Automated assay of activities of enzymes involving the diphosphopyridine nucleotide-reduced diphosphopyridine nucleotide reaction.

A continuous flow instrumental system of analysis, first described by Skeggs (l), was previously applied to the determination of phosphohexose isomerase activity and resulted in a method that was approximately eightfold as rapid as the manual procedure (2). The present paper is concerned with the application of this system of analysis to the determination of enzyme activities that utilize the diphosphopyridine nucleotide ti reduced diphosphopyridine nucleotide reaction; assays of lactic acid dehydrogenase, alcohol dehydrogenase, and glutamic oxaloacetic transaminase have been used to illustrate the method of application.

• Publication year

  1961

• Venue

  Journal of Biological Chemistry

• Publication date

  1961-04-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)64269-3PMID 13749113
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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