Paternally inherited transgenes are down‐regulated but retain low activity during early embryogenesis in Arabidopsis

We investigated the timing of transgene activation after fertilisation in Arabidopsis following crosses and using two transgenic promoters (from the AtCYCB1 and AtLTP1 genes). Using both a transactivation system and direct transcriptional fusion to drive β‐glucuronidase reporter expression, reciprocal crosses showed a lack of expression of the paternal components. This is consistent with a lack of paternal genome activity previously reported during early seed development in Arabidopsis [Viella‐Calzada et al. (2000) Nature 404, 91–94]. However, transactivation experiments of the BARNASE gene gave evidence that at least some paternal loci retain transcriptional activity, though at a low level, during early embryogenesis.

• Publication year

  2001

• Venue

  FEBS Letters

• Publication date

  2001-11-30

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0014-5793(01)03097-6PMID 11734197
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Transactivation of BARNASE under the AtLTP1 promoter affects the basal pole of the embryo and shoot development of the adult plant in Arabidopsis.

  2001cited by this paper
• Requirement for 3-ketoacyl-CoA thiolase-2 in peroxisome development, fatty acid beta-oxidation and breakdown of triacylglycerol in lipid bodies of Arabidopsis seedlings.

  2001cited by this paper
• Delayed activation of the paternal genome during seed development

  2000cited by this paper
• Unusual core histones specifically expressed in male gametic cells of Lilium longiflorum

  2000cited by this paper
• Male gametic cell-specific expression of H2A and H3 histone genes

  1999cited by this paper
• The "dark side" of chromatin remodeling: repressive effects on transcription.

  1999cited by this paper
• Lack of Enhancer Function in Mammals Is Unique to Oocytes and Fertilized Eggs*

  1999cited by this paper
• A transcription activation system for regulated gene expression in transgenic plants.

  1998cited by this paper
• Stochastic mechanisms in gene expression.

  1997cited by this paper
• Developmental acquisition of enhancer function requires a unique coactivator activity

  1997cited by this paper
• Cell ablation and the analysis of plant development

  1997cited by this paper
• Developmental changes in DNA methylation of the two tobacco pollen nuclei during maturation.

  1997cited by this paper
• Activation of transcription in Drosophila embryos is a gradual process mediated by the nucleocytoplasmic ratio.

  1996cited by this paper
• A chance-selection model for cell differentiation.

  1996cited by this paper
• Pattern Formation in the Arabidopsis Embryo Revealed by Position-Specific Lipid Transfer Protein Gene Expression.

  1996cited by this paper
• The appearance of male gamete-specific histones gH2B and gH3 during pollen development in Lilium longiflorum.

  1995cited by this paper
• Transcriptional coactivators in yeast and beyond.

  1995cited by this paper
• Tissue-Specific Expression of a Gene Encoding a Cell Wall-Localized Lipid Transfer Protein from Arabidopsis

  1994cited by this paper
• Developmental expression of the arabidopsis cyclin gene cyc1At.

  1994cited by this paper
• Barnase and barstar: two small proteins to fold and fit together.

  1989cited by this paper
• Cloning, sequencing and transcription of an inactivated copy of Bacillus amyloliquefaciens extracellular ribonuclease (barnase).

  1985cited by this paper

• Zygote Activation: The Start of the New Generation in Angiosperms

  2022influential citation
• Plant zygote development: recent insights and applications to clonal seeds.

  2021cites this paper
• Maternal regulation of seed growth and patterning in flowering plants.

  2020cites this paper
• Advances in the study of zygote activation in higher plants

  2020cites this paper
• Optimization of a Modular Steroid-Inducible Gene Expression System for Use in Rice

  2019influential citation
• Genetic, molecular and parent-of-origin regulation of early embryogenesis in flowering plants.

  2019cites this paper
• Transcriptional Activation of Arabidopsis Zygotes Is Required for Their Initial Division

  2019cites this paper
• Transcriptional Activation of Arabidopsis Zygotes Is Required for Initial Cell Divisions

  2019cites this paper
• Key factors involved in stress-induced microspore embryogenesis in barley and rapeseed: DNA methylation, arabinogalactan proteins and auxin.

  2016cites this paper
• Maternal Gametophyte Effects on Seed Development in Maize

  2016cites this paper
• Zygotic genome activation in isogenic and hybrid plant embryos.

  2016cites this paper
• Tissue and Organ Initiation in the Plant Embryo: A First Time for Everything.

  2016cites this paper
• Mitochondrial Genetic Manipulation

  2015cites this paper
• Using large SNP datasets to understand the genetic mechanisms of complex traits in Arabidopsis thaliana

  2015cites this paper
• The Maternal-to-Zygotic Transition in Flowering Plants: Evidence, Mechanisms, and Plasticity.

