  2025cites this paper
• Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus

  2024cites this paper
• Computational analysis of genes with lethal knockout phenotype and prediction of essential genes in archaea

  2024cites this paper
• Integrated conjugative plasmid drives high frequency chromosomal gene transfer in Sulfolobus islandicus

  2023cites this paper
• A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus.

  2022cites this paper
• Transposon Insertion Mutagenesis in Hyperthermophilic Crenarchaeon Sulfolobus islandicus.

  2022cites this paper
• Take a Break to Repair: A Dip in the World of Double-Strand Break Repair Mechanisms Pointing the Gaze on Archaea

  2021cites this paper
• Intraspecific antagonism through viral toxin encoded by chronic Sulfolobus spindle-shaped virus

  2021cites this paper
• Comparative CRISPR type III-based knockdown of essential genes in hyperthermophilic Sulfolobales and the evasion of lethal gene silencing

  2020influential citation
• Duplication of leucyl-tRNA synthetase in an archaeal extremophile may play a role in adaptation to variable environmental conditions

  2020cites this paper
• Archaeal DNA Repair Mechanisms

  2020cites this paper
• Haloferax volcanii—a model archaeon for studying DNA replication and repair

  2020cites this paper
• Multi-scale architecture of archaeal chromosomes.

  2020cites this paper
• Surface resistance to SSVs and SIRVs in pilin deletions of Sulfolobus islandicus

  2019cites this paper
• Cell Structure Changes in the Hyperthermophilic Crenarchaeon Sulfolobus islandicus Lacking the S-Layer

  2019cites this paper
• Thermoacidophilic Sulfolobus species as source for extremozymes and as novel archaeal platform organisms.

  2019cites this paper
• Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering

  2019cites this paper
• A viral ring nuclease anti-CRISPR subverts type III CRISPR immunity

  2019cites this paper
• Participation of UV-regulated Genes in the Response to Helix-distorting DNA Damage in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

  2019cites this paper
• Physical and Functional Compartmentalization of Archaeal Chromosomes.

  2019cites this paper
• Novel Sulfolobus Fuselloviruses with Extensive Genomic Variations

  2019influential citation
• DNA repair in the archaea-an emerging picture.

  2018cites this paper
• The essential genome of the crenarchaeal model Sulfolobus islandicus

  2018cites this paper
• Revealing S-layer Functions in the Hyperthermophilic Crenarchaeon Sulfolobus islandicus

  2018cites this paper