Site‐directed spin labeling of a bacterial chemoreceptor reveals a dynamic, loosely packed transmembrane domain

No abstract is available for this paper.

• Publication year

  2002

• Venue

  Protein Science

• Publication date

  2002-06-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1110/ps.0202502PMID 12021446PMCID PMC2373632
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Structure in the channel forming domain of colicin E1 bound to membranes: The 402–424 sequence

  2008influential reference
• Modeling the transmembrane domain of bacterial chemoreceptors

  2002cited by this paper
• Infrared Spectroscopic Study of Photoreceptor Membrane and Purple Membrane

  2001influential reference
• Single-molecule spectroscopy of the β2 adrenergic receptor: Observation of conformational substates in a membrane protein

  2001cited by this paper
• Molecular motion of spin labeled side chains in alpha-helices: analysis by variation of side chain structure.

  2001cited by this paper
• Transmembrane signaling in bacterial chemoreceptors.

  2001cited by this paper
• The superfamily of chemotaxis transducers: from physiology to genomics and back.

  2001cited by this paper
• Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations.

  2001cited by this paper
• Site-directed solid-state NMR measurement of a ligand-induced conformational change in the serine bacterial chemoreceptor.

  2001influential reference
• Dynamic activation of protein function: A view emerging from NMR spectroscopy

  2001cited by this paper
• Structure of a conserved receptor domain that regulates kinase activity: the cytoplasmic domain of bacterial taxis receptors.

  2000cited by this paper
• Site-directed spin labeling of proteins. Applications to diphtheria toxin.

  2000cited by this paper
• Internal packing of helical membrane proteins.

  2000cited by this paper
• Site-Directed Spin Labeling of Proteins

  2000cited by this paper
• Identifying conformational changes with site-directed spin labeling

  2000cited by this paper
• Crystal structures of spin labeled T4 lysozyme mutants: implications for the interpretation of EPR spectra in terms of structure.

  2000cited by this paper
• Structure of the KcsA potassium channel from Streptomyces lividans: a site-directed spin labeling study of the second transmembrane segment.

  1999cited by this paper
• Signaling domain of the aspartate receptor is a helical hairpin with a localized kinase docking surface: cysteine and disulfide scanning studies.

  1999influential reference
• A piston model for transmembrane signaling of the aspartate receptor.

  1999cited by this paper
• Motion of spin-labeled side chains in T4 lysozyme: effect of side chain structure.

  1999influential reference
• Four-helical-bundle structure of the cytoplasmic domain of a serine chemotaxis receptor

  1999cited by this paper
• Structural features and light-dependent changes in the sequence 306-322 extending from helix VII to the palmitoylation sites in rhodopsin: a site-directed spin-labeling study.

  1999cited by this paper
• Detection of a Conserved α-Helix in the Kinase-docking Region of the Aspartate Receptor by Cysteine and Disulfide Scanning*

  1998influential reference
• Recent advances in site-directed spin labeling of proteins.

  1998influential reference
• Cysteine and disulfide scanning reveals two amphiphilic helices in the linker region of the aspartate chemoreceptor.

  1998influential reference
• A transmembrane form of annexin XII detected by site-directed spin labeling.

  1998cited by this paper
• Direct measurement of small ligand-induced conformational changes in the aspartate chemoreceptor using EPR.

  1998cited by this paper
• fd coat protein structure in membrane environments: structural dynamics of the loop between the hydrophobic trans-membrane helix and the amphipathic in-plane helix.

  1997cited by this paper
• The lipid bilayer determines helical tilt angle and function in lactose permease of Escherichia coli.

  1997cited by this paper
• Analysis of protein structure in intact cells: Crosslinking in vivo between introduced cysteines in the transmembrane domain of a bacterial chemoreceptor

  1997cited by this paper
• The two-component signaling pathway of bacterial chemotaxis: a molecular view of signal transduction by receptors, kinases, and adaptation enzymes.

  1997cited by this paper
• Cysteine and Disulfide Scanning Reveals a Regulatory α-Helix in the Cytoplasmic Domain of the Aspartate Receptor*

  1997influential reference
• Backbone dynamics of the major coat protein of bacteriophage M13 in detergent micelles by 15N nuclear magnetic resonance relaxation measurements using the model-free approach and reduced spectral density mapping.

  1997cited by this paper
• Converting a transmembrane receptor to a soluble receptor: recognition domain to effector domain signaling after excision of the transmembrane domain.

  1997cited by this paper
• Site-directed spin-labeling of transmembrane domain VII and the 4B1 antibody epitope in the lactose permease of Escherichia coli.

  1997cited by this paper
• Site-directed spin labeling demonstrates that transmembrane domain XII in the lactose permease of Escherichia coli is an alpha-helix.

  1996cited by this paper
• Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: a site-directed spin-labeling study.

  1996cited by this paper
• Molecular mechanism of transmembrane signaling by the aspartate receptor: a model.

  1996cited by this paper
• Molecular evolution of the C-terminal cytoplasmic domain of a superfamily of bacterial receptors involved in taxis.

  1996cited by this paper
• The cytoplasmic fragment of the aspartate receptor displays globally dynamic behavior.

  1996influential reference
• Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics.

  1996cited by this paper
• Organization of Diphtheria Toxin T Domain in Bilayers: A Site-Directed Spin Labeling Study

  1996cited by this paper
• Watching proteins move using site-directed spin labeling.

  1996cited by this paper
• Mutational analysis of a transmembrane segment in a bacterial chemoreceptor

  1996cited by this paper
• Quantitative approaches to utilizing mutational analysis and disulfide crosslinking for modeling a transmembrane domain

  1995cited by this paper
• Transmembrane signaling by the aspartate receptor: engineered disulfides reveal static regions of the subunit interface.