  2015influential citation
• Non-equivalent contributions of maternal and paternal genomes to early plant embryogenesis

  2014cites this paper
• The expression and roles of parent-of-origin genes in early embryogenesis of angiosperms

  2014cites this paper
• Establishment of Early Verification Method for Introduction of the Binary Trans-activation System in Chinese Cabbage (Brassica rapa L. ssp. Pekinensis)

  2013cites this paper
• Maternal and paternal genomes contribute equally to the transcriptome of early plant embryos

  2012cites this paper
• The maternal-to-zygotic transition in higher plants.

  2012cites this paper
• Dynamic changes of transcript profiles after fertilization are associated with de novo transcription and maternal elimination in tobacco zygote, and mark the onset of the maternal-to-zygotic transition.

  2011cites this paper
• Maternal epigenetic pathways control parental contributions to Arabidopsis early embryogenesis.

  2011cites this paper
• Genes of Both Parental Origins Are Differentially Involved in Early Embryogenesis of a Tobacco Interspecies Hybrid

  2011cites this paper
• The Maternal to Zygotic Transition in Animals and Plants

  2009cites this paper
• The Maize Megagametophyte

  2009cites this paper
• Molecular control of autonomous embryo and endosperm development

  2008influential citation
• Epigenetics and Plant Breeding

  2008cites this paper
• The first zygotic division in Arabidopsis requires de novo transcription of thymidylate kinase.

  2008cites this paper
• The maternal to zygotic transition in animals and plants.

  2008cites this paper
• The alpha-N-acetyl-glucosaminidase gene is transcriptionally activated in male and female gametes prior to fertilization and is essential for seed development in Arabidopsis.

  2008cites this paper
• Natural Variation in the Degree of Autonomous Endosperm Formation Reveals Independence and Constraints of Embryo Growth During Seed Development in Arabidopsis thaliana

  2008cites this paper
• Genomic Imprinting in Plants: A Predominantly Maternal Affair

  2007cites this paper
• Chromatin modification and remodeling during early seed development.

  2007cites this paper
• Arabidopsis GLAUCE promotes fertilization-independent endosperm development and expression of paternally inherited alleles

  2007cites this paper
• Equivalent parental contribution to early plant zygotic development.

  2007cites this paper
• Transactivated and chemically inducible gene expression in plants.

  2006cites this paper
• Differential gene expression in egg cells and zygotes suggests that the transcriptome is restructed before the first zygotic division in tobacco

  2006cites this paper
• Improved transcriptional activators and their use in mis-expression traps in Arabidopsis.

  2005cites this paper
• An Egg Apparatus-Specific Enhancer of Arabidopsis, Identified by Enhancer Detection1

  2005cites this paper
• Timing of the Maternal-to-Zygotic Transition during Early Seed Development in Maizew⃞

  2005cites this paper
• Genomic imprinting in plants: the epigenetic version of an Oedipus complex.

  2005cites this paper
• New pOp/LhG4 vectors for stringent glucocorticoid-dependent transgene expression in Arabidopsis.

  2005cites this paper
• pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco.

  2005cites this paper
• EMBRYONIC FACTOR 1 encodes an AMP deaminase and is essential for the zygote to embryo transition in Arabidopsis.

  2005cites this paper
• Role of non-zygotic parental genes in embryogenesis and endosperm development in flowering plants

  2005cites this paper
• Predictable activation of tissue-specific expression from a single gene locus using the pOp/LhG4 transactivation system in Arabidopsis.

  2004cites this paper
• More than a yolk: the short life and complex times of the plant endosperm.

  2004cites this paper
• A Differential Dosage Hypothesis for Parental Effects in Seed Development

  2004cites this paper
• Genomic Imprinting in Plants

  2004cites this paper
• Control of Male Gametophyte Development

  2004cites this paper
• Genetic Regulation of Embryonic Pattern Formation

  2004cites this paper
• Some reflections on double fertilization, from its discovery to the present.

  2003cites this paper
• ARABIDOPSISgametophyticfactor 1 (gfal) MUTANTS: A GENETIC, DEVELOPMENTAL, AND MOLECULAR ANALYSIS

  2003cites this paper
• Expression of Barstar as a selectable marker in yeast mitochondria

  2003cites this paper
• Heterochronic expression of sexual reproductive programs during apomictic development in Tripsacum.

  2003cites this paper
• Unique features of the plant life cycle and their consequences

  2003cites this paper
• Paternal mRNA and protein synthesis coincides with male chromatin decondensation in maize zygotes.

  2002cites this paper
• Remembrance of things past: chromatin remodeling in plant development.

  2002cites this paper
• Genomic imprinting during seed development.

  2002cites this paper
• Seed development (Communication arising): Early paternal gene activity in Arabidopsis

  2001cites this paper
• Maternal and Paternal Genomes Contribute Equally to the Transcriptome of Early Plant Embryos Accessed Terms of Use Detailed Terms

  year unknowncites this paper