Inhibition of macrophage migration by a skin-reactive polysaccharide from BCG culture filtrates.

A skin-reactive polysaccharide isolated from unheated BCG culture filtrates (GAE) inhibited guinea pig peritoneal exudate cell migration to the same degree as PPD in guinea pigs sensitized 2 to 3 months previously. The hydrolyzed polysaccharide and a second polysaccharide, both skin non-reactive, were inactive in this in vitro model of delayed hypersensitivity. The degree of migration inhibition correlated well with skin test size in recently sensitized (2 to 3 months) animals for both polysaccharide and protein antigens but bore no relationship to serum antibodies. With time, the ability of exudate cells from skin-reactive animals to react in vitro to the polysaccharide antigen disappeared while in vitro reactivity to PPD remained. We conclude that GAE, as well as a number of other polysaccharides, is able to induce and elicit delayed hypersensitivity.

• Publication year

  1969

• Venue

  Journal of Immunology

• Publication date

  1969-06-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.102.6.1466PMID 4891552
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• GAE and other polysaccharides can induce and elicit delayed hypersensitivity.

  Confidence 0.91

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• Migration inhibition correlated with skin test size in recently sensitized animals for both polysaccharide and protein antigens, but showed no relationship to serum antibodies.

  Confidence 0.93

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• Hydrolyzed polysaccharide and the second polysaccharide were inactive in the in vitro delayed hypersensitivity model.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• GAE inhibited guinea pig peritoneal exudate cell migration to the same degree as PPD in animals sensitized 2 to 3 months earlier.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review

• delayed hypersensitivity

  immune response

  A cell-mediated immune response assessed here by skin testing and in vitro migration inhibition.

  Aliases: DTH

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• gae

  polysaccharide antigen

  A skin-reactive polysaccharide isolated from unheated BCG culture filtrates and used as the test antigen in the assays.

  Aliases: skin-reactive polysaccharide from unheated BCG culture filtrates

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• guinea pig peritoneal exudate cell migration

  assay

  The in vitro movement of peritoneal exudate cells from guinea pigs used as the readout in the migration inhibition assay.

  Aliases: migration of guinea pig peritoneal exudate cells

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• hydrolyzed polysaccharide

  polysaccharide antigen

  A polysaccharide derivative produced by hydrolysis and tested as an antigen in the migration assay.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• ppd

  protein antigen

  A protein antigen used as the comparator in migration inhibition and skin test responses.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• second polysaccharide

  polysaccharide antigen

  A second polysaccharide preparation tested alongside GAE in the migration inhibition experiments.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• serum antibodies

  measurement

  Circulating antibody levels measured to compare with the migration inhibition and skin test results.

  Aliases: antibody levels in serum

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review
• skin test size

  outcome

  The magnitude of the skin reaction after antigen challenge in sensitized guinea pigs.

  Aliases: skin reaction size

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) review

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