A thermodynamic study of the binding of linear and cyclic oligosaccharides to the maltodextrin-binding protein of Escherichia coli.

Isothermal titration calorimetric (ITC) studies over a range of temperatures of the binding of maltose, maltotriose, maltotetraose and beta-cyclodextrin to the maltodextrin-binding protein (MBP) of Escherichia coli are reported. The binding constants of maltose, maltotriose and beta-cyclodextrin are not very different, namely 8.7 x 10(5), 13.0 x 10(5) and 2.55 x 10(5) M-1, respectively at 25 degrees C. The calorimetric data obtained with maltotetraose cannot be interpreted in terms of a definite binding constant. The binding of maltose and maltotriose is endothermic with a large entropy increase while that of beta-cyclodextrin is exothermic, with a smaller entropy increase. The binding of maltotetraose was endothermic or exothermic depending on the temperature.

• Publication year

  1998

• Venue

  Biophysical Chemistry

• Publication date

  1998-02-16

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0301-4622(97)00113-0PMID 9540203
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Significant discrepancies between van't Hoff and calorimetric enthalpies. III.

  1997cited by this paper
• Thermodynamics of maltose binding protein unfolding

  1997cited by this paper
• Significant discrepancies between van't Hoff and calorimetric enthalpies.

  1995cited by this paper
• Significant discrepancies between van't hoff and calorimetric enthalpies. II

  1995cited by this paper
• Refined 1.8-A structure reveals the mode of binding of beta-cyclodextrin to the maltodextrin binding protein.

  1993cited by this paper
• Crystallographic evidence of a large ligand-induced hinge-twist motion between the two domains of the maltodextrin binding protein involved in active transport and chemotaxis.

  1992cited by this paper
• The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis.

  1992cited by this paper
• Tritium NMR spectroscopy of ligand binding to maltose-binding protein.

  1991cited by this paper
• Thermodynamics of protein-peptide interactions in the ribonuclease S system studied by titration calorimetry.

  1990cited by this paper
• Rapid measurement of binding constants and heats of binding using a new titration calorimeter.

  1989cited by this paper
• Rates of ligand binding to periplasmic proteins involved in bacterial transport and chemotaxis.

  1983cited by this paper
• Thermodynamics of the binding of L-arabinose and of D-galactose to the L-arabinose-binding protein of Escherichia coli.

  1983cited by this paper
• Thermodynamics of the binding of D-glucose to yeast hexokinase.

  1981cited by this paper
• L-Arabinose-binding protein-sugar complex at 2.4 A resolution. Stereochemistry and evidence for a structural change.

  1981cited by this paper
• Glucose-induced conformational change in yeast hexokinase.

  1978cited by this paper

• Synthesis, radiolabeling, and biological evaluation of methyl 6-deoxy-6-[18F]fluoro-4-thio-α-d-maltotrioside as a positron emission tomography bacterial imaging agent

  2025cites this paper
• A Highly Conserved ABC Transporter Mediates Cello-Oligosaccharide Uptake in the Extremely Thermophilic, Lignocellulolytic Bacterium Anaerocellum (f. Caldicellulosiruptor) bescii

  2025cites this paper
• Isothermal titration calorimetry analysis of the binding between the maltodextrin binding protein malE of Staphylococcus aureus with maltodextrins of various lengths.

  2024cites this paper
• Maltodextrin transport in the extremely thermophilic, lignocellulose degrading bacterium Anaerocellum bescii (f. Caldicellulosiruptor bescii)

  2024cites this paper
• Unraveling the molecular mechanism of the interaction between cyclodextrin-based nanoparticles (CDNPs) and paroxetine hydrochloride

  2024cites this paper
• Stability of ligand-induced protein conformation influences affinity in maltose-binding protein

  2021cites this paper
• Targeting galectin-3 by natural glycosides: a computational approach

  2020cites this paper
• Molecular analysis of cyclic α-maltosyl-(1→6)-maltose binding protein in the bacterial metabolic pathway

  2020cites this paper
• Targeting galectin-3 by natural glycosides: a computational approach

  2020cites this paper
• Structural analysis of β‐L‐arabinobiose‐binding protein in the metabolic pathway of hydroxyproline‐rich glycoproteins in Bifidobacterium longum

  2020cites this paper
• Isothermal Analysis of ThermoFluor Data can readily provide Quantitative Binding Affinities

  2019cites this paper
• Molecular characterization of the interaction of sialic acid with the periplasmic binding protein from Haemophilus ducreyi

