Identification of proteins from two‐dimensional polyacrylamide gels using a novel acid‐labile surfactant

No abstract is available for this paper.

• Publication year

  2002

• Venue

  Proteomics

• Publication date

  2002-07-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1002/1615-9861(200207)2:7<928::AID-PROT928>3.0.CO;2-PPMID 12124938
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• An optimized procedure for detection of proteins on carrier ampholyte isoelectric focusing and immobilized pH gradient gels with imidazole and zinc salts: Its application to the identification of isoelectric focusing separated isoforms by in‐gel proteolysis and mass spectrometry analysis

  2001cited by this paper
• Directed proteomics identifies a plant-specific protein rapidly phosphorylated in response to bacterial and fungal elicitors.

  2001cited by this paper
• Selection and identification of proteins bound to DNA triple-helical structures by combination of 2D-electrophoresis and MALDI-TOF mass spectrometry.

  2001cited by this paper
• In vivo MHC class II presentation of cytosolic proteins revealed by rapid automated tandem mass spectrometry and functional analyses

  2001cited by this paper
• The use of post-source decay in matrix-assisted laser desorption/ionisation mass spectrometry to delineate T cell determinants.

  2001cited by this paper
• Protein identification based on matrix assisted laser desorption/ionization‐post source decay‐mass spectrometry

  2001cited by this paper
• Mass spectrometry compatibility of two‐dimensional gel protein stains

  2001cited by this paper
• Recent advances in capillary electrophoresis/electrospray‐mass spectrometry

  2001cited by this paper
• Determination of the Complete Amino Acid Sequence for the Coat Protein of Brome Mosaic Virus by Time-of-Flight Mass Spectrometry

  2001cited by this paper
• Quantitative analysis of bacterial and mammalian proteomes using a combination of cysteine affinity tags and 15N-metabolic labeling.

  2001cited by this paper
• Analysis of the plant proteome.

  2001cited by this paper
• A two‐dimensional protein map of Caenorhabditis elegans

  2001cited by this paper
• Detection of biomolecules in electrophoresis gels with salts of imidazole and zinc II: A decade of research

  2001cited by this paper
• Proteomic analysis of arabidopsis seed germination and priming.

  2001cited by this paper
• Identification of a juvenile hormone esterase gene by matching its peptide mass fingerprint with a sequence from the Drosophila genome project.

  2001cited by this paper
• Defining the mycoplasma 'cytoskeleton': the protein composition of the Triton X-100 insoluble fraction of the bacterium Mycoplasma pneumoniae determined by 2-D gel electrophoresis and mass spectrometry.

  2001cited by this paper
• Charting the proteomes of organisms with unsequenced genomes by MALDI-quadrupole time-of-flight mass spectrometry and BLAST homology searching.

  2001cited by this paper
• Matrix-assisted laser desorption/ionization mass spectrometric peptide mapping of high molecular weight glutenin subunits 1Bx7 and 1Dy10 in Cheyenne cultivar.

  2001cited by this paper
• Matrix-assisted laser desorption/ionization coupled with quadrupole/orthogonal acceleration time-of-flight mass spectrometry for protein discovery, identification, and structural analysis.

  2001cited by this paper
• Matrix-assisted laser desorption-ionization mass spectrometry peptide mass fingerprinting for proteome analysis: identification efficiency after on-blot or in-gel digestion with and without desalting procedures.

  2001cited by this paper
• Differential expression of molecular chaperones in brain of patients with Down syndrome

  2001cited by this paper
• Mapping and expression pattern analysis of key components of the major histocompatibility complex class I antigen processing and presentation pathway in a representative human renal cell carcinoma cell line

  2001cited by this paper
• Quantitative proteomic analysis using a MALDI quadrupole time-of-flight mass spectrometer.

  2001cited by this paper
• Identification of the apolipoprotein E4 isoform in cerebrospinal fluid with preparative two‐dimensional electrophoresis and matrix assisted laser desorption/ionization‐time of flight‐mass spectrometry

  2001cited by this paper
• Identification of a Phosphorylation Site in the Hinge Region of the Human Progesterone Receptor and Additional Amino-terminal Phosphorylation Sites*

  2001cited by this paper
• Characterization of differently processed forms of enolase 2 from Saccharomyces cerevisiae by two‐dimensional gel electrophoresis and mass spectrometry

  2001cited by this paper
• The proteome of maize leaves: Use of gene sequences and expressed sequence tag data for identification of proteins with peptide mass fingerprints

  2001cited by this paper
• Practical aspects of fluorescent staining for proteomic applications

  2001cited by this paper
• Archived polyacrylamide gels as a resource for proteome characterization by mass spectrometry

  2001cited by this paper
• Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry.

  2000cited by this paper
• Simultaneous analysis of endogenous neurotransmitters and neuropeptides in brain tissue using capillary electrophoresis — microelectrospray‐tandem mass spectrometry

  1999cited by this paper
• Direct analysis of the products of sequential cleavages of peptides and proteins affinity-bound to immobilized metal ion beads by matrix-assisted laser desorption/ionization mass spectrometry.

  1999cited by this paper
• Biochemistry, 2nd ed.

  1999cited by this paper
• An integrated approach to proteome analysis: identification of proteins associated with cardiac hypertrophy.

  1998cited by this paper
• Mass spectrometry and the age of the proteome.

  1998cited by this paper
• Identification of the components of simple protein mixtures by high-accuracy peptide mass mapping and database searching.

