Characterization of the Nucleoside Triphosphate Phosphohydrolase and Helicase Activities of the Reovirus λ1 Protein*

Previous studies have shown that the reovirus λ1 core protein harbors a putative nucleotide-binding motif and exhibits an affinity for nucleic acids. In addition, a nucleoside triphosphate phosphohydrolase activity present in reovirus cores has been recently assigned to λ1 using gene reassortment analysis. In this study, it was demonstrated that the recombinant λ1 protein, expressed in the yeast Pichia pastoris, is able to hydrolyze nucleoside 5′-triphosphates or deoxynucleoside 5′-triphosphates. This activity was absolutely dependent on the presence of a divalent cation, Mg2+ or Mn2+. The protein can also unwind double-stranded nucleic acid molecules in the presence of a nucleoside 5′-triphosphate or deoxynucleoside 5′-triphosphate. These results provide the first biochemical evidence that the reovirus λ1 protein is a nucleoside triphosphate phosphohydrolase/helicase and strongly support the idea that λ1 participates in transcription of the viral genome.

• Publication year

  1997

• Venue

  Journal of Biological Chemistry

• Publication date

  1997-07-18

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.272.29.18298PMID 9218469
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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