Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing

We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels and to accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq for studying allelic variations in the transcriptome.

• Publication year

  2013

• Venue

  Nature Methods

• Publication date

  2013-11-24

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1038/nmeth.2736PMID 24270603PMCID 3877737
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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