  1995cited by this paper
• The N-terminal Cytoplasmic Tail of the Aspartate Receptor Is Not Essential in Signal Transduction of Bacterial Chemotaxis (*)

  1995cited by this paper
• Transmembrane signaling characterized in bacterial chemoreceptors by using sulfhydryl cross-linking in vivo.

  1995cited by this paper
• Three-dimensional structures of the ligand-binding domain of the bacterial aspartate receptor with and without a ligand.

  1995cited by this paper
• A model for transmembrane signalling by the aspartate receptor based on random-cassette mutagenesis and site-directed disulfide cross-linking.

  1995cited by this paper
• Lock On/Off Disulfides Identify the Transmembrane Signaling Helix of the Aspartate Receptor (*)

  1995influential reference
• Identification of functionally important helical faces in transmembrane segments by scanning mutagenesis.

  1995cited by this paper
• Investigation of structure and dynamics in membrane proteins using site-directed spin labeling

  1994cited by this paper
• The three-dimensional structure of the aspartate receptor from Escherichia coli.

  1994cited by this paper
• A collision gradient method to determine the immersion depth of nitroxides in lipid bilayers: application to spin-labeled mutants of bacteriorhodopsin.

  1994cited by this paper
• Deducing the organization of a transmembrane domain by disulfide cross-linking. The bacterial chemoreceptor Trg.

  1994cited by this paper
• Strategies for differential sensory responses mediated through the same transmembrane receptor.

  1993influential reference
• Determination of transmembrane protein structure by disulfide cross-linking: the Escherichia coli Tar receptor.

  1992influential reference
• Helical structure and orientation of melittin in dispersed phospholipid membranes from amide exchange analysis in situ.

  1992cited by this paper
• SPIN LABELED CYSTEINES AS SENSORS FOR PROTEIN‐LIPID INTERACTION AND CONFORMATION IN RHODOPSIN

  1992cited by this paper
• Polarized Fourier transform infrared spectroscopy of bacteriorhodopsin. Transmembrane alpha helices are resistant to hydrogen/deuterium exchange.

  1990cited by this paper
• Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants.

  1990cited by this paper
• Purification of receptor protein Trg by exploiting a property common to chemotactic transducers of Escherichia coli.

  1989cited by this paper
• Hydrophobic organization of membrane proteins.

  1989cited by this paper
• Site-directed cross-linking. Establishing the dimeric structure of the aspartate receptor of bacterial chemotaxis.

  1988cited by this paper
• Global flexibility in a sensory receptor: a site-directed cross-linking approach.

  1987influential reference
• Fourier transform infrared spectroscopic study of the structure and conformational changes of the human erythrocyte glucose transporter.

  1987cited by this paper
• Continuous and stopped flow EPR spectrometer based on a loop gap resonator

  1987cited by this paper
• Analysis of mutations in the transmembrane region of the aspartate chemoreceptor in Escherichia coli.

  1986cited by this paper
• Infrared spectroscopic study of photoreceptor membrane and purple membrane. Protein secondary structure and hydrogen deuterium exchange.

  1986cited by this paper

• Design of a water-soluble transmembrane receptor kinase with intact molecular function by QTY code

  2024cites this paper
• The distal cytoplasmic tail of the influenza A M2 protein dynamically extends from the membrane.

  2019cites this paper
• Bacterial Chemoreceptor Dynamics: Helical Stability in the Cytoplasmic Domain Varies with Functional Segment and Adaptational Modification.

  2016cites this paper
• Elucidating the energetics of bacterial signal transduction: Insights from PhoQ

  2015cites this paper
• Bacterial chemoreceptor dynamics correlate with activity state and are coupled over long distances

  2015cites this paper
• Differential backbone dynamics of companion helices in the extended helical coiled‐coil domain of a bacterial chemoreceptor

  2015cites this paper
• Cys-scanning disulfide crosslinking and bayesian modeling probe the transmembrane signaling mechanism of the histidine kinase, PhoQ.

  2014cites this paper
• CYSTEINE AND SPIN LABEL MODIFICATION AS A MEANS TO EXPLORE PROTEIN DYNAMICS AND STRUCTURE

  2013cites this paper
• Connectable components for protein design

  2013cites this paper
• Influence of Membrane Lipid Composition on a Transmembrane Bacterial Chemoreceptor*

  2012cites this paper
• PhoQ: Structural and Mechanistic Investigations into an Important Bacterial Sensor Kinase

  2012cites this paper
• Transmembrane polar interactions are required for signaling in the Escherichia coli sensor kinase PhoQ

  2010cites this paper
• Diagnostic cross‐linking of paired cysteine pairs demonstrates homologous structures for two chemoreceptor domains with low sequence identity

  2006cites this paper
• Probing protein-peptide binding surfaces using charged stable free radicals and transverse paramagnetic relaxation enhancement (PRE)

  2005cites this paper
• Phosphorylation-dependent changes in structure and dynamics in ERK2 detected by SDSL and EPR.

  2004cites this paper
• Position and ionization state of Asp in the core of membrane-inserted alpha helices control both the equilibrium between transmembrane and nontransmembrane helix topography and transmembrane helix positioning.

  2004cites this paper
• Accessibility of introduced cysteines in chemoreceptor transmembrane helices reveals boundaries interior to bracketing charged residues

  2004cites this paper
• Dynamic motions of CD39 transmembrane domains regulate and are regulated by the enzymatic active site.

  2004cites this paper