  1997cited by this paper
• Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.

  1996cited by this paper
• Two‐dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genome

  1995cited by this paper
• High resolution two-dimensional electrophoresis of proteins.

  1975cited by this paper

• Proteomic Portrait of Degranulation Program in Human Circulating Neutrophils Upon Multi-Inflammatory and Infectious Activation

  2025cites this paper
• Stress-induced premature senescence in high five cell cultures: a principal factor in cell-density effects

  2024cites this paper
• HNRNPA2B1 as a potential therapeutic target for thymic epithelial tumor recurrence: An integrative network analysis

  2023cites this paper
• Proteomic Portrait of Human Lymphoma Reveals Protein Molecular Fingerprint of Disease Specific Subtypes and Progression

  2022cites this paper
• Deciphering tissue‐based proteome signatures revealed novel subtyping and prognostic markers for thymic epithelial tumors

  2020influential citation
• Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives

  2020cites this paper
• Online mass spectrometry of CE (SDS)-separated proteins by two-dimensional capillary electrophoresis

  2019cites this paper
• 1 Discovering seed proteome : challenges and 2 perspectives 3

  2018influential citation
• A Comparison of Methods To Enhance Protein Detection of Lipoproteins by Mass Spectrometry.

  2015cites this paper
• Automated, Online Sample Preparation for LC-MS Analyses: Affinity Capture, Digestion, and Clean-Up

  2015cites this paper
• Ionic liquids as buffer additives in ionic liquid-polyacrylamide gel electrophoresis separation of mixtures of low and high molecular weight proteins

  2015cites this paper
• A New Enhanced, Rapid and Precise Sample Preparation Protocol for Label-free Protein Quantification

  2014cites this paper
• Integrated SDS removal and peptide separation by strong-cation exchange liquid chromatography for SDS-assisted shotgun proteome analysis.

  2012cites this paper
• Mass spectrometry of peptides and proteins from human blood.

  2011cites this paper
• A Straightforward and Robust Method for Introducing Human Hair as a Nucleant into High Throughput Crystallization Trials

  2011cites this paper
• Investigation of solubilization and digestion methods for microsomal membrane proteome analysis using data‐independent LC‐MSE

  2011cites this paper
• Quantitative Mass Spectrometric Approaches

  2011cites this paper
• Comparison of surfactant-assisted shotgun methods using acid-labile surfactants and sodium dodecyl sulfate for membrane proteome analysis.

  2011cites this paper
• Variations on a theme: changes to electrophoretic separations that can make a difference.

  2010cites this paper
• Troglitazone inhibits histone deacetylase activity in breast cancer cells.

  2010cites this paper
• Size‐based protein separations by microchip electrophoresis using an acid‐labile surfactant as a replacement for SDS

  2009cites this paper
• Phase transfer surfactant-aided trypsin digestion for membrane proteome analysis.

  2008cites this paper
• Phase 2 protein inducers in the diet promote healthier aging.

  2008cites this paper
• Capture of an activated receptor complex from the surface of live cells by affinity receptor chromatography.

  2008cites this paper
• Proteins at membrane surfaces-a review of approaches.

  2007cites this paper
• Trends in sample preparation for classical and second generation proteomics.

  2007cites this paper
• Identification of histidine phosphorylations in proteins using mass spectrometry and affinity-based techniques.

  2007cites this paper
• Proteomic analysis of day–night variations in protein levels in the rat pineal gland

  2007cites this paper
• Sample treatment for protein identification by mass spectrometry-based techniques

  2006cites this paper
• Surfactant-enhanced desorption/ionization on silicon mass spectrometry.

  2006cites this paper
• Evaluation of the application of sodium deoxycholate to proteomic analysis of rat hippocampal plasma membrane.

  2006cites this paper
• Analysis of membrane proteins from human chronic myelogenous leukemia cells: comparison of extraction methods for multidimensional LC-MS/MS.

  2006cites this paper
• Proteome analysis of embryo and endosperm from germinating tomato seeds

  2005cites this paper
• Combination detergent/MALDI matrix: functional cleavable detergents for mass spectrometry.

  2005cites this paper
• Proteome analysis of early somatic embryogenesis in Picea glauca

  2005cites this paper
• Fast proteolytic digestion coupled with organelle enrichment for proteomic analysis of rat liver.

  2004cites this paper
• Inaktivierung und Entfernung von Prionen bei der Aufbereitung von Medizinprodukten

  2004cites this paper
• Ion-pair assisted recovery of matrix-assisted laser desorption/ionization mass spectral signals from SDS-containing peptide-protein mixtures.

  2004cites this paper
• Protein Identification by In-Gel Digestion and Mass Spectrometry

  2004cites this paper
• Acid-labile surfactant improves in-sodium dodecyl sulfate polyacrylamide gel protein digestion for matrix-assisted laser desorption/ionization mass spectrometric peptide mapping.

  2004cites this paper
• Acid-labile surfactant assists in-solution digestion of proteins resistant to enzymatic attack.

  2004cites this paper
• Mass spectrometry of intracellular and membrane proteins using cleavable detergents.

  2003cites this paper
• Sodium dodecyl sulfate versus acid‐labile surfactant gel electrophoresis: Comparative proteomic studies on rat retina and mouse brain

  2003cites this paper
• Proteomics in 2002: a year of technical development and wide-ranging applications.

  2003cites this paper
• Current Awareness on Comparative and Functional Genomics

  2003cites this paper