Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested. The method was tested on isolated cardiomyocyte cells (CMs) and rat bone marrow stromal cells (MSCs) labelled with SPIO-rhodamine nanoparticles and stained with standard fluorescent dyes. We proved it is possible to use an exponential decrease of fluorescence in time and lifetime parameters for pseudo-colour 3D image mapping of living cells and their compartments that is not a standard function of confocal microscopes.

• Publication year

  2017

• Venue

  European Signal Processing Conference

• Publication date

  2017-08-01

• Fields of study

  Biology, Materials Science, Computer Science, Engineering, Medicine

• Identifiers
  DOI 10.23919/EUSIPCO.2017.8081245
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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