  2018cites this paper
• Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins

  2018cites this paper
• Inducer exclusion in Firmicutes: insights into the regulation of a carbohydrate ATP binding cassette transporter from Lactobacillus casei BL23 by the signal transducing protein P‐Ser46‐HPr

  2017cites this paper
• Role of Water in Ligand Binding to Maltose-Binding Protein: Insight from a New Docking Protocol Based on the 3D-RISM-KH Molecular Theory of Solvation

  2015cites this paper
• On the molecular basis of the high affinity binding of basic amino acids to LAOBP, a periplasmic binding protein from Salmonella typhimurium

  2015cites this paper
• Large Protein Folding and Dynamics Studied by Advanced Hydrogen Exchange Methods

  2013cites this paper
• Accurate high-throughput structure mapping and prediction with transition metal ion FRET.

  2013influential citation
• Site‐Selective Affinity Labelling of Maltose Binding Protein in Bacterial Cells

  2012cites this paper
• Substrate Transport Activation Is Mediated through Second Periplasmic Loop of Transmembrane Protein MalF in Maltose Transport Complex of Escherichia coli*

  2012influential citation
• Ligand-modulated Parallel Mechanical Unfolding Pathways of Maltose-binding Proteins*

  2011cites this paper
• The molecular basis of glycogen breakdown and transport in Streptococcus pneumoniae

  2010cites this paper
• Unique Sugar Metabolic Pathways of Bifidobacteria

  2010cites this paper
• Immobilization and molecular interactions between bacteriophage and lipopolysaccharide bilayers.

  2010cites this paper
• Structural and Thermodynamic Analyses of Solute-binding Protein from Bifidobacterium longum Specific for Core 1 Disaccharide and Lacto-N-biose I*

  2008cites this paper
• Ligand-induced structural changes in maltose binding proteins measured by atomic force microscopy.

  2008cites this paper
• Making Cool Drugs Hot : The Use of Isothermal Titration Calorimetry as a Tool to Study Binding Energetics application note Ultrasensitive Calorimetry for the Life Sciences TM

  2008cites this paper
• Protein engineering for biosensor development

  2008cites this paper
• MalE of Group A Streptococcus Participates in the Rapid Transport of Maltotriose and Longer Maltodextrins

  2007cites this paper
• Measurements of binding thermodynamics in drug discovery.

  2005cites this paper
• Untersuchungen zum Acarbose-Metabolismus von Actinoplanes sp.: Charakterisierung der Maltose/Maltotriose-Transportaktivitäten sowie eines potentiellen ABC-Transporters für Acarbose

  2004cites this paper
• Detection of low-molecular-weight analytes by surface plasmon resonance and receptor conformational changes

  2002cites this paper
• The crystal structure of a liganded trehalose/maltose-binding protein from the hyperthermophilic Archaeon Thermococcus litoralis at 1.85 A.

  2001cites this paper
• Isothermal titration calorimetry in drug discovery.

  2001cites this paper
• Making cool drugs hot: isothermal titration calorimetry as a tool to study binding energetics.

  2001cites this paper
• Enthalpic barriers to the hydrophobic binding of oligosaccharides to phage P22 tailspike protein.

  2001cites this paper
• Conversion of a maltose receptor into a zinc biosensor by computational design

  2001cites this paper
• Using receptor conformational change to detect low molecular weight analytes by surface plasmon resonance.

  2001cites this paper
• Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein.

  2001cites this paper
• Manipulation of ligand binding affinity by exploitation of conformational coupling

  2001cites this paper
• Molekulare und biochemische Charakterisierung des Trehalose/Maltose-Transportsystems des hyperthermophilen Archaeons Thermococcus litoralis

  2001cites this paper
• Maltose-binding protein from the hyperthermophilic bacterium Thermotoga maritima: stability and binding properties.

  2000cites this paper
• Towards Defining the Role of Glycans as Hardware in Information Storage and Transfer: Basic Principles, Experimental Approaches and Recent Progress

  2000cites this paper
• Large-scale expression and thermodynamic characterization of a glutamate receptor agonist-binding domain.

  2000cites this paper
• A thermodynamic study on the formation and stability of DNA duplex at transcription site for DNA binding proteins GCN4.

  1999cites this paper
• Separation and analysis of peptides and proteins.

  1999cites this paper
• Thermodynamic analysis of biomolecular interactions.

  1999cites this paper
• Structural Basis for Oligosaccharide Recognition by Pyrococcus Furiosus Maltodextrin-binding Protein Protein Engineering Section Macromolecular Crystallography Laboratory

  year unknowncites this paper
• Protein Engineering for Biosensor Development Abstract Protein Engineering for Biosensor Development

  year unknowncites this paper