Covalent Protein Labeling by SpyTag–SpyCatcher in Fixed Cells for Super‐Resolution Microscopy

No abstract is available for this paper.

• Publication year

  2017

• Venue

  ChemBioChem

• Publication date

  2017-08-04

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/cbic.201700177PMID 28514494PMCID PMC5599254
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis.

  2017cited by this paper
• Molecule Counts in Localization Microscopy with Organic Fluorophores.

  2017cited by this paper
• Versatile protein tagging in cells with split fluorescent protein

  2016cited by this paper
• A scalable strategy for high-throughput GFP tagging of endogenous human proteins

  2016cited by this paper
• Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher.

  2016cited by this paper
• Programmable polyproteams built using twin peptide superglues

  2016cited by this paper
• Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo.

  2015cited by this paper
• Supplemental Information Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo

  2015cited by this paper
• SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging.

  2015cited by this paper
• Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

  2014cited by this paper
• Site‐Specific Protein Labeling with SNAP‐Tags

  2013cited by this paper
• Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle Averaging

  2013cited by this paper
• Diversity of clathrin function: new tricks for an old protein.

  2012cited by this paper
• Aptamers as potential tools for super-resolution microscopy

  2012cited by this paper
• Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin

  2012cited by this paper
• A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

  2012cited by this paper
• A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

  2012cited by this paper
• Nanoscale architecture of integrin-based cell adhesions

  2010cited by this paper
• Superresolution imaging of chemical synapses in the brain.

  2010cited by this paper
• HaloTag: a novel protein labeling technology for cell imaging and protein analysis.

  2008cited by this paper
• Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes

  2007cited by this paper

• Super-resolution microscopy for structural biology

  2025cites this paper
• Fluorescent labeling of proteins in vitro and in vivo using encoded peptide tags

  2025cites this paper
• Production of trehalose using reusable cellulose microsphere immobilized enzymes via SpyTag and SpyCatcher

  2025cites this paper
• Construction of a versatile fusion protein for targeted therapy and immunotherapy

  2024cites this paper
• Noncanonical Amino Acid Tools and Their Application to Membrane Protein Studies

  2024cites this paper
• A Versatile Magnetic Nanoplatform for Plug-and-Play Functionalization: Genetically Programmable Cargo Loading to Bacterial Magnetosomes by SpyCatcher "Click Biology".

  2024cites this paper
• Sneaking in SpyCatcher using cell penetrating peptides for in vivo imaging

  2023cites this paper
• Catcher/Tag Toolbox: Biomolecular Click‐Reactions For Protein Engineering Beyond Genetics

  2023cites this paper
• Efficient biosynthesis of (R)-1-[3,5-bis(trifluoromethyl)phenyl]ethanamine with self-assembled dual-enzyme clusters

  2023cites this paper
• Compact and modular bioprobe: Integrating SpyCatcher/SpyTag recombinant proteins with zwitterionic polymer-coated quantum dots.

  2023cites this paper
• A Magnetosome-Based Platform for Flow Biocatalysis

  2022cites this paper
• Controlled and Stable Patterning of Diverse Inorganic Nanocrystals on Crystalline Two-Dimensional Protein Arrays.

  2021cites this paper
• The WAVE complex associates with sites of saddle membrane curvature

  2021cites this paper
• Accessibility-dependent topology studies of membrane proteins using a SpyTag/SpyCatcher protein-ligation system.

  2021cites this paper
• HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassays

  2020cites this paper
• Site-Specific Linking of an Oligonucleotide to Mono- and Bivalent Recombinant Antibodies with SpyCatcher-SpyTag System for Immuno-PCR

  2020cites this paper
• Power to the protein: enhancing and combining activities using the Spy toolbox

  2020cites this paper
• SpyCatcher/SpyTag-Mediated Self-Assembly of a Supramolecular Complex for Improved Biocatalytic Production of Trehalose

  2019cites this paper
• Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses

  2019cites this paper
• Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins

  2019cites this paper
• Pick a Tag and Explore the Functions of Your Pet Protein.

  2019cites this paper
• Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

  2019cites this paper
• Insider information on successful covalent protein coupling with help from SpyBank.

  2019cites this paper
• Better together: building protein oligomers naturally and by design

  2019cites this paper
• High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy

  2018cites this paper
• Development of a genetically encoded site-specific fluorescent sensor of human cardiac voltage-gated sodium channel inactivation

  2018cites this paper
• Novel routes to defined post translational modifications using non-canonical amino acids

  2018cites this paper
• Bright split red fluorescent proteins with enhanced complementation efficiency for the tagging of endogenous proteins and visualization of synapses

  2018cites this paper
• Modular assembly of proteins on nanoparticles

  2018cites this paper
• Engineering Tunable Dual Functional Protein Cage Nanoparticles Using Bacterial Superglue.

  2018cites this paper
• Switchable Fluorophores for Single-Molecule Localization Microscopy.

  2018cites this paper
• A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking

  2017cites this paper
• Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics

  2017cites this paper
• Unleashing chemical power from protein sequence space toward genetically encoded “click” chemistry

  2017cites this paper