Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging.

Measuring the average refractive index (RI) of spherical objects, such as suspended cells, in quantitative phase imaging (QPI) requires a decoupling of RI and size from the QPI data. This has been commonly achieved by determining the object's radius with geometrical approaches, neglecting light-scattering. Here, we present a novel QPI fitting algorithm that reliably uncouples the RI using Mie theory and a semi-analytical, corrected Rytov approach. We assess the range of validity of this algorithm in silico and experimentally investigate various objects (oil and protein droplets, microgel beads, cells) and noise conditions. In addition, we provide important practical cues for the analysis of spherical objects in QPI.

• Publication year

  2018

• Venue

  Optics Express

• Publication date

  2018-03-16

• Fields of study

  Biology, Materials Science, Physics, Mathematics, Medicine

• Identifiers
  DOI 10.1364/OE.26.010729arXiv 1803.06410PMID 29716005
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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